The Effect of Photoperiod on Growth, and Protein, Lipid and Chlorophyll Content in Scenedesmus Acutus

Year 2020, Volume: 15 Issue: 2, 79 - 84, 24.09.2020

Nur Agırman Kaymaz Ahmet Kadri Cetin

Abstract

The effect of photoperiod on the growth, protein, lipid and pigment content in Scenedesmus acutus was examined on cultures exposed to 23 ± 1 °C for ten days at the light intensity of 55 µmol photon m-2s-1. Results showed that photoperiods had an effect on cell number, and protein and chlorophyll content. The growth, protein, lipid and chlorophyll levels in Scenedesmus acutus cultures reached maximum in different days and photoperiods. The number of cells of the continuously illuminated cultures was highest on day seven after inoculation. The total protein and total lipid content were highest on day eight under the light/dark photoperiod of 20/4 h while total chlorophyll content was highest on day ten under the light/dark photoperiod of 24:0 h. The cultures continuously illuminated for cell growth had the highest specific growth rate (µ=0.2304). Protein content had a specific growth rate of µ=0.0725 under the light/dark photoperiod of 20/4 h while total chlorophyll content had a specific growth rate of µ=0.0890 under the light/dark photoperiod of 12:12-h.

Keywords

Green algae, Scenedesmus acutus, photoperiod, growth, lipid

Scenedesmus acutus’un Gelişim, Protein, Lipid ve Pigment Miktarı Üzerindeki Fotoperiyodun Etkisi

Abstract

Fotoperiyodun Scenedesmus acutus’un gelişim, protein, lipid ve pigment miktarı üzerindeki etkileri, 55 µmolphoton m2s-1 ışık yoğunluğunda 23±1 °C sıcaklığa maruz bırakılan kültürlerde on gün süresince incelendi. Sonuçlar hücre sayısı, protein ve klorofil miktarı üzerinde fotoperiyodun etkili olduğunu gösterdi. Scenedesmus acutus kültürlerinde gelişme, protein, lipid ve klorofil miktarlarının farklı gün ve fotoperiyotta maksimuma ulaştığı tespit edildi. Sürekli aydınlatılan kültürlerde hücre sayısı inokülasyonu takip eden yedinci günde maksimuma ulaşırken, toplam protein ve total lipid miktarının 20:4-h aydınlık/karanlık fotoperiyodunda sekizinci günde, toplam klorofil miktarının da 24:0-h aydınlık/karanlık fotoperiyodunda onuncu günde maksimum olduğu görüldü. Spesifik büyüme oranı hücre gelişimi için sürekli aydınlatılan kültürlerde µ=0.2304 ile maksimum olurken protein miktarındaki spesifik büyüme oranı 20:4-h aydınlık/karanlık fotoperiyodunda µ=0.0725 ve toplam klorofil miktarındaki spesifik büyüme oranı ise 12:12-h aydınlık/karanlık fotoperiyodunda µ=0.0890 olduğu tespit edildi. 

Keywords

Yeşil alg, Scenedesmus acutus, fotoperiyot, büyüme, lipit

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