TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc.

Zeynep Danışman; Şule İnan; Esma Özsoy; Emre Yörük; Tapani Yli-mattila

doi:10.23902/trkjnat.1000196

Research Article

TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc.

Year 2022, , 71 - 79, 15.04.2022

Zeynep Danışman , Şule İnan , Esma Özsoy , Emre Yörük , Tapani Yli-mattila

https://doi.org/10.23902/trkjnat.1000196

Abstract

Fusarium avenaceum (Fr.) Sacc. is an important phytopathogen. Fight against F. avenaceum includes primarily fungicide usage. However, novel strategies are needed in a struggle with F. avenaceum. In this study, terpinolene was used against F. avenaceum as an antimicrobial agent, and the harmlessness of terpinolene was tested on two contrast barley genotypes, Hordeum vulgare L. cv. Cervoise and H. vulgare L. cv. Premium. Firstly, minimum inhibitory concentration (MIC) and half inhibitory concentration (IC50) were detected as 6 and 3 µg µL-1. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was informative about the effects of terpinolene on enniatin and zearalenone biosynthesis in F. avenaceum. Terpinolene leads to the potential decreased enniatin and zearalenone biosynthesis in F. avenaceum. However, no significant differences were recorded for gene expression of aurofusariun biosynthesis. In barley, electroconductivity (EC), catalase (CAT) activity, coupled restriction enzyme digestion-random amplification (CRED-RA), and qRT-PCR assays were tested. No significant ion leakage differences (p>0.05) were detected. Similarly, CAT activity and water loss rate (WLR) values in barley sets were not affected (p>0.05) by terpinolene treatment in majority of experiment sets. Relatively low levels of genomic template instability (75-85%) and epigenetics changes (11-20.6%) were recorded in barley due to terpinolene treatment. WRKY6, WRKY24, and WRKY41 expressions were not significantly changed. The findings showed that terpinolene could be accepted as a potential plant protective agent against phytopathogenic fungi.

Keywords

antifungal agent, Fusarium avenaceum, Hordeum vulgare L., qRT-PCR, terpinolene

Supporting Institution

İstanbul Yeni Yüzyil University

Project Number

2020-02

Thanks

The authors are grateful to Dr. Ayşe Feyza Turan Dülger (İstanbul-Turkey) for providing H. vulgare L. primers.

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Year 2022, , 71 - 79, 15.04.2022

Zeynep Danışman , Şule İnan , Esma Özsoy , Emre Yörük , Tapani Yli-mattila

https://doi.org/10.23902/trkjnat.1000196

Abstract

Fusarium avenaceum (Fr.) Sacc. önemli bir bitki patojenidir. Fusarium avenaceum ile mücadele temelde fungisit kullanımını içerir. Ancak yeni stratejilere ihtiyaç duyulmaktadır. Mevcut çalışmada iki zıt arpa genotipi olan Hordeum vulgare L. cv. Cervoise and H. vulgare L. cv. Premium genotiplerinde terpinolenin zararsız etkileri ve F. avenaceum’a karşı antimikrobiyal etkileri araştırıldı. İlk olarak minimum inhibisyon konsantrasyonları (MİK) ve üremeyi baskılayan yarı konsantrasyon (IC50) değerleri 6 and 3 µg µL-1 olarak belirlendi. Gerçek zamanlı polimeraz zincir reaksiyonu (kPZR) F. avenaceum’da zearalenone ve enniatin üretimi üzerinde terpinolenin etkisinin belirlenmesinde bilgi verici oldu. Terpinolene F. avenaceum’da enniatin ve zearalenone üretiminde potansiyel baskılamaya sebep oldu. Buna karşın aurofusariun biyosentez gen analtımında anlamlı farklılık görülmemiştir. Arpada elektrokonduktivite (EK), katalaz (KAT) aktivitesi, CRED-RA analizi ve kPZR analizleri test edildi. EK değerlerinde bilimsel olarak anlamlı (p>0,05) farklılık gözlemlenmedi. Benzer şekilde deney setlerinin çoğunda katalaz aktivitesi ve su kaybetme oranları (SKO) terpinolene uygulamasından etkilenmedi (p>0,05). Arpada terpinolene uygulamasına bağlı olarak göreceli olarak düşük genomik instabilite (%75-85) ve epigenetik değişimler (%11-20,6) kaydedildi. WRKY6, WRKY24 ve WRKY41 gen anlatımları anlamlı farklılık göstermedi. Bulgular terpinolenin fitopatojenik mantarlara karşı potansiyel bir bitki koruma ajanı olarak kabul edilebileceğini göstermektedir.

