The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears

Emin Eren; Mustafa Deniz Yılmaz; Yeşim Çekin; Arsenal Sezgin Alikanoğlu; Hülya Eyigör; Gamze Öztürk Yılmaz; Ömer Tarık Selçuk

The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears

Abstract

Objectives: This study aims to investigate the role of Helicobacter pylori H. pylori and gastroesophageal reflux disease GERD in otitis media with effusion OME and to examine the MUC4, MUC5B mucin genes in the guinea pig middle ears to analyze the inflammatory cytokines including tumor necrosis factor-alpha TNF-a , interleukin IL -1beta IL-1b , and IL-8. Patients and Methods: This study was conducted using eight guinea pigs. In the experimental group, H. pylori ATCC strain culture was injected transtympanically with microscopic guidance. The animals in the control group were injected with phosphate buffered saline PBS solution transtympanically. At 12, 24, 48, and 168 h after the injection, transtympanic PBS solution injection and aspiration of the middle ear were applied to all animals. After decapitation, the samples were taken. Results: In the histopathological examination, grading according to the degree of inflammation showed no statistically significant difference between the two groups p>0.05 . No statistically significant difference was determined in the analysis results of MUC4 and MUC5B genes in the middle ears of the two groups p>0.05 , the right and left ear values of IL-8, IL-1b, and TNF-a p=0.999, p=0.610, and p=0.691, respectively . Conclusion: Based on our study results, there was no relationship between H. pylori injection in the middle ear and the histopathological changes of the middle ear mucosa. The results of the analysis of MU

Keywords

Guinea pig, interleukin-1beta, MUC4, MUC5B, otitis media with effusion, tumor necrosis factor-alpha

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Emin Eren This is me

Mustafa Deniz Yılmaz This is me

Yeşim Çekin This is me

Arsenal Sezgin Alikanoğlu This is me

Hülya Eyigör This is me

Gamze Öztürk Yılmaz This is me

Ömer Tarık Selçuk This is me

Publication Date

December 15, 2020

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2020 Volume: 30 Number: 4

IZ

https://izlik.org/JA34LA73BP

Cite

RIS / Bibtex

APA

Eren, E., Yılmaz, M. D., Çekin, Y., Alikanoğlu, A. S., Eyigör, H., Öztürk Yılmaz, G., & Selçuk, Ö. T. (2020). The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. The Turkish Journal of Ear Nose and Throat, 30(4), 131-139. https://izlik.org/JA34LA73BP

AMA

1.Eren E, Yılmaz MD, Çekin Y, et al. The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. Tr-ENT. 2020;30(4):131-139. https://izlik.org/JA34LA73BP

Chicago

Eren, Emin, Mustafa Deniz Yılmaz, Yeşim Çekin, et al. 2020. “The Role of Helicobacter Pylori and Gastroesophageal Reflux Disease in Otitis Media With Effusion and Evaluation of MUC4, MUC5B Mucin Genes and Inflammatory Cytokines in Guinea Pig Middle Ears”. The Turkish Journal of Ear Nose and Throat 30 (4): 131-39. https://izlik.org/JA34LA73BP.

EndNote

Eren E, Yılmaz MD, Çekin Y, Alikanoğlu AS, Eyigör H, Öztürk Yılmaz G, Selçuk ÖT (December 1, 2020) The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. The Turkish Journal of Ear Nose and Throat 30 4 131–139.

IEEE

[1]E. Eren et al., “The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears”, Tr-ENT, vol. 30, no. 4, pp. 131–139, Dec. 2020, [Online]. Available: https://izlik.org/JA34LA73BP

ISNAD

Eren, Emin - Yılmaz, Mustafa Deniz - Çekin, Yeşim - Alikanoğlu, Arsenal Sezgin - Eyigör, Hülya - Öztürk Yılmaz, Gamze - Selçuk, Ömer Tarık. “The Role of Helicobacter Pylori and Gastroesophageal Reflux Disease in Otitis Media With Effusion and Evaluation of MUC4, MUC5B Mucin Genes and Inflammatory Cytokines in Guinea Pig Middle Ears”. The Turkish Journal of Ear Nose and Throat 30/4 (December 1, 2020): 131-139. https://izlik.org/JA34LA73BP.

JAMA

1.Eren E, Yılmaz MD, Çekin Y, Alikanoğlu AS, Eyigör H, Öztürk Yılmaz G, Selçuk ÖT. The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. Tr-ENT. 2020;30:131–139.

MLA

Eren, Emin, et al. “The Role of Helicobacter Pylori and Gastroesophageal Reflux Disease in Otitis Media With Effusion and Evaluation of MUC4, MUC5B Mucin Genes and Inflammatory Cytokines in Guinea Pig Middle Ears”. The Turkish Journal of Ear Nose and Throat, vol. 30, no. 4, Dec. 2020, pp. 131-9, https://izlik.org/JA34LA73BP.

Vancouver

1.Emin Eren, Mustafa Deniz Yılmaz, Yeşim Çekin, Arsenal Sezgin Alikanoğlu, Hülya Eyigör, Gamze Öztürk Yılmaz, Ömer Tarık Selçuk. The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. Tr-ENT [Internet]. 2020 Dec. 1;30(4):131-9. Available from: https://izlik.org/JA34LA73BP