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Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Yıl 2020, Cilt 4, Sayı 2, 45 - 49, 30.10.2020

Öz

Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell. Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method. Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while it increased 7 times in the group treated with NaF+lycopene. A significant difference was detected between the groups in terms of gene expression pattern. While the Ripk 3 gene increased slightly in the 24th hour applied lycopene group, it was observed that only lycopene applied group increased 8 times and NaF+lycopene applied group increased in the 9 times Conclusion: Based on the results obtained from this study, it was seen that activation of necrotic genes is important in explaining the molecular basis of cell death from NaF, which is applied as fluorine source, in revealing the molecular basis of the necrotic pathway. It was found that the decrease in cell viability due to NaF increased with lycopene, but the use of lycopene with fluorine also increased necrotic gene expression. Keywords: NaF, in vitro, Lycopene, Necrotic Genes

Kaynakça

  • Adamek E, Pawłowska-Goral K, Bober K. In vitro and in vivo effects of fluoride ions on enzyme activity. Ann Acad Med Stetin 2005; 51(2): 69–85.
  • Agalakova NI, Gusev GP. Molecular mechanisms of cytotoxicity and apoptosis induced by inorganic fluoride. Cell Biology 2012, Article ID 403835.
  • Bramley PM. Is lycopene benefcial to human health? Phytochemistry 2000;54 (3): 233- 236.
  • Cao J, Chen J, Xie L, Wang J, Feng C, Song J. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway. Aquat Toxicol 2015;167,180-90.
  • Cetin S, Yur F, Taşpınar M, Dede S, Yüksek V. The effects of lycopene application on sodium fluoride (NaF) applied renal cell line. Int J Sec Metabolite 2017; 4 (3): 508-511.
  • Cetin S,Yur F, Taşpınar M, Yüksek V. The effect of some minerals on apoptosis and DNA damage in sodium fluoride-administered renal and osteoblast cell line. Fluoride 2019;52(3(Pt 2):362-378.
  • Cetin S, Değer Y, Dede S, Yur F. The concentration of certain trace elements in the wool of sheep with fluorosis. Fluoride 2020, 53(1 Pt 2):164-169.
  • Dharmaratne RW. Fluoride in drinking water and diet: the causative factor of chronic kidney diseases in the North Central Province of Sri Lanka. Environ Health Prev Med 2015;20(4):237-242.
  • Golstein P, Kroemer G. Cell death by necrosis: towards a molecular definition. Trends Biochem Sci 2007;32:37-43.
  • Hengartner MO, Ellis RE, Horvitz HR. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death. Nature 1992; 356: 494-499.
  • Karube H, Nishitai G, Inageda K, Kurosu H, Matsuoka M. NaF activates MAPKs and induces apoptosis in odontoblast-like cells. J Dent Res, 2009; 88(5):461–465.
  • Kuang P, Deng H, Liu H,et al. Sodium fluoride induces splenocyte autophagy via the mammalian targets of rapamycin (mTOR) signaling pathway in growing mice. Aging (Albany NY), 2018; 10(7):1649-1665.
  • Kubota K, Lee DH, Tsuchiya M, et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem 2005; 280 (24): 23194– 23202.