Project Number

2020-02

References

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18. Goudjil, M., Ladjel, S., Bencheikh, S.E., Hammoya, F., Bensaci, M.B., Zighmi, S. & Mehani, M. 2016. Bioactivity of Artemisia herba alba essential oil against plant pathogenic fungi. Der Pharma Chemica, 8: 46-52.
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20. Gürel, F., Öztürk, N.Z., Yörük, E., Uçarlı, C. & Poyraz, N. 2016. Comparison of expression patterns of selected drought-responsive genes in barley (Hordeum vulgare L.) under shock-dehydration and slow drought treatments. Plant Growth Regulation, 80: 183-193.
21. Harris, E.L.V. & Angal, S. 1989. Protein purification methods, IRL Press at Oxford University Press, Oxford, 336 pp.
22. Irzykowska, L., Bocianowski, J. & Baturo-Cieśniewska, A. 2013. Association of mating-type with mycelium growth rate and genetic variability of Fusarium culmorum. Central European Journal of Biology, 8: 701-711.
23. Hietaniemi, V., Ramo, S., Yli-Mattila, T., Jestoi, M., Peltonen, S., Kartio, M., Sievilainen, E., Koivisto, T. & Parikka, P. 2016. Updated survey of Fusarium species and toxins in finnish cereal grains. Food Additives & Contaminants: Part A, 33: 831-848.
24. Ivic, D., Sever, Z. & Kuzmanovska, B. 2015. In vitro sensitivity of fusarium graminearum, F. avenaceum and F. verticillioides to carbendazim, tebuconazole, flutriafol, metconazole and prochloraz. Pesticides and Phytomedicine, 26: 35-42.
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Details

Primary Language	English
Subjects	Structural Biology, Genetics, Zootechny (Other)
Journal Section	Research Article/Araştırma Makalesi
Authors	Zeynep Danışman 0000-0002-4194-3924 Şule İnan 0000-0001-7921-0913 Esma Özsoy 0000-0002-3727-8466 Emre Yörük 0000-0003-2770-0157 Tapani Yli-mattila 0000-0002-0336-880X
Project Number	2020-02
Publication Date	April 15, 2022
Submission Date	September 24, 2021
Acceptance Date	February 25, 2022
Published in Issue	Year 2022

Cite

APA	Danışman, Z., İnan, Ş., Özsoy, E., Yörük, E., et al. (2022). TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc. Trakya University Journal of Natural Sciences, 23(1), 71-79. https://doi.org/10.23902/trkjnat.1000196
AMA	Danışman Z, İnan Ş, Özsoy E, Yörük E, Yli-mattila T. TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc. Trakya Univ J Nat Sci. April 2022;23(1):71-79. doi:10.23902/trkjnat.1000196
Chicago	Danışman, Zeynep, Şule İnan, Esma Özsoy, Emre Yörük, and Tapani Yli-mattila. “) Sacc”. Trakya University Journal of Natural Sciences 23, no. 1 (April 2022): 71-79. https://doi.org/10.23902/trkjnat.1000196.
EndNote	Danışman Z, İnan Ş, Özsoy E, Yörük E, Yli-mattila T (April 1, 2022) TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc. Trakya University Journal of Natural Sciences 23 1 71–79.
IEEE	Z. Danışman, Ş. İnan, E. Özsoy, E. Yörük, and T. Yli-mattila, “) Sacc”., Trakya Univ J Nat Sci, vol. 23, no. 1, pp. 71–79, 2022, doi: 10.23902/trkjnat.1000196.
ISNAD	Danışman, Zeynep et al. “) Sacc”. Trakya University Journal of Natural Sciences 23/1 (April 2022), 71-79. https://doi.org/10.23902/trkjnat.1000196.
JAMA	Danışman Z, İnan Ş, Özsoy E, Yörük E, Yli-mattila T. TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc. Trakya Univ J Nat Sci. 2022;23:71–79.
MLA	Danışman, Zeynep et al. “) Sacc”. Trakya University Journal of Natural Sciences, vol. 23, no. 1, 2022, pp. 71-79, doi:10.23902/trkjnat.1000196.
Vancouver	Danışman Z, İnan Ş, Özsoy E, Yörük E, Yli-mattila T. TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc. Trakya Univ J Nat Sci. 2022;23(1):71-9.

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