The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells

Elif Ece Akgün; Esra Bilici

doi:10.47748/tjvr.1744060

The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells

Abstract

Objective: Synovial fluid-derived mesenchymal stem cells represent a minimally invasive and highly proliferative source of multipotent cells with emerging potential in regenerative medicine. While their mesodermal differentiation is established, limited data exist on their neurogenic differentiation potential in rabbits. This study aimed to isolate and characterize rabbit synovial fluid mesenchymal stem cells and assess their capacity for neurogenic differentiation in vitro. Materials and Methods: Cells were isolated from rabbit knee synovial fluid, cultured, and confirmed to express typical mesenchymal stem cell surface markers. After neurogenic differentiation process, cells also were confirmed with ENOS2, MAP2 and Nestin markers. Results: Following neurogenic induction, synovial fluid mesenchymal stem cells displayed neuron-like morphology and upregulated neural markers, including Nestin, ENO2, and MAP2, as confirmed by Real Time-PCR. For mesenchymal stem cell identification, compare to control group CD 44, CD 73, CD90 and CD 105 genes were upregulated while CD 34 and CD 45 genes were downregulated. Conclusion: These results demonstrate the neurogenic potential of rabbit synovial fluid mesenchymal stem cells and support their application in preclinical models for neural repair.

Keywords

Supporting Institution

The authors declared that this study has received no financial support

Ethical Statement

The ethical guidelines was approved by the Burdur Mehmet Akif Ersoy University Animal Ethic Committee (approval no. 1525, dated 9 July 2025).

References

Abeysinghe HC, Bokhari L, Quigley A, et al. Pre-differentiation of human neural stem cells into GABAergic neurons prior to transplant results in greater repopulation of the damaged brain and accelerates functional recovery after transient ischemic stroke. Stem Cell Res Ther. 2015;6:186.
Akgün EE, Demirtaş E, Özden Akkaya Ö, Erdoğan M, Altunbaş K. Effect of chondrogenic differentiation medium supplemented with BMP-9 and TGF-ß3 on hypertrophy in transwell co-culture. Kocatepe Vet J. 2022; 15(2):217-222.
Bami M, Sarlikiotis T, Milonaki M, et al. Superiority of synovial membrane mesenchymal stem cells in chondrogenesis, osteogenesis, myogenesis and tenogenesis in a rabbit model. Injury. 2020; 51(12):2855-2865.
Bernal A, Arranz L. Nestin-expressing progenitor cells: function, identity and therapeutic implications. Cell Mol Life Sci. 2018; 75(12):2177-2195.
Chijimatsu R, Miwa S, Okamura G, et al. Divergence in chondrogenic potential between in vitro and in vivo of adipose- and synovial-stem cells from mouse and human [published correction appears in Stem Cell Res Ther. 2021; 12(1):479.
Harris VK, Yan QJ, Vyshkina T, Sahabi S, Liu X, Sadiq SA. Clinical and pathological effects of intrathecal injection of mesenchymal stem cell-derived neural progenitors in an experimental model of multiple sclerosis. J Neurol Sci. 2012; 313(1-2):167-177.
Jia Z, Liu Q, Liang Y, et al. Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells. J Transl Med. 2018; 16(1):123.
Kim SU, de Vellis J. Stem cell-based cell therapy in neurological diseases: a review. J Neurosci Res. 2009; 87(10):2183-2200.

