Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Yıl 2020, Cilt 4, Sayı 2, 45 - 49, 30.10.2020

Sedat ÇETİN Veysel YÜKSEK Semiha DEDE Ayşe USTA Mehmet TAŞPINAR

Öz

Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell. Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method. Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while it increased 7 times in the group treated with NaF+lycopene. A significant difference was detected between the groups in terms of gene expression pattern. While the Ripk 3 gene increased slightly in the 24th hour applied lycopene group, it was observed that only lycopene applied group increased 8 times and NaF+lycopene applied group increased in the 9 times Conclusion: Based on the results obtained from this study, it was seen that activation of necrotic genes is important in explaining the molecular basis of cell death from NaF, which is applied as fluorine source, in revealing the molecular basis of the necrotic pathway. It was found that the decrease in cell viability due to NaF increased with lycopene, but the use of lycopene with fluorine also increased necrotic gene expression. Keywords: NaF, in vitro, Lycopene, Necrotic Genes

Anahtar Kelimeler

NaF, in vitro

Öz

Kaynakça

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