The Antioxidant Potential Role of Mate Leaf (Ilex paraguariensis) Extract on Saccharomyces cerevisiae

Year 2026, Volume: 21 Issue: 1, 1 - 13

Ceren Güneş , Zeynep Buldum , Seda Beyaz , İrem Nur Gözel , Abdullah Aslan

https://doi.org/10.55525/tjst.1675600

Abstract

Mate leaf (Ilex paraguariensis), a medicinal plant rich in polyphenolic compounds, has been found to possess various biological activities, including antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant, anticancer, anti-cholesterol, antidiabetic, and antihypertensive effects. This study investigates the protective and preventive effects of Mate leaf extract on oxidative damage induced by potassium dichromate (K2Cr2O7) in Saccharomyces cerevisiae. Four groups were created for the study: (i) Control Group: The group created with yeast only; (ii) Mate Leaf Group: The group receiving Mate leaf extract (10%); (iii) K2Cr2O7 Group: The group receiving K2Cr2O7 (10 millimolar); (iv) Mate Leaf + K2Cr2O7 Group: The group receiving both Mate leaf (10%) and K2Cr2O7 (10 millimolar). Cell development time (1, 3, 5, 24 hours), lipid peroxidation, malondialdehyde (MDA) analysis, catalase (CAT) activity, and glutathione (GSH) levels were measured using a spectrophotometer. Total protein changes in S. cerevisiae cultures were determined at 1, 3, 5, and 24 hours using SDS-PAGE electrophoresis and calculated by the Bradford method. Results revealed that Mate leaf extract had protective and preventive effects against oxidative stress caused by K2Cr2O7 in S. cerevisiae. Data showed that Mate leaf improved biochemical parameters (GSH, CAT) in cells against K2Cr2O7-induced oxidative damage. Specifically, the Mate leaf-treated group exhibited decreased lipid peroxidation levels, increased catalase (CAT) activity, and elevated glutathione (GSH) levels. Moreover, analyses of cell development time and total protein levels indicated that Mate leaf treatment protected cell health. These findings support the potential of Mate leaf as a protective agent against oxidative damage and suggest that its biological activity could be beneficial.

Keywords

Ilex paraguariensis , GSH , Potasyum dikromat , SDS-PAGE.

Mate Yaprağı (Ilex paraguariensis) Ekstraktının Saccharomyces cerevisiae Üzerinde Antioksidan Potansiyel Rolü

Abstract

Mate yaprağı (Ilex paraguariensis), polifenolik bileşikler bakımından zengin olan ve antimikrobiyal, antifungal, antiviral, antienflamatuar, antioksidan, antikanser, anti-kolesterol, antidiyabetik ve antihipertansif etkiler gibi çeşitli biyolojik aktivitelere sahip olduğu bilinen tıbbi bir bitkidir. Bu çalışma, potasyum dikromat (K₂Cr₂O₇) tarafından Saccharomyces cerevisiae’de oluşturulan oksidatif hasar üzerinde Mate yaprağı ekstraktının koruyucu ve önleyici etkilerini araştırmaktadır. Çalışma için dört grup oluşturulmuştur: (i) Kontrol Grubu: Sadece maya içeren grup; (ii) Mate Yaprağı Grubu: %10 Mate yaprağı ekstraktı verilen grup; (iii) K₂Cr₂O₇ Grubu: 10 milimolar K₂Cr₂O₇ verilen grup; (iv) Mate Yaprağı + K₂Cr₂O₇ Grubu: Hem %10 Mate yaprağı ekstraktı hem de 10 milimolar K₂Cr₂O₇ verilen grup. Hücre gelişim süresi (1, 3, 5, 24 saat), lipid peroksidasyonu, malondialdehit (MDA) analizi, katalaz (CAT) aktivitesi ve glutatyon (GSH) düzeyleri spektrofotometre kullanılarak ölçülmüştür. S. cerevisiae kültürlerinde toplam protein değişiklikleri 1, 3, 5 ve 24. saatlerde SDS-PAGE elektroforezi yöntemiyle belirlenmiş ve Bradford yöntemiyle hesaplanmıştır. Mate yaprağı ekstraktı K₂Cr₂O₇’nin neden olduğu oksidatif strese karşı koruyucu ve önleyici etkilere sahip olduğunu ortaya koymuştur. Veriler, Mate yaprağının K₂Cr₂O₇ kaynaklı oksidatif hasara karşı hücrelerdeki biyokimyasal parametreleri (GSH, CAT) iyileştirdiğini göstermektedir. Özellikle Mate yaprağı ile muamele edilen grupta lipid peroksidasyon düzeylerinde azalma, katalaz (CAT) aktivitesinde artış ve glutatyon (GSH) seviyelerinde yükselme gözlemlenmiştir. Ayrıca hücre gelişim süresi ve toplam protein düzeyleri analizleri, Mate yaprağı uygulamasının hücre sağlığını koruduğunu göstermiştir. Bu sonuçlar, mate yaprağının oksidatif hasara karşı koruyucu bir ajan olarak potansiyelini desteklemekte ve biyolojik aktivitesinin faydalı olabileceğini düşündürmektedir.

