ISOLATION AND IDENTIFICATION OF BIOFILM-FORMING BACTERIA IN FOODS SUPPLIED FOR CONSUMPTION IN OPEN-AIR MARKET STALLS: A CASE STUDY IN AYDIN PROVINCE

Year 2023, , 77 - 84, 15.10.2023

Esin Poyrazoğlu , Mediha Onur

https://doi.org/10.23902/trkjnat.1288588

Abstract

In this study, the isolation and identification of bacteria that form biofilms on various food products sold on open-air market stalls in Aydın Province were aimed. Bacteria were isolated from food surfaces in aseptic conditions and isolated after incubation in the Tryptic Soy Agar medium at 37°C for 24-48 hours. DNA isolations of the isolated bacteria were performed and the PCR products obtained were used for sequencing. The Congo Red Agar method was used to qualitatively analyze biofilm formation. According to this method, bacteria forming black colonies were evaluated as biofilm-positive and were subjected to quantitative analysis using the Microplate method. A total of 67 bacteria were isolated from the sampled food items, of which 7 were strong and 2 were moderate biofilm producers, showing that more importance should be given to food hygiene.

Keywords

Food, Bacteria, Biofilm, Microplate method

Project Number

FEF- 19003

References

• 1. Abdalla, M.A., Siham, E.S., Alian, Y.Y.H.A. & Amel, O.B. 2008. A study of the microbial content of the domestic refrigerators in Khartoum area (Khartoum North.). The Sudan Journal of Veterinary Science and Animal Husbandry, 47: 15-23.
• 2. Altun, H.U. & Sener, B. 2008. Biofilm infections and antibiotic resistance. Hacettepe Medical Journal, 39: 82-88.
• 3. Araujo, P., Lemos, M., Mergulhão, F., Melo, L. & Simões, M. 2011. Antimicrobial resistance to disinfectants in biofilms. pp. 826-834. In: Mendez-Vilas, A. (ed.). Science against microbial pathogens: communicating current research and technological advances. Formatex, Badajoz, 735 pp.
• 4. Ayhan, D. 2016. Biofilm forming microorganisms and characterization of biofilm formation in dairy farms. Hacettepe University Graduate School of Science and Engineering, Department of Food Engineering, Ankara, 299 pp.
• 5. Brenner, D.J., Krieg, N.R, Staley, J.T., & Garrity, G.M. 2004. Bergey’s Manual of Systematic Bacteriology, Edn 2, New York, 72-77 pp.
• 6. Chen, Q., Xie, S., Lou, X., Cheng, S., Liu, X., Zheng, W., Zheng, Z. & Wang, H. 2020. Biofilm formation and prevalence of adhesion genes among Staphylococcus aureus isolates from different food sources. Microbiology Open, 9(1): 1-11.
• 7. Çoban, E.P. & Yaman, F. 2023. Biofilm formation and prevention of bacteria isolated from fish and fish stalls. Acta Aquatica Turcica, 19(1): 21-34.
• 8. Çoban, E.P., Touray, M., Törün, B. & Bıyık, H.H. 2018. Biodiversity of bacteria isolated from home-made wine and vinegar. International Journal of Secondary Metabolite, 5(1): 42-48.
• 9. De Boer, S.H. & Ward, L.J. 1995. PCR detection of Erwinia carotovora subsp. atroceptica associated with potato tissue. Phytopathology, 85(8): 854-858.
• 10. Demir, C. & İnanç, B.B. 2015. Species investigate nasal colonize Staphylococcus species biofilm produced. Journal of Clinical and Analytical Medicine, 6(4): 414-418.
• 11. Douglas, L.J. 2003. Candida biofilms and their role in infection. Trends in Microbiology, 11(1): 30-36.
• 12. Fysun, O., Schmitt, A., Auernhammer, P.T., Rauschnabel, J. & Langowski, H.C. 2019. Electrochemical detection of food-spoiling bacteria using interdigitated platinum microelectrodes. Journal of Microbiological Methods, 161: 63-70.
• 13. Gündoğan, N., Ataol, O. 2012. Biofilm production and determination of DNase activities of Staphylococcus aureus and coagulase negative Staphylococci isolated from meat samples. Turkish Bulletin of Hygiene and Experimental Biology, 69(3): 135-142.
• 14. Gürkan, T., Külahcı, M.B. & Çıtak, S. 2021. Determination of various virulence characteristics, biofilm formation and antibiotic resistance of Enterococcus species isolated from food samples. European Journal of Science and Technology, Special Issue 28: 924-932.
