Cydonia oblonga Yaprağının Meme Kanseri (MCF-7) ve Karaciğer Kanseri (HepG2) Hücre Hatları Üzerindeki Antioksidan, Antimikrobiyal ve Sitotoksik Aktivitesinin Araştırılması

Abstract

Dünyadaki en yaygın patolojilerden biri olan kanser, yüzyıllardır birçok insanın yaşam standardını düşürmekte ve ölüme neden olabilmektedir. Birçok şifalı bitki, hastalık semptomlarını iyileştirme yetenekleri ve düşük yan etkileri nedeniyle kanser tedavisinde yaygın olarak kullanılmaktadır. Şifalı bitkiler arasında yer alan ve sağlık açısından önemli özelliklere sahip olan Cydonia oblonga (C. oblonga) yaprağı, halk arasında ayva yaprağı olarak bilinen bir bitki türüdür. Asya kökenli olmasına rağmen günümüzde birçok ülkede yetiştirilmektedir. Bu çalışmada, C. oblonga yapraklarının MCF-7 ve HepG2 hücre hatları üzerindeki antioksidan, antimikrobiyal ve sitotoksik aktiviteleri araştırılmıştır. Yöntem olarak, toplanan C. oblonga yaprakları metanol ve hekzan çözücülerinde ekstrakte edilmiştir. Ekstraktlar MCF-7 ve HepG2 hücre hatları üzerinde sitotoksik aktivite için MTT Assay yöntemi, antioksidan aktivite için DPPH yöntemi ve ekstraktın beş farklı mikroorganizma üzerindeki antimikrobiyal etkileri için well agar yöntemi kullanılarak test edilmiştir. Sonuç olarak, C. oblonga yaprağı her iki hücre hattında da hekzan özütü metanol özütüne göre daha güçlü sitotoksik aktivite sergilemesine rağmen, metanol özütü hekzan özütüne göre daha güçlü antioksidan etkiye sahip bulunmuştur. Mikroorganizmalara uygulanan ekstraktlar en iyi zon çapı etkisini E. coli üzerinde, en düşük etkiyi ise B. megaterium üzerinde göstermiştir. Ayrıca, C. oblonga yaprağının antikanser ve antioksidan aktiviteden daha güçlü antimikrobiyal etkilere sahip olduğu gözlemlenmiştir.

Keywords

• Cydonia oblonga
• sitotoksik aktivite
• antioksidan aktivite
• antimikrobiyal aktivite

Investigation of Antioxidant, Antimicrobial and Cytotoxic Activity of Cydonia oblonga Leaf on Breast Cancer (MCF-7) and Liver Cancer (HepG2) Cell Lines

Abstract

Cancer, one of the most common pathologies in the world, has been reducing the standard of living of many people for centuries and can cause death. Many medicinal plants are widely used in cancer treatment due to their ability to improve disease symptoms and low side effects. Cydonia oblonga (C. oblonga) leaf, which is among the medicinal plants and has important health properties, is a type of plant commonly known as quince leaf. Although it originates from Asia, it is cultivated in many countries today. In this study, antioxidant, antimicrobial and cytotoxic activities of C. oblonga leaves on MCF-7 and HepG2 cell lines were investigated. As a method, the collected C. oblonga leaves were extracted in methanol and hexane solvents. The extracts were tested on MCF-7 and HepG2 cell lines using MTT Assay method for cytotoxic activity, DPPH method for antioxidant activity and well agar method for antimicrobial effects of the extract on five different microorganisms. As a results, although C. oblonga leaf in both cell lines hexane extract exhibited stronger cytotoxic activity than methanol extract, methanol extract was found to have stronger antioxidant effect than hexane extract. The extracts applied to microorganisms showed the best zone diameter effect on E. coli and the lowest effect on B. megaterium. In addition, it was observed that C. oblonga leaf has stronger antimicrobial effects than anticancer and antioxidant activity.

Keywords

• Cydonia oblonga
• sitotoksik aktivite
• antioksidan aktivite
• antimikrobiyal aktivite

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