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Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut

Year 2012, Volume: 2 Issue: 3, 47 - 51, 23.07.2016

Abstract

The aim of this study was to characterize human gut lactic acid bacteria group strains on the basis of their phenotypic profiles. In addition, their in vitro potential probiotic properties were evaluated with a view to identifying potential interesting application. Fifteen strains of lactic acid bacteria were isolated and identified. Among the strains, both biochemical and physiological characteristics differed noticeably and also showed a remarkable heterogeneity. The strains were grouped into the species Lactobacillus gasseri, Lb.casei ssp casei, Lb. delbrueckii ssp lactis, Lb. fermentum and Lb. delbrueckii ssp bulgaricus. These strains have an inhibitory activity against enterobacteria including Escherichia coli and Salmonella sp. A major part of these strains survived at pH 2.5 and in 0.3% bile salts. Additionally they produced no haemolysis, were resistant to kanamycin and adhered to epithelial cells. The results showed that the strains Lb. gasseri HG8 and Lb. fermentum HG3 have the best potential probiotic properties

References

  • Annika, M.M., Manninan, M., Gylienberg, H., 1983. The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. J. Appl. Bacteriol, 55, 241-245.
  • Coconnier, M.H., Bernet, M., Kerne´is, S., Chauvie`re, G., Fourniat, J., Servin, A.L., 1993. Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS. Microbiol. Lett, 110(3), 299-305.
  • Daeschel, M.A., 1989. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food. Technol, 43, 164 -167.
  • Fanaro, S., Chierici, R., Guerrini, P., Vigi, V., 2003. Intestinal microflora in early infancy: composition a development. Acta Paediatr, 441 (Suppl.), 48-55.
  • FAO/WHO., 2002. Guidelines for the evaluation of probiotics in food. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada, 30 April and 1 May, 2002. http://www.who.int/foodsafety/ fs_management/en/probiotic_guidelines.pdf. Accessed 4 July 2009.
  • Garriga, M., Pascual, M., Monfort, J.M., Hugas, M., 1998. Selection of lactobacilli for chicken probiotic adjuncts. J. Appl. Microbiol, 84, 125-132.
  • Gilliland, S.E., Staley, T.E., Bush, L.J., 1984. Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. J. Dairy Sci, 67, 3045 -3051.
  • Idoui, T., Leghouchi, E., Karam, N.E., 2007. Selection of Lactobacillus plantarum BJ0021 for rabbit probiotic adjuncts. I. J. Preb.Pro, 2, 188-193
  • Lin, W.H., Hwang, C.F., Chen, L.W., Tsen, H.Y., 2006. Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food. Microbiol, 23, 74 - 81.
  • Lin, W.H., Yu, B., Jang, S.H., Tsen, H.Y., 2007. Different probiotic properties for Lactobacillus fermentum strain isolated from swine and poultry. Anaerobe, 13, 107-113.
  • Mayur, R.A., Rajiv, K. S., 2002. Selection of Human Isolates of Bifidobacteria for Their Use as robiotics. Ap. Bioch. Biot, 102-103, 81- 98.
  • Mitsuoka, T., 1992. In The Lactic Acid Bacteria, Vol. 1. The Lactic Acid Bacteria in Health and Disease, Wood, B. J. B., ed., Elsevier, London.
  • Saulnier, D.M., Gibson, G.R., Kolida, S., 2008. In vitro effects of selected synbiotics on the human faecal microbiota composition. FEMS. Microbiol. Ecol, 66(3), 516-527.
  • Schillinger, U., Lucke, F.K., 1989. Antibacterial activity of Lactobacillus.sake isolated from meat. Appl. Envir. Microbiol, 55, 1901-1906.
  • Sharpe, M.E., 1979. Identification of lactic acid bacteria, in: Skinner FA, Lovelock DW (Eds), Identification methods for microbiologists, Academic Press, London, UK.
  • Sheehan, V.M., Sleator, R.D., Hill, C., Fitzgerald, G.F., 2007. Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. Microbiology, 153(10), 3563–3571
  • Xanthopoulous, V., Litopoulou-Tzanetaki, E.,Tzanetaki, N., 2000. Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food. Microbiol, 17, 205-215.
Year 2012, Volume: 2 Issue: 3, 47 - 51, 23.07.2016