Keywords

Ilex paraguariensis , GSH , Potasyum dikromat , SDS-PAGE

References

• Cheng G, Yan Y, Zheng B, Yan D. The Applications of Plant Polyphenols: Implications for the development and biotechnological utilization of Ilex species. Plants 2024; 13(23): 3271.
• Bastos DHM, Saldanha LA, Catharino RR, Sawaya AC, Cunha IB, Carvalho PO, and Eberlin MN. Phenolic antioxidants identified by ESI-MS from yerba mate (Ilex paraguariensis) and green tea (Camelia sinensis) extracts. Molecules 2007; 12(3):423-432.
• Gan RY, Zhang D, Wang M, and Corke H. Health benefits of bioactive compounds from the genus Ilex, a source of traditional caffeinated beverages. Nutrients 2018; 10(11):1682.
• Santos D, Frota EG, Vargas BK, Gris CCT, Dos Santos LF, Bertolin TE. What is the role of phenolic compounds of yerba mate (Ilex paraguariensis) in gut microbiota?. Phytochemistry 2022; 203:113341.
• Gerber T, Nunes A, Moreira BR, and Maraschin M. Yerba mate (Ilex paraguariensis A. St.‐Hil.) for new therapeutic and nutraceutical interventions: A review of patents issued in the last 20 years (2000–2020). Phytother Res 2023; 37(2):527-548.
• Bracesco N. Ilex paraguariensis as a healthy food supplement for the future world. Biomed J Sci Technol Res 2019; 16(1):11821-11823.
• Dabulici CM, Sârbu I, and Vamanu E. The bioactive potential of functional products and bioavailability of phenolic compounds. Foods 2020; 9(7):953.
• Aleksandrova K, Koelman L, and Rodrigues CE. Dietary patterns and biomarkers of oxidative stress and inflammation: A systematic review of observational and intervention studies. Redox Biol 2021; 42:101869.
• Liu H, Guan H, He F, Song Y, Li F, Sun-Waterhouse D, Li D. Therapeutic actions of tea phenolic compounds against oxidative stress and inflammation as central mediators in the development and progression of health problems: A review focusing on microRNA regulation. Crit Rev Food Sci Nutr 2024; 64(23):8414-8444.
• Parveen K, Khan MR, Siddiqui WA. Pycnogenol® prevents potassium dichromate (K2Cr2O7)-induced oxidative damage and nephrotoxicity in rats. Chem Biol Interact 2009; 181(3):343-350.
• Avila-Rojas SH, Tapia E, Briones-Herrera A, Aparicio-Trejo OE, León-Contreras JC, Hernández-Pando R, and Pedraza-Chaverri J. Curcumin prevents potassium dichromate (K2Cr2O7)-induced renal hypoxia. Food Chem Toxicol 2018; 121:472-482.
• Piovezan-Borges AC, Valério-Júnior C, Gonçalves IL, Mielniczki-Pereira AA, Valduga AT. Antioxidant potential of yerba mate (Ilex paraguariensis St. Hil.) extracts in Saccharomyces cerevisiae deficient in oxidant defense genes. Braz J Biol 2016; 76(2):539-544.
• Braconi D, Bernardini G, and Santucci A. Saccharomyces cerevisiae as a model in ecotoxicological studies: A post-genomics perspective. J Proteomics 2016; 137:19-34.
• Vanderwaeren L, Dok R, Voordeckers K, Nuyts S, Verstrepen KJ. Saccharomyces cerevisiae as a model system for eukaryotic cell biology, from cell cycle control to DNA damage response. Int J Mol Sci 2022; 23(19):11665.
• Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2):351-358.
• Hossam El Din HA, AbdAllah AA, Afify MM, Mahmoud NF, Guo J, Murad SA, Ibrahim EA. Protective action of polysaccharides from Laurencia papillose (Rhodophyta) against imidacloprid-induced genotoxicity and oxidative stress in male albino rats. Environ Anal Health Toxicol 2022; 37(2):e2022011.
• Elman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959; 82(1):70-77.
• Dag M. Comparison of malondialdehyde and reduced glutathione in some rat tissues in hypoxia and obesity. Phoenix Med J 2022; 4(2):67-71.
