Abstract

Dünyada gerçekleştirilen çalışmalar sonucunda kabakgil familyasındaki bitkileri enfekte eden 50'den fazla virüs varlığı tanımlanmıştır. Virüs tespit metotlarındaki gelişmelere bağlı olarak bu sayıya her geçen gün yenileri eklenmektedir. Son yıllarda tanılanan bu virüs hastalıklarından bir tanesi de Cucumis melo endornavirus (CmEV)'dır. CmEV'nin enfeksiyonu dünyanın farklı ülkelerinden bildirilmiştir. Ancak, ülkemizde şimdiye kadar CmEV varlığının belirlenmesine yönelik bir çalışma gerçekleştirilmemiştir. Bu amaçla, Manisa ve İzmir illerinden virüs ve virüs-benzeri belirti gösteren 59 kabakgil bitkisinden örnekler alınmıştır. Toplanan örnekler ters transkriptaz-polimeraz zincir reaksiyonu ile test edilmiştir. Gerçekleştirilen bu testler sonucunda 47 örnekte CmEV enfeksiyonu tespit edilmiştir. Enfekteli 47 örneğin 44 tanesi kavun (Cucumis melo Linn.), kalan 3 örnek ise acurdur (C. anguria Linn.). Enfekteli örnekler arasından, beş tanesi (üç tanesi kavun, iki tanesi acur) sekans analizleri için seçilmiştir. Gerçekleştirilen sekans analizleri sonucunda CmEV izolatları kendi aralarında nükleotit düzeyinde %93-99, amino asit düzeyinde ise %94-99 benzerlikler gösterdiği belirlenmiştir. Dünya izolatları ile yapılan benzerlik analizleri sonucunda ise nükleotit düzeyinde %93-98, amino asit düzeyinde ise %91-98 benzerlikler tespit edilmiştir. Gerçekleştirilen bu çalışma ile ülkemizde ilk kez CmEV enfeksiyonu kavun ve dünya için yeni bir konukçu kaydı olan acur bitkisinde tespit edilmiştir.

Project Number

FBA-2019-2873

FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY

Abstract

More than 50 viruses causing infection in members of the family Cucurbitaceae have been identified in the world so far. Because of the development of virus detection methods, new viruses are added to the known infectious cases list every day. One of the viruses recently identified is the Cucumis melo endornavirus (CmEV) which has been reported from different countries all over the world. However, no study for determination of CmEV has been done so far in Turkey. For the purpose of this study, 59 cucurbit plants showing virus and virus-like symptoms were collected from Manisa and İzmir provinces in Turkey. The samples were tested, for presence of CmEV, by reverse-transcriptase polymerase chain reaction and CmEV infections were detected in 47 samples, of which 44 were melon (Cucumis melo Linn.), and the remaining were gherkins (C. anguria Linn.). Among the infected samples, five samples (three were melon, and two were gherkin) were chosen for sequence analysis. After evaluating the sequence analysis results, it was shown that the Turkish isolates presented 93%-99% and 93%-98% identities at the nucleotide level and 94%-99% and 91%-98% identities at the amino acid level among each other and worldwide isolates, respectively. To the best of our knowledge, this is the first report of CmEV in gherkin as a new host both in Turkey and worldwide.

Keywords

• Melon,gherkin,virus,CMeV

Supporting Institution

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Project Number

FBA-2019-2873

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