The effect of intra articular application of adipose tissue-derived mesenchymal stem cells on the clinical complaints in two cats with osteochondrodysplasia

Year 2025, Volume: 9 Issue: 2, 137 - 142, 30.10.2025

Yasin Parlatır , Yalçın Alper Özturan , İbrahim Süner , Burak Antakyalıoğlu , Mümin Alper Erdoğan

https://doi.org/10.47748/tjvr.1616194

Abstract

Osteochondrodysplasia is a degenerative joint condition caused by a TRPV4 gene mutation, predominantly affecting Scottish Fold cats. This study evaluated the effects of adipose-derived mesenchymal stem cells (MSCs) in two Scottish Fold cats diagnosed with osteochondrodysplasia. Case 1 involved a 3-year-old cat with a one-month history of lameness and joint degeneration, while Case 2 featured a 4-year-old cat with a five-month history of similar symptoms. MSCs were isolated, characterized at passage 3, and administered intra-articularly at a dose of 1 × 10^6 cells/kg body weight. Both cats demonstrated significant clinical improvement, including resolution of lameness and increased mobility, despite persistent radiographic abnormalities. These findings suggest that MSC therapy may effectively alleviate clinical symptoms of osteochondrodysplasia. Further research is needed to explore its long-term efficacy and correlation with imaging outcomes.

Keywords

Osteochondrodisplazi , cat , stem cell , adipose , mesenchimal

Ethical Statement

ETHICAL STATEMENT The cat’s owners were provided with comprehensive verbal and written information regarding the proposed intervention, and the owner’s informed consent was duly obtained. Ethics committee approval for the current case report was submitted to the Kirikkale University Animal Experiments Ethics Committee, and it was confirmed that no ethics committee permission is required

Supporting Institution

This case report was supported by Adnan Menderes University techocity firm Chiron Pharma Mikrostem company in stem cell supply. The content is solely the authors' responsibility and does not necessarily represent the official views of the Mikrostem company

