Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses

Abstract

Objective: In the this study, the effect of vitamin E plus selenium application on malondialdehyde, glutathion, vitamin E, C, A and b-karoten were aimed to investigate in exercised horses.

Material and Methods: For this purpose, 50 healtly Anatolian type local horse breed aged between 3-5 from Altındee Stud Farm were used. The animals were divided into two equal groups. While 1^st group received nothing, horses in second group were received vitamin E+selenium intramuscularly. Then animals in both groups were exercised for 1500 meters. Blood samples were taken handly from all animals before and after exercise. This samples were analysed for MDA, GSH, vitamin E, C, A and β-caroten spectrophotometrically.

Results: MDA and GSH concentration in 1^st group were found to increase significantly (p<0.001) after exercise. On the other hand, serum vitamin E, C, A and β-caroten levels did not changed significantly. In the second group, serum vitamin E levels increased significantly (p<0.001) after vitamin E+selenium application. Furthermore, MDA (p<0.05) and GSH (p<0.001) levels increased significantly after exercise in the second group.Vitamin E levels decreased significantly (p<0.01) after exercise. However, vitamin A and C levels did not change significantly. In addition, in the second group, β-caroten levels were also changed significantly (p<0.05) when the values obtained before vitamin E application compared with the values obtained after vitamin E application. When comparison made between groups, while MDA and vitamin E values were statistically important (p<0.05), GSH, vitamin C, A and β-caroten values were not important statistically

Conclusion: As a result, acute exercise can increase free-radical production which, the results shows that increase in both MDA and GSH can be shown as the indicator of  it. Furtermore, decrease in MDA level in vitamin E applied group the indicator of the rise in antioxidan defence and protective effect of vitamin E.

Keywords

• Antioxidant,horse,exercise,lipid peroxidation,vitamin E

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