The anti-inflammatory effects of thymoquinone in a rat model of allergic rhinitis

Abstract

Objectives: This study aims to investigate the effect of thymoquinone TQ on airway inflammation in a rat model of allergic rhinitis AR . Materials and Methods: Allergic rhinitis was induced in 42 rats by intraperitoneal sensitization and intranasal challenge with ovalbumin OVA . The animals were divided into six subgroups n=7/per cage : healthy controls, AR group, AR group treated with corticosteroid dexamethasone 1 mg/kg; CS+AR , healthy rats that were given only TQ of 10 mg/kg TQ10 , AR group treated with TQ of 3 mg/kg TQ3+AR and AR group treated with TQ of 10 mg/kg TQ10+AR . We measured the serum levels of interferon-gamma IFN-g , interleukin-4 IL-4 , IL-10, and OVA-specific immunoglobulin E Ig-E . Histopathologic changes in nasal mucosa and expression of tumor necrosis factor-alpha TNF-a and IL-1b were evaluated. Results: Thymoquinone has inhibited the production of the IL-4, OVA-specific IgE, and the expression of TNF-a and IL-1b. It also reduced eosinophil infiltration and edema in the nasal mucosa while it has no increased effect on IFN-g and IL-10. Conclusion: We observed that TQ has multiple suppressive effects on allergic response. Thymoquinone may be considered as a supplemental agent in the treatment of allergic rhinitis.

Keywords

• Interleukin-10, interleukin-1 beta, interleukin-4, ovalbumin-specific IgE, thymoquinone, allergic rhinitis, tumor necrosis factor-alpha

Sıçan alerjik rinit modelinde timokinonun anti-enflamatuvar etkileri

Abstract

Amaç: Bu çalışmada sıçan alerjik rinit AR modelinde timokinonun TQ havayolu enflamasyonu üzerindeki etkisi araştırıldı.Gereç ve Yöntem: Ovalbümin OVA ile intraperitoneal sensitizasyon ve intranazal uygulama ile 42 sıçanda alerjik rinit indüklendi. Hayvanlar altı alt gruba ayrıldı kafes başına n=7 : sağlıklı kontroller, AR grubu, kortikosteroid ile tedavi edilen AR grubu 1 mg/kg deksametazon; CS+AR , sadece 10 mg/kg TQ verilen sağlıklı sıçanlar TQ10 , 3 mg/kg TQ ile tedavi edilen AR grubu TQ3+AR ve 10 mg/kg TQ ile tedavi edilen AR grubu TQ10+AR . İnterferon-gama IFN-g , interlökin-4 IL-4 , IL-10 ve OVA’ya özgü immünoglobülin E’nin Ig-E serum düzeyleri ölçüldü. Nazal mukozadaki histopatolojik değişiklikler ve tümör nekroz faktör-alfa TNF-a ve IL-1b ekspresyonu değerlendirildi.Bulgular: Timokinon, IL-4, OVA’ya özgü IgE ve TNF-a ve IL-1b’nın ekspresyonunu engelledi. Aynı zamanda burun mukozasında eozinofil infiltrasyonunu ve ödemi azalttı, ancak IFN-g ve IL-10 üzerinde artmış bir etki göstermedi.Sonuç: Timokinonun alerjik reaksiyon üzerinde birden fazla baskılayıcı etkiye sahip olduğu gözlemlendi. Timokinon, alerjik rinit tedavisinde yardımcı bir ajan olarak düşünülebilir

Keywords

• İnterlökin-10, interlökin-1 beta, interlökin-4, ovalbümine özgü IgE, timokinon, alerjik rinit, tümör nekroz faktör-alfa

References

1. Bousquet J, Khaltaev N, Cruz AA, Denburg J, Fokkens WJ, Togias A, et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA(2)LEN and AllerGen). Allergy 2008;63:8-160.
2. Kleinjan A, van Nimwegen M, Leman K, Hoogsteden HC, Lambrecht BN. Topical treatment targeting sphingosine-1-phosphate and sphingosine lyase abrogates experimental allergic rhinitis in a murine model. Allergy 2013;68:204-12.
3. El Gazzar M, El Mezayen R, Marecki JC, Nicolls MR, Canastar A, Dreskin SC. Anti-inflammatory effect of thymoquinone in a mouse model of allergic lung inflammation. Int Immunopharmacol 2006;6:1135-42.
4. Kim BY, Shin JH, Park HR, Kim SW, Kim SW. Comparison of antiallergic effects of pneumococcal conjugate vaccine and pneumococcal polysaccharide vaccine in a murine model of allergic rhinitis. Laryngoscope 2013;123:2371-7.
5. Cingi C, Eskiizmir G, Burukoğlu D, Erdoğmuş N, Ural A, Ünlü H. The histopathological effect of thymoquinone on experimentally induced rhinosinusitis in rats. Am J Rhinol Allergy 2011;25:e268-72.
6. Gali-Muhtasib H, Roessner A, Schneider-Stock R. Thymoquinone: a promising anti-cancer drug from natural sources. Int J Biochem Cell Biol 2006;38:1249- 53.
7. Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 2005;5:1749-70.
8. Uz U, Chen B, Palmer JN, Cingi C, Unlu H, Cohen NA. Effects of thymoquinone and montelukast on sinonasal ciliary beat frequency. Am J Rhinol Allergy 2014;28:122-5.

9. Wang W, Zheng M. Nuclear factor kappa B pathway down-regulates aquaporin 5 in the nasal mucosa of rats with allergic rhinitis. Eur Arch Otorhinolaryngol 2011;268:73-81.
10. Xu YY, Liu X, Dai LB, Zhou SH. Effect of Tong Qiao drops on the expression of eotaxin, IL-13 in the nasal mucosa of rats with allergic rhinitis. J Chin Med Assoc 2012;75:524-9.
11. Jung HW, Jung JK, Park YK. Antiallergic effect of Ostericum koreanum root extract on ovalbumin- induced allergic rhinitis mouse model and mast cells. Asian Pac J Allergy Immunol 2011;29:338-48.
12. Settipane RA, Schwindt C. Chapter 15: Allergic rhinitis. Am J Rhinol Allergy 2013;27:52-5.
13. Ying S, Durham SR, Corrigan CJ, Hamid Q, Kay AB. Phenotype of cells expressing mRNA for TH2- type (interleukin 4 and interleukin 5) and TH1-type (interleukin 2 and interferon gamma) cytokines in bronchoalveolar lavage and bronchial biopsies from atopic asthmatic and normal control subjects. Am J Respir Cell Mol Biol 1995;12:477-87.
14. Osguthorpe JD. Pathophysiology of and potential new therapies for allergic rhinitis. Int Forum Allergy Rhinol 2013;3:384-92.
15. Zhang HQ, Sun Y, Xu F. Therapeutic effects of interleukin-1 receptor antagonist on allergic rhinitis of guinea pig. Acta Pharmacol Sin 2003;24:251-5.
16. Jung HW, Jung JK, Park YK. Comparison of the efficacy of KOB03, ketotifen, and montelukast in an experimental mouse model of allergic rhinitis. Int Immunopharmacol 2013;16:254-60.