• Aydinoglu F, and Akgul B. Investigation of microRNA-mediated redox regulation in leaf growth regions during freeze stress tolerance of maize (Zea mays L.). Anatol J Agric Sci 2019; 34(2):172-183.
• Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254.
• Nergiz MA, Beyaz S, Gok O, and Aslan A. Evaluation of the antioxidative effects of Hibiscus sabdariffa L. extract on Saccharomyces cerevisiae with molecular biological and biochemical biomarkers. J Appl Biol Sci 2024; 18(3):342-354.
• Beyaz S. Investigation of antioxidant activities of Melissa officinalis and Lavandula angustifolia extracts against chromium-induced oxidative damage in Saccharomyces cerevisiae with molecular biological and biochemical biomarkers. Prog Nutr 2022; 24(3):2-11.
• Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227(5259):680–685.
• Aslan A. Protective effects of goji berry on chromium (K2Cr2O7)-induced oxidative damage in Saccharomyces cerevisiae. Bitlis Eren Univ J Sci Technol 2021; 10:784-795.
• Heck CI, De Mejia EG. Yerba Mate Tea (Ilex paraguariensis): A comprehensive review on chemistry, health implications, and technological considerations. J Food Sci 2007; 72(9):R138-R151.
• Mellouk Z, Idrissi TH, and Kruyt J. Protective effects of orange juice extract on chromium-induced oxidative damage in Saccharomyces cerevisiae. J Pharm Biol Sci 2019; 11(3):19-23.
• Muccillo-Baisch AL, Rafael de Moura F, Penteado JO, Fernandes CLF, Costa Bueno E, Menestrino Garcia E, Silva Júnior FMR. Beneficial effects of mate-herb, Ilex paraguariensis St. Hil. against potassium dichromate-induced oxidative stress and nephrotoxicity. J Toxicol Environ Health A 2023; 86(13):446-457.
• Colpo AC, de Lima ME, Maya-López M, Rosa H, Márquez-Curiel C, Galván-Arzate S, Santamaria A, and Folmer V. Compounds from Ilex paraguariensis extracts have antioxidant effects in the brains of rats subjected to chronic immobilization stress. Appl Physiol Nutr Metab 2017; 42(11):1172-1178.
• Camara FD, Pedroso GS, Roman SS, Dallago RM, Valduga AT, Fernandes BB, Cunha EBB, Silveira PCL et al. Yerba mate (Ilex paraguariensis St. Hil.) extract inhibits hand-rolled corn-husk cigarette smoke-induced oxidative pulmonary damage. Anais Acad Bras Cienc 2020; 92:e20191141.
• Paluch E, Okińczyc P, Zwyrzykowska-Wodzińska A, Szperlik J, Żarowska B, Duda-Madej A, Bąbelewski P, Włodarczyk M et al. Composition and antimicrobial activity of Ilex leaves water extracts. Molecules 2021; 26(24):7442-7477.
• Bashandy SA, Ebaid H, Al-Tamimi J, Ahmed-Farid OAH, Omara EA, and Alhazza IM. Melatonin alleviated potassium dichromate‐induced oxidative stress and reprotoxicity in male rats. Biomed Res Int 2021; 2021:3565360.
• Ullah A, Munir S, Badshah SL, Khan N, Ghani MA, Poulson PR, Emwas AH. Important flavonoids and their role as a therapeutic agent. Molecules 2020; 25(22):5243.
• Beyaz S, Gok O, and Aslan A. The determination of the effect of Curcumin on Saccharomyces cerevisiae total protein expression changes and cell growth. Prog Nutr 2021; 23(1):1-10.
• Raj A, and Nachiappan V. Exposure to benzene metabolites causes oxidative damage in Saccharomyces cerevisiae. Antonie van Leeuwenhoek 2016; 109:841-854.
• Bernardi A, Ballestero P, Schenk M, Ferrario M, Gómez G, Rivero R, Avale E, Taravini I, Gershanik O, Guerrero S, and Ferrario JE. Yerba mate (Ilex paraguariensis) favors survival and growth of dopaminergic neurons in culture. Mov Disord 2019; 34(6):920-922.
• Beyaz S, Gök Ö, Aslan A. The therapeutic effects and antioxidant properties of epigallocatechin-3 gallate: A new review. IJSM 2022; 9(2): 125-136.
• Aslan A. Cell culture developing and the imaging of total protein product changing with SDS-PAGE in Saccharomyces cerevisiae. Prog Nutr 2018; 20(1): 128-132.