Öz

Bu makale, yeşil çay (Camellia sinensis (L.) Kuntze) ekstraktsiyonu kullanılarak oksitlenmemiş metalik bakır nanopartiküllerinin (CuNPs) mikrodalga destekli yeşil sentezinin hızlı bir protokolünü sunmaktadır. Başarılı biyosentezi tamamladıkten sonra, bakır nanopartiküllerin varlığını doğrulamak ve morfolojilerini ortaya çıkarmak için UV–vis absorpsiyon spektroskopi, Fourier Dönüşümlü Kızıl Ötesi Spektrometresi (FTIR), Enerji dağıtıcı X-ışını analizi (EDX) ile ilişkili Taramalı elektron mikroskobu (SEM), X-ışını Kırınımı (XRD) ve Zeta analiz gibi karakterizasyon teknikleri uygulanılmıştır. Üretilmiş CuNP’lerin UV-vis spektrumu, 570 nm’de karakteristik maksimum absorbansını göstermiştir. Sentezlenen CuNP’lerin, ortalama 45,30 nm büyüklüğünde yuvarlaktan küre şeklinde, birkaç ay boyunca herhangi bir agregasyon olmadan mükemmel stabilite sergilediği bulunmuştur. EDX grafiği, karbon ve oksijenın sırasıyla %17,17 ve %4,87’lik oranlarla birlikte en yüksek miktarda bakır atomunu (%77,96) doğrulanmıştır. Son olarak, sağlıklı fare fibroblast hücreleri (L929 hücre çizgisi) üzerindeki biyosentezlenmiş bu CuNP’lerin non-toksik özelliği doğrulanmıştır ve bu durum, bunların biyolojik araştırmaların yanı sıra geniş kapsamlı uygulamalarda potansiyellerini de göstermektedir.

MICROWAVE IRRADIATION SYSTEM FOR A RAPID SYNTHESIS OF NON-TOXIC METALLIC COPPER NANOPARTICLES FROM GREEN TEA

Abstract

This paper presents a rapid protocol of microwave-assisted green synthesis of non-oxidized metallic copper nanoparticles (CuNPs) using green tea (Camellia sinensis (L.) Kuntze) extract. Following the successful biosynthesis, characterization techniques such as UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) associated with Energy Dispersive X-ray analysis (EDX), X-ray Diffraction (XRD) and Zeta analysis were employed to confirm the presence of metallic CuNPs and reveal their morphology. UV–vis spectrum of fabricated CuNPs indicated its characteristic maximum absorbance at 570 nm. Synthesized CuNPs were found to be round to globular in shape, with average size of 45.30 nm, and showed excellent stability without any aggregation for several months. EDX graph confirmed the highest amount of copper atoms (77.96%) along with carbon and oxygen with the percentage of 17.17% and 4.87%, respectively. The non-toxic nature of the phytosynthesized CuNPs was further established by using healthy mouse fibroblast L929 cell line, which showed their potentiality for biological research and many other applications.

Keywords

• Green tea
• Microwave-assisted green synthesis
• Bioreduction
• Non-toxic metallic CuNPs

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