Analysis of COI gene region of Varroa destructor in honey bees (Apis mellifera) in province of Siirt

Abstract

ABSTRACT

Objectives: Varroa destructor is the most damaging ectoparasite to the beekeeping economy. The mite has different haplotypes. It is aimed to determine which haplotype is present by examining the cytochrome c oxidase subunit 1 (COI) gene region of V. destructor found in honey bees in Siirt region.

Materials and Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) methods were applied in the analysis of the COI gene region of V. destructor in Siirt region. To do this, V. destructor samples were collected from 387 enterprises in the Siirt region. DNA extraction followed the PZR. Subsequently, 1.5% agarose gel images were obtained by electrophoresis. The PCR products were then subjected to XhoI and SacI restriction enzymes and 2% agarose gel images were obtained. 38 of the samples (10%) were sent to a private enterprise for sequencing. The obtained sequences were blasted and compared with the corresponding reference sequences in GenBank.

Results: According to the results of PZR and RFLP obtained from the 387 V. destructor samples in the study towards the COI gen region, all of the samples were found to be Korean haplotypes and Japanese haplotypes were not found in any of 387 samples. At the same time, it was also confirmed that the 38 sequenced samples were Korean haplotypes.

Discussion: The results obtained from this study are significant in terms of forming a groundwork for future studies.

Keywords

• PCR,Siirt,Varroa destructor

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