The complex nature of the marine environments including a broad array of factors seems inconvenient to have specific indicator organisms alerting the changes in ecological quality based on pollutant inputs to water bodies. Benthic infauna, however, may respond to the pollution-induced spatial qualitychanges in a tidal inlet as they are incapable to avoid from the pollution sources and hence, from low quality of sediment and water. This research suggested that the interaction of sediment metal stressors and the possible associated factors finer grain fractions, estuary position and depth is likely to encounter with a specific distributional pattern of infauna. The communities of macroinfauna in Langstone Harbour were studied spatially from 36 samples collected across four subtidal stations, two each to the upper northern and the southern of the platform. The distinct variations in communities between and within stations and the most contributed species to variations were determined using multivariate analysis techniques. The structured model showed that the measured environmental factors explained 29.4% of infaunal community structure in the harbour with the highest contribution of chromium (6.6%). Environmental patterns suggested the increasing metal deposition in the finergrainedmuddy sediments towards the innermost basin with stagnant and shallower waters.