  • Lu J, Chen H, Xu Q, et al.Comparative proteomics of kidney samples from puffer fish Takifugu rubripes exposed to excessive fluoride: an insight into molecular response to fluorosis. Toxicol Mech Methods, 2010;20 (6):345–354.
  • Mashima R, Witting PK, Stocker R. Oxidants and antioxidants in atherosclerosis. Curr Opin Lipidol 2001; 12(4):411-418.
  • Mansour HH, Tawfik SS. Efficacy of lycopene against fluoride toxicity in rats. Pharm Biol 2012, 50(6):707-711.
  • Matos HR, Capelozzi VL, Gomes OF, Mascio PD, Medeiros MH. Lycopene inhibitis DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 2011, 396,171-174.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, et al . The effects of fluoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett 2009; 190(2):179–186.
  • Nicotera P, Bernassola F, Melino G. Regulation of the apoptosis-necrosis switch. Oncogene 2004;23:2757-2765.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. A brief review on experimental fluorosis. Toxicol Lett 2013; 223(2), 236-51.
  • Pruthi RS, Derksen E, Gaston K. Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors, a review. J Urol 2003; 169(6), 2352-2359.
  • Quadri JA, Alam MM, Sarwar S, Singh S, Shariff A, Das TK. Fluoride induced nephrotoxicity: apoptosis, ultra structural changes and renal tubular injury in experimental animals. Ayurveda and Pharma Res 2016;4(8):91-95.
  • Rousseau EJ, Davison AJ, Dunn B. Protection by beta-carotene and related compounds againist oxygen- mediated cytotoxicity and genotixicity implications for carcinogenesis and anticarcinogenesis. Free Radic Biol Med 1992; 13(4):407-433.
  • Rzeuski R, Chlubek D, Machoy Z. Interactions between fluoride and biological free radical reactions. Fluoride 1998(1): 43–44.
  • Shashi A, Singh JP, Thapar SP. Toxic effect of fluoride on rabbit kidney. Fluoride 2002; 35(1): 38–50.
  • Tan PP, Zhou BH, Zhao WP, Jia LS, Liu J, Wang HW. Mitochondria-mediated pathway regulates C2C12 cell apoptosis induced by fluoride. Biol Trace Elem Res 2018;185(2):440-447.
  • Tu W, Zhang Q, Liu Y, et al. Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells. Toxicol Appl Pharmacol 2018;347, 60-69.
  • Varol E, İcli A, Aksoy F, et al. Evaluation of total oxidative statusand total antioxidant capacity in patients with endemic fluorosis. Toxicol Ind Health 2013;29(2): 175–180.
  • Wang YY, Zhao BL, Li XJ, Su Z, Xi WJ. Spin trapping technique studies onactive oxygen radicals from human polymorphonuclear leukocytes during fluoride stimulated respiratory burst. Fluoride 1997;30(1):1-5.
  • Xiong X, Liu J, He W, et al. Dose effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children. Environm Res, 2007; 103, 112–116.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The effects of vitamins A, D, E, and C on apoptosis and DNA damage in sodium fluoride-treated renal and osteoblast cell lines. Fluoride 2017; 50(3):300-313.
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Jun signaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. Matrix Biol 2007;26 (8): 633–641.
  • Zhang M, Wang A, Xia T, He P. Effects of fluoride on DNA damage, S phase cell-cycle arrest and the expression of NF-kB in primary cultured rat hippocampal neurons. Toxicol Lett 2008;179(1):1–5.