Lee DH, Sonn CH, Han SB, Oh Y, Lee KM, Lee SH. Synovial fluid CD34⁻ CD44⁺ CD90⁺ mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis. Osteoarthritis Cartilage. 2012; 20(2):106-109.
Lee WJ, Hah YS, Ock SA, et al. Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs. Exp Cell Res. 2015; 333(2):273-288.
Luo L, Urata Y, Yan C, et al. Radiation exposure decreases the quantity and quality of cardiac stem cells in mice. PloS One. 2016; 11(5):e0152179.
Marcus AJ, Coyne TM, Rauch J, Woodbury D, Black IB. Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane. Differentiation. 2008; 76(2):130-144.
Murata D, Miyakoshi D, Hatazoe T, et al. Multipotency of equine mesenchymal stem cells derived from synovial fluid. Vet J. 2014; 202(1):53-61.
Okolicsanyi RK, Camilleri ET, Oikari LE, et al. Human mesenchymal stem cells retain multilineage differentiation capacity including neural marker expression after extended in vitro expansion. PLoS One. 2015; 10(9):e0137255.
Palmer TD, Markakis EA, Willhoite AR, Safar F, Gage FH. Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS. J Neurosci. 1999; 19(19):8487-8497.
Rastegar F, Shenaq D, Huang J, et al. Mesenchymal stem cells: Molecular characteristics and clinical applications. World J Stem Cells. 2010; 2(4):67-80.
Sakaguchi Y, Sekiya I, Yagishita K, Muneta T. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum. 2005; 52(8):2521-2529.
Scuteri A, Miloso M, Foudah D, Orciani M, Cavaletti G, Tredici G. Mesenchymal stem cells neuronal differentiation ability: a real perspective for nervous system repair?. Curr Stem Cell Res Ther. 2011; 6(2):82-92.
Squillaro T, Peluso G, Galderisi U. Clinical trials with mesenchymal stem cells: An Update. Cell Transplant. 2016; 25(5):829-848.
Tan SL, Ahmad TS, Selvaratnam L, Kamarul T. Isolation, characterization and the multi-lineage differentiation potential of rabbit bone marrow-derived mesenchymal stem cells. J Anat. 2013; 222(4):437-450.
Tirpáková M, Vašíček J, Svoradová A, et al. Phenotypical characterization and neurogenic differentiation of rabbit adipose tissue-derived mesenchymal stem cells. Genes (Basel). 2021; 12(3):431.
Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells - current trends and future prospective. Biosci Rep. 2015; 35(2):e00191.
van Velthoven CT, Sheldon RA, Kavelaars A, et al. Mesenchymal stem cell transplantation attenuates brain injury after neonatal stroke. Stroke. 2013; 44(5):1426-1432.
Vašíček J, Kováč M, Baláži A, et al. Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. N Biotechnol. 2020; 54:1-12.
Wang N, Xu Y, Qin T, et al. Myocardin-related transcription factor-A is a key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells. Gene. 2013; 523(2):178-186.
Woodbury D, Reynolds K, Black IB. Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. J Neurosci Res. 2002; 69(6):908-917.
Zhang R, Li J, Li J, Xie J. Efficient In vitro labeling rabbit bone marrow-derived mesenchymal stem cells with SPIO and differentiating into neural-like cells. Mol Cells. 2014; 37(9):650-655.
Ziegler L, Grigoryan S, Yang IH, Thakor NV, Goldstein RS. Efficient generation of schwann cells from human embryonic stem cell-derived neurospheres. Stem Cell Rev Rep. 2011; 7(2):394-403.

Details

Primary Language

English

Subjects

Veterinary Histology and Embryology, Animal Science, Genetics and Biostatistics

Journal Section

Research Article

Authors

Elif Ece Akgün ^*
0000-0002-2982-0307
Türkiye

Esra Bilici
0000-0001-6636-5975
Türkiye

Early Pub Date

October 26, 2025

Publication Date

October 30, 2025

Submission Date

July 16, 2025

Acceptance Date

August 25, 2025

Published in Issue

Year 2025 Volume: 9 Number: 2

DOI

https://doi.org/10.47748/tjvr.1744060

IZ

https://izlik.org/JA92ZC46GJ

Cite

RIS / Bibtex

APA

Akgün, E. E., & Bilici, E. (2025). The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells. Turkish Journal of Veterinary Research, 9(2), 117-122. https://doi.org/10.47748/tjvr.1744060

AMA

1.Akgün EE, Bilici E. The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells. TJVR. 2025;9(2):117-122. doi:10.47748/tjvr.1744060

Chicago

Akgün, Elif Ece, and Esra Bilici. 2025. “The Neurogenic Differentiation of Rabbit Synovial Fluid Mesenchymal Stem Cells”. Turkish Journal of Veterinary Research 9 (2): 117-22. https://doi.org/10.47748/tjvr.1744060.

EndNote

Akgün EE, Bilici E (October 1, 2025) The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells. Turkish Journal of Veterinary Research 9 2 117–122.

IEEE

[1]E. E. Akgün and E. Bilici, “The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells”, TJVR, vol. 9, no. 2, pp. 117–122, Oct. 2025, doi: 10.47748/tjvr.1744060.

ISNAD

Akgün, Elif Ece - Bilici, Esra. “The Neurogenic Differentiation of Rabbit Synovial Fluid Mesenchymal Stem Cells”. Turkish Journal of Veterinary Research 9/2 (October 1, 2025): 117-122. https://doi.org/10.47748/tjvr.1744060.

JAMA

1.Akgün EE, Bilici E. The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells. TJVR. 2025;9:117–122.

MLA

Akgün, Elif Ece, and Esra Bilici. “The Neurogenic Differentiation of Rabbit Synovial Fluid Mesenchymal Stem Cells”. Turkish Journal of Veterinary Research, vol. 9, no. 2, Oct. 2025, pp. 117-22, doi:10.47748/tjvr.1744060.

Vancouver

1.Elif Ece Akgün, Esra Bilici. The neurogenic differentiation of rabbit synovial fluid mesenchymal stem cells. TJVR. 2025 Oct. 1;9(2):117-22. doi:10.47748/tjvr.1744060