Investigation of some biochemical and haematological parameters in sheep infected with Dicrocoelium dentriticum

Year 2020, Volume: 4 Issue: 1, 23 - 27, 10.03.2020

Vural Denizhan , Ayşe Karakuş

Abstract

Objective:The aim of this study was to investigate the changes in some biochemical and hematological parameters due to liver damage caused by parasites in sheep infected with Dicrocoelium (D.) dendriticum.
Material and Methods: The study was conducted on 10 healthy sheep and 60 sheep infected with D. dendriticum. After the blood was taken from the sheep brought to the slaughterhouse in Van Municipality, the liver and gall bladder were examined after the slaughter, and the sheep blood positive for D. dendriticum was brought to the laboratory and examined for some hematological and biochemical parameters.
Results: As a result of the examination, some changes were observed in the biochemical and hematological parameters. Statistical analysis: Hematologically compared with the healthy sheep group, of sheep infected with D. dentriticum; While WBC, Neu, Eo and Mon values were higher than healthy sheep group, RBC, Hct, Hb and MCV values were lower than healthy sheep group, there was no significant change in Lym level.
The results of biochemical parameters of D. dentriticum infected sheep were found to be higher according to the same parameters of the healty group sheep, while serum TP, ALB and glucose values were low.
Conclusion: As a result; WBC, Neu, Eo, Mon, ALT, AST and GGT levels in infected sheep due to dicrocoeliasis which cause important pathological disorders in liver organ that have functions such as synthesis, metabolism and detoxification in the organism were found to be significantly higher compared to the same values of the healthy group. It is predicted that these parameters may be useful in determining the degree and prognosis of liver damage and will shed light on the studies to be performed in this field.