• 15. Hall-Stoodley, L., Costerton, J.W. & Stoodley, P. 2004. Bacterial biofilms: From the natural environment to infectious diseases. Nature Reviews Microbiology, 2: 95-108.
• 16. Jayaraman, A. & Wood, T.K. 2008. Bacterial quorum sensing: Signals, circuits, and implications for biofilms and disease. The Annual Review of Biomedical Engineering, 10: 145-167.
• 17. Keskin, M., Setlek, P. & Demir, S. 2017. Use of color measurement systems in food science and agriculture. International Advanced Researches Engineering Congress Book. Osmaniye Korkut Ata University Publications, Osmaniye, 2350-2359 pp.
• 18. Lo, M., Ngan, W., Tsun, S., Hsing, H., Lau, K., Hung, H., Chan, S., Lai, Y., Yao, Y, Pu, Y. & Habimana, O. 2019. A field study into Hong Kong’s wet markets: Raised questions into the hygienic maintenance of meat contact surfaces and the dissemination of microorganisms associated with nosocomial infections. Frontiers in Microbiology, 10: 1-11.
• 19. Madenci, A.B., Türker, S., Bayramoglu, Z. & Eyiz, V. 2019. Socioeconomic factors affecting consumers' attitudes and perceptions towards food safety: The case of Konya. Helal ve Etik Araştırma Dergisi, 1(1): 48-59.
• 20. Mathur, T., Singhal, S., Khan, S., Upadhyay, D.J., Fatma, T. & Rattan, A. 2006. Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods. Indian Journal of Medical Microbiology, 24(1): 25-29.
• 21. Melo, P.C., Ferreira, L.M., Filho, A.N., Zafalon, L.F., Vicente, H.I.G. & Souza, V. 2013. Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Brazilian Journal of Microbiology, 44(1): 119-124.
• 22. Noronha, F., Silva, S.A., Mena, C., Almeida, G., Hogg, T., Gibbs, P. & Teixeira, P. 2006. Food safety in the domestic environment: Kitchen hygiene. Journal of Food Technology, 4(4): 264-267.
• 23. Özkaya, F.D. & Cömert, M. 2008. Causative factors in food poisoning. Turkish Bulletin Hygiene Experimental Biology, 65: 149-158.
• 24. Sekoai, P.T., Feng, S., Zhou, W., Ngan, W.Y., Pu, Y., Yao, Y., Pan, J. & Habimana, O. 2020. Insights into the microbiological safety of wooden cutting boards used for meat processing in Hong Kong’s wet markets: A focus on food-contact surfaces, cross-contamination and the efficacy of traditional hygiene practices. Microorganisms, 8(579): 1-16.
• 25. Stepanovic, S., Vukovic, D., Hola, V., Bonaventura, G., Djukic, S., Ciricovic, I. & Ruzicka, F. 2007. Quantification of biofilm in microtiterplates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. Acta Pathologica, Microbiologica, et Immunologica Scandinavica, 115(8): 891-899.
• 26. Tekin, K. & Çoban, E.P. 2021. Bacterial diversity in soil samples collected from geothermal fields in Aydın province. pp. 242-250. Proceedings of the 1st International Congress on Natural Sciences (ICNAS-2021) Book. Ataturk University Publications, Erzurum.
• 27. Thomas, J.G. & Nakaishi, L.A. 2006. Managing the complexity of a dynamic biofilm. The Journal of the American Dental Association, 137(3): 10-15.
• 28. Urazel, B., Celikel, A., Karbeyaz, K. & Akkaya, H. 2014. Evaluation of forensic cases for which a report was prepared due to food poisoning. Dicle Medical Journal, 41(1): 113-117.
• 29. Uyanık, T., Bölükbaş, A., Gücükoğlu, A. & Çadırcı, O. 2022. Investigation of biofilm forming ability of some pathogenic bacteria isolated from various food samples and slaughterhouses. Journal of Advances in VetBio Science and Techniques, 7(3): 338-345.
• 30. Yanıkan, E.O. 2020. Investigation of biofilm forming capabilities and antibiofilm susceptibility of meatborne bacteria. Ankara University Institute of Science and Technology, Ankara, 127 pp.
• 31. Zhao, X., Zhao, F., Wang, J. & Zhong, N. 2017. Biofilm formation and control strategies of foodborne pathogens: food safety perspectives. The Royal Society of Chemistry, 7: 36670-36683.