CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects

Mir Muhammad Adil

CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects

Abstract

Carbapenemase-producing Gram-negative bacteria require rapid detection of resistance determinants to support clinical microbiology and infection-control decisions. CRISPR/Cas12a biosensors offer sequence-specific recognition coupled with collateral cleavage of single-stranded DNA reporters, enabling fluorescence and lateral-flow detection. This focused narrative review examines 11 representative primary studies published between 2020 and 2026, comparing amplification strategies, analytical sensitivity, validation materials, assay configurations, and point-of-care potential. Most platforms target blaKPC and blaNDM, with more limited coverage of blaOXA-48-like, blaVIM, and blaIMP. Reported analytical detection limits vary substantially across purified DNA, cultured isolates, and spiked matrices, limiting direct comparison. Although selected studies report high agreement with molecular reference methods, clinical validation frequently involves small, predefined populations. Recent closed-tube and one-pot configurations reduce handling requirements, but complete sample-to-answer performance remains inconsistently evaluated. Further prospective validation using representative patient specimens, broader gene coverage, internal controls, and standardized reporting is required before routine clinical implementation.

Keywords

CRISPR/Cas12a, Biosensor, Antimicrobial resistance, Carbapenem resistance, Carbapenemase genes, - Point-of-care diagnostics

Supporting Institution

No external funding or institutional financial support was received for this study.

Ethical Statement

Ethical approval was not required because this article is a review of previously published literature and did not involve human participants, animals, or the collection of new data.

Thanks

The author has no acknowledgments to declare.

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Details

Primary Language

English

Subjects

Sensor Technology

Journal Section

Review

Authors

Mir Muhammad Adil ^*
0009-0000-0830-5309
Pakistan

Publication Date

November 15, 2026

Submission Date

September 30, 2026

Acceptance Date

November 10, 2026

Published in Issue

Year 2026 Number: 2026-1

IZ

https://izlik.org/JA66CZ85NZ

APA

Adil, M. M. (2026). CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects. Turkish Journal of Sensors and Biosensors, 2026-1, 1-12. https://izlik.org/JA66CZ85NZ

AMA

1.Adil MM. CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects. Turkish Journal of Sensors and Biosensors. 2026;(2026-1):1-12. https://izlik.org/JA66CZ85NZ

Chicago

Adil, Mir Muhammad. 2026. “CRISPR/Cas12a-Based/Biosensors/for/Rapid/Detection/of/Carbapenem/Resistance/Genes:/Recent/Advances/and/Point-of-Care/Prospects”. Turkish Journal of Sensors and Biosensors, no. 2026-1: 1-12. https://izlik.org/JA66CZ85NZ.

EndNote

Adil MM (November 15, 2026) CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects. Turkish Journal of Sensors and Biosensors 2026-1 1–12.

IEEE

[1]M. M. Adil, “CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects”, Turkish Journal of Sensors and Biosensors, no. 2026-1, pp. 1–12, Nov. 2026, [Online]. Available: https://izlik.org/JA66CZ85NZ

ISNAD

Adil, Mir Muhammad. “CRISPR/Cas12a-Based/Biosensors/for/Rapid/Detection/of/Carbapenem/Resistance/Genes:/Recent/Advances/and/Point-of-Care/Prospects”. Turkish Journal of Sensors and Biosensors. 2026-1 (November 15, 2026): 1-12. https://izlik.org/JA66CZ85NZ.

JAMA

1.Adil MM. CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects. Turkish Journal of Sensors and Biosensors. 2026;:1–12.

MLA

Adil, Mir Muhammad. “CRISPR/Cas12a-Based/Biosensors/for/Rapid/Detection/of/Carbapenem/Resistance/Genes:/Recent/Advances/and/Point-of-Care/Prospects”. Turkish Journal of Sensors and Biosensors, no. 2026-1, Nov. 2026, pp. 1-12, https://izlik.org/JA66CZ85NZ.

Vancouver

1.Mir Muhammad Adil. CRISPR/Cas12a-Based Biosensors for Rapid Detection of Carbapenem Resistance Genes: Recent Advances and Point-of-Care Prospects. Turkish Journal of Sensors and Biosensors [Internet]. 2026 Nov. 15;(2026-1):1-12. Available from: https://izlik.org/JA66CZ85NZ

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