Yıl 2020, Cilt 4, Sayı 2, 45 - 49, 30.10.2020

Öz

Kaynakça

  • Adamek E, Pawłowska-Goral K, Bober K. In vitro and in vivo effects of fluoride ions on enzyme activity. Ann Acad Med Stetin 2005; 51(2): 69–85.
  • Agalakova NI, Gusev GP. Molecular mechanisms of cytotoxicity and apoptosis induced by inorganic fluoride. Cell Biology 2012, Article ID 403835.
  • Bramley PM. Is lycopene benefcial to human health? Phytochemistry 2000;54 (3): 233- 236.
  • Cao J, Chen J, Xie L, Wang J, Feng C, Song J. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway. Aquat Toxicol 2015;167,180-90.
  • Cetin S, Yur F, Taşpınar M, Dede S, Yüksek V. The effects of lycopene application on sodium fluoride (NaF) applied renal cell line. Int J Sec Metabolite 2017; 4 (3): 508-511.
  • Cetin S,Yur F, Taşpınar M, Yüksek V. The effect of some minerals on apoptosis and DNA damage in sodium fluoride-administered renal and osteoblast cell line. Fluoride 2019;52(3(Pt 2):362-378.
  • Cetin S, Değer Y, Dede S, Yur F. The concentration of certain trace elements in the wool of sheep with fluorosis. Fluoride 2020, 53(1 Pt 2):164-169.
  • Dharmaratne RW. Fluoride in drinking water and diet: the causative factor of chronic kidney diseases in the North Central Province of Sri Lanka. Environ Health Prev Med 2015;20(4):237-242.
  • Golstein P, Kroemer G. Cell death by necrosis: towards a molecular definition. Trends Biochem Sci 2007;32:37-43.
  • Hengartner MO, Ellis RE, Horvitz HR. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death. Nature 1992; 356: 494-499.
  • Karube H, Nishitai G, Inageda K, Kurosu H, Matsuoka M. NaF activates MAPKs and induces apoptosis in odontoblast-like cells. J Dent Res, 2009; 88(5):461–465.
  • Kuang P, Deng H, Liu H,et al. Sodium fluoride induces splenocyte autophagy via the mammalian targets of rapamycin (mTOR) signaling pathway in growing mice. Aging (Albany NY), 2018; 10(7):1649-1665.
  • Kubota K, Lee DH, Tsuchiya M, et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem 2005; 280 (24): 23194– 23202.
  • Lu J, Chen H, Xu Q, et al.Comparative proteomics of kidney samples from puffer fish Takifugu rubripes exposed to excessive fluoride: an insight into molecular response to fluorosis. Toxicol Mech Methods, 2010;20 (6):345–354.
  • Mashima R, Witting PK, Stocker R. Oxidants and antioxidants in atherosclerosis. Curr Opin Lipidol 2001; 12(4):411-418.
  • Mansour HH, Tawfik SS. Efficacy of lycopene against fluoride toxicity in rats. Pharm Biol 2012, 50(6):707-711.
  • Matos HR, Capelozzi VL, Gomes OF, Mascio PD, Medeiros MH. Lycopene inhibitis DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 2011, 396,171-174.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, et al . The effects of fluoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett 2009; 190(2):179–186.
  • Nicotera P, Bernassola F, Melino G. Regulation of the apoptosis-necrosis switch. Oncogene 2004;23:2757-2765.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. A brief review on experimental fluorosis. Toxicol Lett 2013; 223(2), 236-51.
  • Pruthi RS, Derksen E, Gaston K. Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors, a review. J Urol 2003; 169(6), 2352-2359.
  • Quadri JA, Alam MM, Sarwar S, Singh S, Shariff A, Das TK. Fluoride induced nephrotoxicity: apoptosis, ultra structural changes and renal tubular injury in experimental animals. Ayurveda and Pharma Res 2016;4(8):91-95.
  • Rousseau EJ, Davison AJ, Dunn B. Protection by beta-carotene and related compounds againist oxygen- mediated cytotoxicity and genotixicity implications for carcinogenesis and anticarcinogenesis. Free Radic Biol Med 1992; 13(4):407-433.
  • Rzeuski R, Chlubek D, Machoy Z. Interactions between fluoride and biological free radical reactions. Fluoride 1998(1): 43–44.
  • Shashi A, Singh JP, Thapar SP. Toxic effect of fluoride on rabbit kidney. Fluoride 2002; 35(1): 38–50.
  • Tan PP, Zhou BH, Zhao WP, Jia LS, Liu J, Wang HW. Mitochondria-mediated pathway regulates C2C12 cell apoptosis induced by fluoride. Biol Trace Elem Res 2018;185(2):440-447.
  • Tu W, Zhang Q, Liu Y, et al. Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells. Toxicol Appl Pharmacol 2018;347, 60-69.
  • Varol E, İcli A, Aksoy F, et al. Evaluation of total oxidative statusand total antioxidant capacity in patients with endemic fluorosis. Toxicol Ind Health 2013;29(2): 175–180.
  • Wang YY, Zhao BL, Li XJ, Su Z, Xi WJ. Spin trapping technique studies onactive oxygen radicals from human polymorphonuclear leukocytes during fluoride stimulated respiratory burst. Fluoride 1997;30(1):1-5.
  • Xiong X, Liu J, He W, et al. Dose effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children. Environm Res, 2007; 103, 112–116.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The effects of vitamins A, D, E, and C on apoptosis and DNA damage in sodium fluoride-treated renal and osteoblast cell lines. Fluoride 2017; 50(3):300-313.
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Jun signaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. Matrix Biol 2007;26 (8): 633–641.
  • Zhang M, Wang A, Xia T, He P. Effects of fluoride on DNA damage, S phase cell-cycle arrest and the expression of NF-kB in primary cultured rat hippocampal neurons. Toxicol Lett 2008;179(1):1–5.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Hekimlik
Bölüm 2020 Volume 4 Number 2
Yazarlar