References

• Allan GS. Radiographic features of feline joint disease. Vet Clin North Am Small Anim Pract. 2000; 30:281-302.
• Bennett D, Zainal Ariffin SM, Johnston P. Osteoarthritis in the cat: how should it be managed and treated? J Fel Med Surg. 2012; 14(1):76-84.
• Black LL, Gaynor J, Gahring D, et al. Effect of adipose-derived mesenchymal stem and regenerative cells on lameness in dogs with chronic osteoarthritis of the coxofemoral joints: a randomized, double-blinded, multicenter, controlled trial. Vet Ther. 2007; 8:272-284.
• Bulk RL, Feeney DA. The Appendicular skeleton. In: Burk RL, Feeney DA, eds. Small Animal Radiology and Ultrasonography: A Diagnostic Atlas and Text. 3rd ed. Philadelphia: WB Saunders; 2003. p.543.
• Caplan AI. Mesenchymal stem cells: Time to change the name!. Stem Cells Transl Med. 2017; 6(6):1445-1451.
• Chang J, Jung J, Oh S, et al. Osteochondrodysplasia in three Scottish Fold cats. J Vet Sci. 2007; 8:307-309.
• Choudhary RK. Overview of stem cells and their applications in veterinary medicine. In: Choudhary RK, Choudhary S, eds. Stem Cells in Veterinary Science. Singapore: Springer; 2021. p.3-23.
• Drazilov SS, Mrkovacki J, Spasovski V, et al. The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis. Acta Vet Hung. 2018; 66:376-89.
• Fujiwara-Igarashi A, Igarashi H, Hasegawa D, et al. Efficacy and complications of palliative irradiation in three Scottish Fold cats with osteochondrodysplasia. J Vet Intern Med. 2015; 29:1643-1647.
• Gandolfi B, Alamri S, Darby WG, et al. A dominant TRPV4 variant underlies osteochondrodysplasia in Scottish fold cats. Osteoarthr Cartil. 2016; 24:1441-1450.
• Golemović M, Škifić M, Golubić Ćepulić B. Mesenchymal stem cells: immunomodulatory properties and clinical application. Liječnički Vjesnik. 2012; 134(1-2).
• Guillot M, Moreau M, d’Anjou M-A, et al. Evaluation of osteoarthritis in cats: Novel information from a pilot study. Vet Surg. 2012; 41(3):328-35.
• Lascelles BD, Robertson SA. DJD-associated pain in cats. What can we do to promote patient comfort? J Feline Med Surg. 2010; 12(3):200-212.
• Malik R, Allan GS, Howlett CR, et al. Osteochondrodysplasia in Scottish Fold cats. Aust Vet J. 1999; 77(2):85-93.
• Mathews KG, Koblik PD, Knoeckel MJ, et al. Resolution of lameness associated with Scottish Fold osteodystrophy following bilateral osteotomies and pantarsal arthrodesis:a case report. J Am Anim Hosp Assoc. 1995; 31:280-288.
• McKellar QA, May SA, Lees P. Pharmacology and therapeutics of non-steroidal anti-inflammatory drugs in the dog and cat: 2. Individual agents. J Small Anim Pract. 1991; 32:225-235.
• Mohoric L, Zorko B, Ceh K, et al. Blinded placebo study of bilateral osteoarthritis treatment using adipose derived mesenchymal stem cells. Slovenian Vet Res. 2016; 53:167-174.
• Nakajo T, Fujita Y, Ichinohe T, et al. Combined surgical, radiation, and medical therapies for Osteochondrodysplasia in a Scottish Fold Cat. J Am Anim Hosp Assoc. 2020; 56(3):175.
• Pedersen NC, Morgan JP, Vasseur PB. Joint diseases of dogs and cats. In: Ettinger SJ, Feldman EC, eds. Textbook of Veterinary Internal Medicine. 5th ed. Philadelphia: WB Saunders; 2000. p.1874.
• Quimby JM, Webb TL, Habenicht LM, et al. Safety and efficacy of intravenous infusion of allogeneic cryopreserved mesenchymal stem cells for treatment of chronic kidney disease in cats: results of three sequential pilot studies. Stem Cell Res Ther 2013; 4: 48.
• Rorden C, Griswold MC, Moses N, et al. Radiographical survey of osteochondrodysplasia in Scottish Fold cats caused by the TRPV4 gene variant. Hum Genet. 2021; 140:1525-1534.
• Takanosu M, Hattori Y. Osteochondrodysplasia in Scottish Fold cross-breed cats. J Vet Med Sci. 2020:82(12);1769-1772.
• Taylor PM, Delatour P, Landoni FM, et al. Pharmacodynamics and enantioselective pharmacokinetics of carprofen in the cat. Res Vet Sci. 1996; 60:144-161.
• Velie BD, Milden T, Miller H, et al. An estimation of osteochondrodysplasia prevalence in Australian Scottish Fold cats: a retrospective study using Vet Compass Data. BMC Vet Res. 2023; 19:252.
• Via AG, Frizziero A, Oliva F. Biological properties of mesenchymal stem cells from different sources. MLTJ. 2012; 2(3):154-162.
• Vilar JM, Morales M, Santana A, et al. Controlled, blinded force platform analysis of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells associated to PRGF-Endoret in osteoarthritic dogs. BMC Vet Res. 2013; 9:131.
• Voga M, Adamic N, Vengust M, et al. Stem cells in veterinary medicine-current state and treatment options. Front Vet Sci. 2020; 7:278.
• Webb TL, Quimby JM, Dow SW. In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells. J Feline Med Surg. 2012; 14:165-168.
• Wisner ER, Konde LJ. Diseases of the immature skeleton. In: Thrall DE, ed. Textbook of Veterinary Diagnostic Radiology. 4th ed. Philadelphia: WB Saunders; 2002. p.155-156.