Abstract

Tip II diyabette kullanılan ilaçların olası yan etkileri, uzun yıllardır kullanılan bitkisel kaynaklara olan eğilimi arttırmaktadır. Senecio vernalis Waldst. & Kit, Türkiye'de yaygın olarak bulunan, gıda ve halk ilacı olarak kullanılan tek yıllık Senecio L. türlerinden biridir. Bu nedenle, bu çalışmada, S. vernalis çiçeklerinin biyoaktif özellikleri (Toplam fenolik madde miktarı (TPC) ve antioksidan kapasite) ve α-amilaz, α-glukozidaz ve lipaz inhibitör aktivite potansiyeli üzerinde optimizasyon ekstraksiyon koşulları araştırıldı. Optimum ekstraksiyon koşulları %69.72 su konsantrasyonunda, 59℃'de 26.15 dakika olarak belirlenmiş ve TPC ve 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) süpürme aktivitesinin en yüksek deneysel değerleri sırasıyla 28,14 mg gallik asit eşdeğeri (GAE) g-1 ve 3165.99 mg troloks eşdeğeri (TE)/100 g numune olarak belirlenmiştir. Tip II diyabette anahtar enzim olan α-amilaz, α-glukozidaz için 100 mg mL-1 konsantrasyonunda sırasıyla %21.32 ve %64.16 inhibisyon gözlemlendi. Senecio vernaris ekstraktı, saptanabilir bir lipaz inhibisyonu göstermedi. Sonuçlar, yüksek bir antioksidan kapasiteye sahip olan S. vernalis'in de önemli bir anti-diyabetik etkiye sahip olduğunu göstermiştir. Senecio vernalis'in gıda ve ilaç gibi birçok endüstride doğal bir kaynak olarak değerlendirilebileceği sonucuna varılabilir. 

EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES

Abstract

The possible side effects of drugs used in type II diabetes are increasing the tendency to herbal resources that have been used for many years. Senecio vernalis Waldst. & Kit is one of the annual Senecio L. species widely distributed in Turkey and used as a food and folk medicine. In this study, optimization of extraction conditions on the bioactive properties (Total phenolic content (TPC) and antioxidant capacity) of the flowers of S. vernalis and the potential of the plant for α-amylase, α-glucosidase, and lipase inhibitory activity were investigated. The optimum extraction conditions were determined at 69.72% water concentration, 59℃ for 26.15 min, and the highest experimental values of TPC and 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) scavenging activity were observed as 28.14 mg gallic acid equivalent (GAE) g-1 and 3165.99 mg trolox equivalent (TE)/100 g sample, respectively. Significant inhibition was observed for α-amylase and α-glucosidase which are the key enzymes in type II diabetes, at a concentration of 100 mg mL-1, with 21.32% and 64.16% respectively. The S. vernalis extracts showed no detectable inhibition of lipase. The results showed that S. vernalis, which has high antioxidant capacity also has a significant anti-diabetic effect. It can be concluded that S. vernalis can be considered a natural resource in many industries such as food and pharmaceuticals. 

Keywords

• Senecio vernalis
• Type II diabetes
• Antioxidant activity
• Extraction optimization

Thanks

The plant material was diagnosed by Prof. Ümit BUDAK, who worked in Yozgat Bozok University, Department of Molecular Biology and Genetics. We would like to thank him for his contribution.

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