Sedat ÇETİN (Sorumlu Yazar)
VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, TEMEL BİLİMLER BÖLÜMÜ, VETERİNERLİK BİYOKİMYASI ANABİLİM DALI
0000-0002-6102-8571
Türkiye


Veysel YÜKSEK
VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, ÖZALP MESLEK YÜKSEKOKULU
0000-0001-7432-4989
Türkiye


Semiha DEDE
VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, TEMEL BİLİMLER BÖLÜMÜ, VETERİNERLİK BİYOKİMYASI ANABİLİM DALI
0000-0001-5744-6327
Türkiye


Ayşe USTA
VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, FEN FAKÜLTESİ, KİMYA BÖLÜMÜ
0000-0002-5522-3469
Türkiye


Mehmet TAŞPINAR
AKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI
0000-0002-7598-2665
Türkiye

Yayımlanma Tarihi 30 Ekim 2020
Başvuru Tarihi 21 Nisan 2020
Kabul Tarihi 27 Mayıs 2020
Yayınlandığı Sayı Yıl 2020, Cilt 4, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { tjvr724717, journal = {Turkish Journal of Veterinary Research}, issn = {}, eissn = {2602-3695}, address = {}, publisher = {Ebubekir CEYLAN}, year = {2020}, volume = {4}, pages = {45 - 49}, doi = {}, title = {Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride}, key = {cite}, author = {Çetin, Sedat and Yüksek, Veysel and Dede, Semiha and Usta, Ayşe and Taşpınar, Mehmet} }
APA Çetin, S. , Yüksek, V. , Dede, S. , Usta, A. & Taşpınar, M. (2020). Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride . Turkish Journal of Veterinary Research , 4 (2) , 45-49 . Retrieved from https://dergipark.org.tr/tr/pub/tjvr/issue/57729/724717
MLA Çetin, S. , Yüksek, V. , Dede, S. , Usta, A. , Taşpınar, M. "Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride" . Turkish Journal of Veterinary Research 4 (2020 ): 45-49 <https://dergipark.org.tr/tr/pub/tjvr/issue/57729/724717>
Chicago Çetin, S. , Yüksek, V. , Dede, S. , Usta, A. , Taşpınar, M. "Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride". Turkish Journal of Veterinary Research 4 (2020 ): 45-49
RIS TY - JOUR T1 - Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride AU - Sedat Çetin , Veysel Yüksek , Semiha Dede , Ayşe Usta , Mehmet Taşpınar Y1 - 2020 PY - 2020 N1 - DO - T2 - Turkish Journal of Veterinary Research JF - Journal JO - JOR SP - 45 EP - 49 VL - 4 IS - 2 SN - -2602-3695 M3 - UR - Y2 - 2020 ER -
EndNote %0 Turkish Journal of Veterinary Research Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride %A Sedat Çetin , Veysel Yüksek , Semiha Dede , Ayşe Usta , Mehmet Taşpınar %T Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride %D 2020 %J Turkish Journal of Veterinary Research %P -2602-3695 %V 4 %N 2 %R %U
ISNAD Çetin, Sedat , Yüksek, Veysel , Dede, Semiha , Usta, Ayşe , Taşpınar, Mehmet . "Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride". Turkish Journal of Veterinary Research 4 / 2 (Ekim 2020): 45-49 .
AMA Çetin S. , Yüksek V. , Dede S. , Usta A. , Taşpınar M. Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. TJVR. 2020; 4(2): 45-49.
Vancouver Çetin S. , Yüksek V. , Dede S. , Usta A. , Taşpınar M. Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. Turkish Journal of Veterinary Research. 2020; 4(2): 45-49.
IEEE S. Çetin , V. Yüksek , S. Dede , A. Usta ve M. Taşpınar , "Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride", Turkish Journal of Veterinary Research, c. 4, sayı. 2, ss. 45-49, Eki. 2020