Abstract

References

  • Annika, M.M., Manninan, M., Gylienberg, H., 1983. The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. J. Appl. Bacteriol, 55, 241-245.
  • Coconnier, M.H., Bernet, M., Kerne´is, S., Chauvie`re, G., Fourniat, J., Servin, A.L., 1993. Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS. Microbiol. Lett, 110(3), 299-305.
  • Daeschel, M.A., 1989. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food. Technol, 43, 164 -167.
  • Fanaro, S., Chierici, R., Guerrini, P., Vigi, V., 2003. Intestinal microflora in early infancy: composition a development. Acta Paediatr, 441 (Suppl.), 48-55.
  • FAO/WHO., 2002. Guidelines for the evaluation of probiotics in food. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada, 30 April and 1 May, 2002. http://www.who.int/foodsafety/ fs_management/en/probiotic_guidelines.pdf. Accessed 4 July 2009.
  • Garriga, M., Pascual, M., Monfort, J.M., Hugas, M., 1998. Selection of lactobacilli for chicken probiotic adjuncts. J. Appl. Microbiol, 84, 125-132.
  • Gilliland, S.E., Staley, T.E., Bush, L.J., 1984. Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. J. Dairy Sci, 67, 3045 -3051.
  • Idoui, T., Leghouchi, E., Karam, N.E., 2007. Selection of Lactobacillus plantarum BJ0021 for rabbit probiotic adjuncts. I. J. Preb.Pro, 2, 188-193
  • Lin, W.H., Hwang, C.F., Chen, L.W., Tsen, H.Y., 2006. Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food. Microbiol, 23, 74 - 81.
  • Lin, W.H., Yu, B., Jang, S.H., Tsen, H.Y., 2007. Different probiotic properties for Lactobacillus fermentum strain isolated from swine and poultry. Anaerobe, 13, 107-113.
  • Mayur, R.A., Rajiv, K. S., 2002. Selection of Human Isolates of Bifidobacteria for Their Use as robiotics. Ap. Bioch. Biot, 102-103, 81- 98.
  • Mitsuoka, T., 1992. In The Lactic Acid Bacteria, Vol. 1. The Lactic Acid Bacteria in Health and Disease, Wood, B. J. B., ed., Elsevier, London.
  • Saulnier, D.M., Gibson, G.R., Kolida, S., 2008. In vitro effects of selected synbiotics on the human faecal microbiota composition. FEMS. Microbiol. Ecol, 66(3), 516-527.
  • Schillinger, U., Lucke, F.K., 1989. Antibacterial activity of Lactobacillus.sake isolated from meat. Appl. Envir. Microbiol, 55, 1901-1906.
  • Sharpe, M.E., 1979. Identification of lactic acid bacteria, in: Skinner FA, Lovelock DW (Eds), Identification methods for microbiologists, Academic Press, London, UK.
  • Sheehan, V.M., Sleator, R.D., Hill, C., Fitzgerald, G.F., 2007. Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. Microbiology, 153(10), 3563–3571
  • Xanthopoulous, V., Litopoulou-Tzanetaki, E.,Tzanetaki, N., 2000. Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food. Microbiol, 17, 205-215.
There are 17 citations in total.

Details

Other ID JA56RM66VD
Journal Section Articles
Authors

Tayeb Idouı This is me

Publication Date July 23, 2016
Published in Issue Year 2012 Volume: 2 Issue: 3

Cite

APA Idouı, T. (2016). Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut. TOJSAT, 2(3), 47-51.
AMA Idouı T. Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut. TOJSAT. July 2016;2(3):47-51.
Chicago Idouı, Tayeb. “Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut”. TOJSAT 2, no. 3 (July 2016): 47-51.
EndNote Idouı T (July 1, 2016) Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut. TOJSAT 2 3 47–51.
IEEE T. Idouı, “Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut”, TOJSAT, vol. 2, no. 3, pp. 47–51, 2016.
ISNAD Idouı, Tayeb. “Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut”. TOJSAT 2/3 (July 2016), 47-51.
JAMA Idouı T. Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut. TOJSAT. 2016;2:47–51.
MLA Idouı, Tayeb. “Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut”. TOJSAT, vol. 2, no. 3, 2016, pp. 47-51.
Vancouver Idouı T. Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut. TOJSAT. 2016;2(3):47-51.