Abstract

Bu çalışma, bazı Colchicum L. (Colchicaceae) türlerinin doğal olarak yetiştiği toprağın özelliklerini ve mikrobiyotasını belirlemeyi amaçlamaktadır. Bu amaç ile Colchicum balansae Planch., Colchicum triphyllum Kunze ve Colchicum variegatum L.'nin rizosfer toprak örnekleri analiz edilmiştir. Colchicum balansae toprağının karbon mineralizasyon oranı diğer iki toprağınkinden önemli ölçüde farklıdır (p<0,05). Azot mineralizasyonu açısından, her üç toprak arasında önemli bir fark vardır (p<0,001). Colchicum variegatum rizosferinin en yüksek bakteri çeşitliliğine sahip olduğu belirlendi. 254 bakteri türü üç rizosfer toprağında ortaktı, bakteri türlerinin %35,60'ı C. variegatum toprağına özgüydü, bakteri türlerinin sırasıyla %21,89'u ve %22,67'si C. balansae ve C. triphyllum toprağına özgüydü. Birbirine yakın bölgelerden toplanan C. variegatum ve C. balansae'nin en fazla ortak bakteri türüne sahip olduğu (116), uzak bölgeden gelen C. triphyllum'un ise C. variegatum ile 75 ve C. balansae ile 19'unu paylaştığı tespit edildi. Metagenomik analiz, C. variegatum, C. balansae ve C. triphyllum'un rizosferinde Actinobacteria'nın baskın bakteri filumu olduğunu ortaya koymaktadır. Bunun yanında C. variegatum toprağında Nitrosocosmicus ve halofilik Halobacter'in baskın arke olduğu bulunmuştur. Colchicum triphyllum ve C. balansae topraklarında Saccharomycetales tespit edilirken, Cryptococcus neoformans var. grubii H99 sadece C. balansae toprağında tespit edildi. Colchicum variegatum rizosferinde diğer iki toprak örneğinden organik karbon (%C) ve karbon mineralizasyonu bakımından önemli derecede farklılık (p<0,05) gözlenmiştir. Toprakta azot içeriği bakımından her üç toprakta anlamlı farklılık gözlenmiş olup yarayışlı fosfor içeriklerinde ise C. triphyllum diğerlerinden anlamlı derecede farklıdır (p<0,05). Bu çalışma, rizosfer toprağının biyolojik ve fiziko-kimyasal özelliklerinin, toprak mikrobiyal çeşitliliği ve yoğunluğunun karbon ve azot mineralizasyonuna olan etkilerini göstermektedir. 

Project Number

FYL-2020-12926

COMPARISON OF C AND N MINERALIZATION AND METAGENOME ANALYSIS OF RHIZOSPHERE SOILS BELONGING TO DIFFERENT Colchicum L. SPECIES

Abstract

This study is aimed at determining the characteristics and microbiota of soil upon which some Turkish Colchicum (Colchicaceae) species naturally grows. For this aim the rhizosphere soil samples of Colchicum balansae Planch., Colchicum triphyllum Kunze and Colchicum variegatum L. were analysed in this research. The carbon mineralization rate of C. balansae soil at p<0.05 is significantly different from that of the other two soils. In terms of nitrogen mineralization, significant difference exists between all the three soils (p<0.001). Colchicum variegatum rhizosphere was found to have the highest bacterial diversity. The results revealed that 254 bacterial species were common to the three rhizosphere soils, 35.60% of the bacterial species were unique to C. variegatum soil while 21.89% and 22.67% of the bacterial species were unique to C. balansae and C. triphyllum soil respectively. It was found that C. variegatum and C. balansae collected from areas close to each other had the highest number of common bacterial species, while C. triphyllum from the distant region shared 75 with C. variegatum and 19 with C. balansae. Metagenomics analysis reveals that in the rhizophere of C. variegatum, C. balansae and C. triphyllum, Actinobacteria is dominant at phylum level. Likewise, in C. variegatum soil, Nitrosocosmicus and halophilic Halobacter were found to be the dominant archaea. In the soils of C. triphyllum and C. balansae Saccharomycetales were detected, while Cryptococcus neoformans var. grubii H99 was exclusively detected in C. balansae soil. Significant difference (p<0.05) was observed in C. variegatum rhizosphere soil in terms of organic carbon (C%) and carbon mineralization from the other two soil samples. Significant differences were observed in all three soils in terms of nitrogen content, and the C. triphyllum rhizosphere soil was significantly different from the others in terms of available phosphorus content (p<0.05). This study showed that biological as well as the physico-chemical properties of the rhizosphere soil regulate soil microbial diversity and density and by extension influences their activity which evidently manifests itself in carbon and nitrogen mineralisation.

Keywords

• C. balansae
• C. variegatum
• C. triphyllum
• Soil, carbon and nitrogen mineralization
• Microbiota
• Rhizosphere

Supporting Institution

Çukurova University Research Projects Unit

Project Number

FYL-2020-12926

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