The Effect of Photoperiod on Growth, and Protein, Lipid and Chlorophyll Content in Scenedesmus Acutus

Yıl 2020, Cilt: 15 Sayı: 2, 79 - 84, 24.09.2020

Nur Agırman Kaymaz Ahmet Kadri Cetin

Öz

The effect of photoperiod on the growth, protein, lipid and pigment content in Scenedesmus acutus was examined on cultures exposed to 23 ± 1 °C for ten days at the light intensity of 55 µmol photon m-2s-1. Results showed that photoperiods had an effect on cell number, and protein and chlorophyll content. The growth, protein, lipid and chlorophyll levels in Scenedesmus acutus cultures reached maximum in different days and photoperiods. The number of cells of the continuously illuminated cultures was highest on day seven after inoculation. The total protein and total lipid content were highest on day eight under the light/dark photoperiod of 20/4 h while total chlorophyll content was highest on day ten under the light/dark photoperiod of 24:0 h. The cultures continuously illuminated for cell growth had the highest specific growth rate (µ=0.2304). Protein content had a specific growth rate of µ=0.0725 under the light/dark photoperiod of 20/4 h while total chlorophyll content had a specific growth rate of µ=0.0890 under the light/dark photoperiod of 12:12-h.

Anahtar Kelimeler

Green algae, Scenedesmus acutus, photoperiod, growth, lipid

Scenedesmus acutus’un Gelişim, Protein, Lipid ve Pigment Miktarı Üzerindeki Fotoperiyodun Etkisi

Öz

Fotoperiyodun Scenedesmus acutus’un gelişim, protein, lipid ve pigment miktarı üzerindeki etkileri, 55 µmolphoton m2s-1 ışık yoğunluğunda 23±1 °C sıcaklığa maruz bırakılan kültürlerde on gün süresince incelendi. Sonuçlar hücre sayısı, protein ve klorofil miktarı üzerinde fotoperiyodun etkili olduğunu gösterdi. Scenedesmus acutus kültürlerinde gelişme, protein, lipid ve klorofil miktarlarının farklı gün ve fotoperiyotta maksimuma ulaştığı tespit edildi. Sürekli aydınlatılan kültürlerde hücre sayısı inokülasyonu takip eden yedinci günde maksimuma ulaşırken, toplam protein ve total lipid miktarının 20:4-h aydınlık/karanlık fotoperiyodunda sekizinci günde, toplam klorofil miktarının da 24:0-h aydınlık/karanlık fotoperiyodunda onuncu günde maksimum olduğu görüldü. Spesifik büyüme oranı hücre gelişimi için sürekli aydınlatılan kültürlerde µ=0.2304 ile maksimum olurken protein miktarındaki spesifik büyüme oranı 20:4-h aydınlık/karanlık fotoperiyodunda µ=0.0725 ve toplam klorofil miktarındaki spesifik büyüme oranı ise 12:12-h aydınlık/karanlık fotoperiyodunda µ=0.0890 olduğu tespit edildi. 

Anahtar Kelimeler

Yeşil alg, Scenedesmus acutus, fotoperiyot, büyüme, lipit

Kaynakça

• Krzemińska I., Pawlik-Skowrońska B., Trzcinska M. and Tys J. (2014). Influence of photoperiods on the growth rate and biomass productivity of green microalgae. Bioprocess. Biosyst. Eng. 37: 735-741.
• Khoeyi ZA., Seyfabadi J. and Ramezanpour Z. (2012). Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris. Aquacult. Int. 20:41-49.
• Tzovenis I., Pauw ND. and Sorgeloos P. (1997). Effect of different light regimes on the docosahexaenoic acid (DHA) content of Isochrysis aff. galbana (clone T-ISO). Aquaculture International. 5: 489-507.
• Price LL., Yin K. and Harrison PJ. (1998). Influence of continuous light and L:D cycles on the growth and chemical composition of Prymnesiophyceae including coccolithophores. J Exp. Biol. Ecol. 223: 223-234.
• Fabregas J., Maseda A., Dominquez A., Ferreira M. and Otero A. (2002). Changes in the cell composition of the marine microalga, Nannochloropsis gaditana, during a light:dark cycle. Biotechnoll. Let. 24: 1699-1703.
• Wahidin S., Idris A. and Shaleh SRM. (2013). The influence of light intensity and photoperiod on the geowth and lipid content of microalgae Nannochloropsis sp. Bioresour. Technol. 129: 7-11.
• Richmond A. (2004). Biological principles of mass cultivation. In Richmond A (ed) Handbook of microalgal mass, culture: biotechnology and applied phycology. CRC Press, Blackwell Publishing Company, Oxford, pp 566.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol regent. J. Biol. Chem. 1951; 193:265-75.
• Bligh EG, Dyer WJ. A rapid method for total lipid extraction and purification. Can. Biochem. Physiol. 1959; 37:911-917.
• Strickland J. and Pearson TR. (1972). A Pratical Handbook of Seawater Analysis, second ed. Fish Res. Can. Bull.
• Renaud SM., Parry DL., Thinh LV, Kuo C., Padovan A. and Sammy N. (1991). Effect of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp. and Nannochloropsisi oculata for use in tropical aquaculture. J. Appl. Phycol. 3: 43-53.
• Sanchez-Saavedra MP. and Voltolina D. (2002). Effect of photon fulence rates of hite and blue-green light on growth efficiency and pigment content of three diatom species in batch cultures. Cienc. Mar. 28: (3): 273-279.
• Seyfabadi J. Ramezanpour Z. and Khoeyi ZA. (2011). Protein, fatty acid, and pigment content of Chlorella vulgaris under different light regimes. J Appl. Phycol. 23: 721-726.
• Bouterfas R., Belkoura M. and Dauta A. (2006). The effcts of irradiance and photoperiod on the growth rate of three freshwater green lgae isolated from a eutrophic lake. Limnetica, 25 (3): 647-656.
• Watson D., Daume S., Prince J., Beazley L. and Knott B. (2004). The influence of light intensity on the density on the density of different diatoms as fed for juvenile greenlip abalone (Haliotis laevigata). Aquaculture, 235: 345-359.
• Meseck SL., Alix JH., Gary H. and Wikfors GH. (2005). Photoperiod and light intensity effects on growth and utilization of nutrients by the aquaculture feed microalga Tetraselmis chui (Ply429). Aquaculture, 246: 393-404.
• Oh SH., Han JG., Kim Y Ha JH., Kim SS., Jeong MH., Jeong HS., NY., Cho JS., Yoon WB., Lee SY., Kang DH. and Lee HY. (2009). Lipid production in Porphyridium cruentum grown under different culture conditions. Journal of Bioscience and Bioenginering, 108, 5, 429-434.
• Ruangsomboon S. (2012). Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2. Bioresource Technology, 109: 261-265.
• Mata TM., Melo AC., Simoes M. and Caetano NS. (2012). Parametric study of a brewery effluent treatment by microalgae Scenedesmus obliquus. Bioresour. Technol. 107: 151-158.
• Kotimchenko SV. and Yakovleva IM. (2005). Lipid composition of the red alga Tichocarpus crinitus exposed to different levels of photon irradiance. Phytochemistry, 66: 73-79.
• Singh SP. and Singh P. (2015). Effect of temperature and light on the growth of algae species: A review. Renewable and Sustinable Energy Reviews, 50: 431-444.