Keywords

Dicrocoeliosis, Sheep, Biochemical, Hematologic

References

• Akyol VÇ. Bursa ortak girişim tesislerinde (Etba) kesilen koyunlarda Distamatosis’in yayılışı. J Fac Vet Med 2001; 20:23-27.
• Al-Hadithy HAH, Badawi NM, Mahmood MM. Estimation of serum liver enzymes activities in Awassis heep. Iraqi J Vet Med 2013; 37:115-120.
• Alvarez L, Moreno G, Moreno L, Ceballos L, Shaw L, Fairweather I, et al. Comparative assessment of albendazole and triclabendazole ovicidal activity on D. dentriticum eggs. Vet parasitol 2009; 164(2):211-216.
• Anderson PH, Berrett S, Brush PJ, Hebert CN, Parfitt JW, Patterson DSP. Biochemical indicators of liver injury in calves with experimental fasciolasis. Vet Rec 1977; 15:43-45.
• Baghshani H, Razmi GR, YaghfouriS, Dezaki AA. Investigation of selected biochemical parameters in sheep naturally infected with theileriosis. Comp Clin Path 2012; 21(6):1417-1420.
• Blood DC, Radostits OM, Arundel JH, Gay CC. Veterinary medicine. In: Diseases Caused by Helminth Parasites. 7th ed. London: Bailliere Tindall; 1989.
• Boch BJ, Supperer R. Veterinär medizinische Parasitologie. 5. Auflage. Berlin, Parey Buchverlag 2000; 353-354.
• Bosy-Westphal A, Petersen S, Hinrichsen H, Czech N, Müller MJ. Increased plasma homocysteine in liver cirrhosis. Hepatol Res 2001; 20:28-38.
• Calleja C, Bigot K, Eeckhoutte C, Sibille P, Boulard C, Galtier P. Comparison of hepatic and renal drug metabolising enzyme activities in sheep given single or two fold challenge infections with Fasciola hepatica. Int J Parasitol 2000; 30:953-958.
• Dağoğlu G, Değer S, Akgül Y, Aksoy A, Şekeroğlu R, Tarakçıoğlu R. Koyunlarda Doramectinin antiparaziter etkinliği ve serum enzimleri üzerine etkisi. Yüzüncü Yıl Üniv Sağ Bil Derg 1995; 1:1-5.
• Değer Y, Gül A, Bildik A, Dede S, Yur F, Değer S. Parazitli kopeklerin bazı kan parametreleri ile plazma vitamin C düzeylerinde görülen değişiklikler. Türkiye Parazitol Derg 1997; 21:195-198.
• Ducháček L, Lamka J. Dicrocoeliosis the presentstate of knowledge with respect to wild life species. Acta Vet Brno 2003; 72(4):613-626.
• Gaasenbeek CP, Moll L, Cornelissen JB, Vellema P, Borgsteede FH. An experimental study on triclabendazole resistance of Fasciola hepatica in sheep. Vet Parasitol 2001; 95:37-43.
• Gonzalez-Lanza C, Manga-Gonzalez MY, Compo R, Del-Pozo P, Sandoval H, Oleaga A, et al. IgG antibody responseto ES or somatic antigens of Dicrocoelium dendriticum (Trematoda) in experimentally infected sheep. Parasitol Res 2000; 86:472-479.
• Gonzalo-Orden M, Millan L, Alvarez M, Sanchez-Campos S, Jimenez R, Gonzalez-Gallego J, et al. Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings. Parasitol Res 2003; 90:359-364.
• Güralp N. Helmintoloji. İkinci baskı. Ankara: Ankara Üniversitesi Veteriner Fakültesi Yayınları; 1981.
• Jithendran KP, Bhat TK. Prevalence of dicrocoeliosis in sheepandgoats in HimachalPradesh. India. Vet Parasitol 1996; 61(3-4):265-271.
• Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals. 5th ed. San Diego: Academic; 1997.
• Kıladze M, Chipashvili L, Abuladze D, Jatchvliani D. Obstruction of common bile duct caused by liver fluke Fasciola hepatica. Sb Lek 2000; 101(3):255-259.
• Kozat S, Denizhan V. Glucose, lipid, and lipoprotein levels in sheep naturally infected with Fasciola hepatica. J Parasitol 2010; 96(3):657-659.
• Kozat S, Sepehrizadeh E. Methods of diagnosing in liver diseases of carnivores, A review. TJSR 2017; 10 (2):36-46.
• Kramer JW. Normal hematology of cattle, sheep and goats. In: Feldman BF, Zinkl JG, Jain NC (eds). Schalm’s Veterinary Hematology. 5th ed. Philadelphia: Lippincott Williams &Wilkins, 2000; 1075-1084.
• Manga-González MY, Ferreras MC. Dicrocoeliidae family: majör species causing veterinary diseases. Adv Exp Med Biol 2014; 766:393-428.
• Manga-González MY, Ferreras MC, Campo R, González-Lanza C, PérezV, García-Marín JF. Hepatic marker enzymes, biochemical parameters and pathological effects in lamb sex perimentally infected with Dicrocoelium dendriticum. Parasitol Res 2004; 93(5):344-355.
• Matanović K, Severin K, Martinkovic F, Simpraga M, Janicki Z, Barisic J. Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res 2007; 101:1657-1661.
• Otranto D, Traversa D. A review of dicrocoeliosis of ruminants including recent advances in the diagnosis and treatment. Vet Parasitol 2002; 107(4):317-335.
• Phiri IK, Phiri AM, Harrison LJS. The serum glucose and β-hydroxy butyrate levels in sheep with experimental Fasciola hepatica and Fasciola gigantica infection. Vet Parasitol 2007; 143(3):287-293.
• Raadsma HW, Kingsford NM, Suharyanta Spithill TW, Piedrafita D. Host responses during experimental infection with Fasciola gigantica and Fasciola hepatica in Merinos sheep. II. Development of a predictive index for Fasciola gigantica worm burden. Vet Parasitol 2008; 154:250-261.
• Sánchez -Campos S, Tunon MJ, Gonzales P, Gonzales-Gallego J. Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicrocoeliosis in hamsters. Parasitol Res 1999; 85:468-474.
• Severin K, Mašek T, Janicki Z, Konjević D, Slavica A, Marinculić A, et al. Liver enzyme sand blood metabolites in a population of free ranging red deer (Cervus elaphus) naturally infected with Fascioloides magna. J Helminthol 2012; 86(2):190-196.
• Sykes AR, Coop RL, Robinson MG. Chronic subclinic alovine fascioliasis: Plasma glutamate dehydrogenase, gamma-glutamyl liver lipids in fatty liver of dairy cows. ASJ 1980; 77:347-351.
• Şahin T, Akgül Y. Investigation of some trace element level sand biochemical parameters in sheep with endoparasite. YYU Sag Bil Derg 2006; 9:100-106.
• Şimşek S, Yuce A, Utuk AE. Determination of serum malondialdehyde levels in sheep naturally infected with Dicrocoelium dendriticum. FU Sağ Bil Derg 2006; 20:217-220.
• Thomas JS. Over view of plasma proteins. In: Feldman BF, Zinkl JG, Jain NC, (eds). Schalms Veterinary Hematology. Philadelphia: Lippincott Williams &Wilkins, 2000; 891-898.
• Toparlak M, Tüzer E. Paraziter Hastalıkların Tanısında Laboratuvar Teknikleri. İstanbul: İstanbul Üniversitesi Veteriner Fakültesi Parazitoloji Anabilim Dalı; 1994.
• Yadav CK, Sharma NN. Changes in blood chemical components during experimentally induced Theileria annulata infections in cattle. Vet Parasitol 1986; 21:91-98.
• Yang Q, Mao WH, Ferre I, Bayón JE, Mao XZ, González-Gallego J. Plasma aspartate amino transferase (AST), glutamate dehydrogenase (GLDH) and gamma-glutamyl transpeptidase (GGT) activities in water buffaloes with experimental subclinical fasciolosis. Vet Parasitol 1998; 78(2):129-136.
• Yuksek N, Altuğ N, Gul A. Therapeutic effect of the combination of trichlobendazole and levamisole in sheep with endoparasite infection. YYU Vet Fak Derg 2007; 18:19-24.