Prunella vulgaris L. VE Prunella grandiflora L.’DEN SAFLAŞTIRILAN ROSMARİNİK ASİTİN FARKLI TÜMÖR HÜCRELERİ ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİ

Year 2017, , 9 - 13, 27.03.2017

Saliha Şahin , Gülçin Tezcan Cevdet Demir Berrin Tunca Gülşah Çeçener , Ünal Egeli

https://doi.org/10.23902/trkjnat.266524

Cited By: 1

Abstract

Bu çalışmada rosmarinik asit bileşiği, Prunella
grandiflora L. ve P. vulgaris L.
türlerinden elde edilen etanol ekstraktlarından saflaştırılmıştır. Saflaştırma
işlemi sırasında elde edilen metanol fraksiyonlarının toplam fenol içeriği
Folin-Ciocalteu yöntemi ile belirlenmiştir. Prunella
L. türlerinden saflaştırılan rosmarinik asitin farklı kanser hücreleri üzerinde
WST-1 (Roche Applied Sciences, Mannheim, Almanya) yöntemiyle sitotoksik doz
çalışmaları yapılmıştır. Buna göre pankreas (PANC-1), prostat (PC-3), kolon
(HT-29) ve meme (MDA-MB 436) kanserleri ile GBM (T98G) hücre hatları ve lenf
dokularında 10-60ng arasında sitotoksik doz belirlenmiştir. Sitotoksik doz
belirleme çalışmaları için 24 ve 48 saat inkübasyon süreleri çalışılmıştır. 48
saat inkübasyon süresi sonunda PC3 hücre hattı için 50ng ve PANC-1, HT-29,
MDA-MB 436 ve T98G hücre hatları için 60ng rosmarinik asit uygulamasında
antiproliferatif etki gözlenmiştir. Sağlıklı hücrelerde rosmarinik asitin
sitotoksik etkisi gözlenmemiştir.

Keywords

Prunella L., izolasyon, rosmarinic asit, antiproliferatif, sitotoksik aktivite

CYTOTOXIC ACTIVITY OF ROSMARINIC ACID ISOLATED FROM Prunella vulgaris L. AND Prunella grandiflora L. IN DIFFERENT TUMOR CELLS

Abstract

Rosmarinic acid was isolated from ethanol
extractions obtained from Prunella
grandiflora L. and P. vulgaris L.
The total phenolic content of ethanol fractions during isolation was determined
by the Folin-Ciocateu method. The cytotoxic doses of the isolated rosmarinic
acid were determined by WST-1 (Roche Applied Sciences, Mannheim, Germany) cell
proliferation assay. A 10-60ng cytotoxic dose was determined for pancreas
(PANC-1), prostate (PC-3), colon (HT-29) and breast (MDA-MB 436) cancers and
GBM (T98G) cell lines and lymphatic tissues. 24 and 48h incubation periods were
applied during dose determinations. An antiproliferative effect was observed at
the end of 48h incubation period with 50ng rosmaniric acid treatment in PC3
cell line and with 60ng treatment in PANC-1, HT-29, MDA-MB 436 and T98G cell
lines. No cytotoxic activity of rosmarinic acid was observed in non-tumor cells

Keywords

Prunella L., isolation, rosmarinic acid, antiproliferative, cytotoxic activity

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