Kritik Tehlikedeki Dianthus ingoldbyi Turrill’in Yavaş Büyüme Koşulları Altında In vitro Korunması

Year 2016, Volume: 17 Issue: 1, 47 - 54, 15.06.2016

Hayati Arda , Sergun Dayan , Çiler Kartal , Necmettin Güler

Abstract

Bu çalışma kritik biçimde tehlike altındaki Balkan endemiği Dianthus ingoldbyi’nin mikroçoğaltımı ve düşük sıcaklık
ve karanlık koşullar altında in vitro sürgünlerin büyümesinin yavaşlatılmasını takiben sürgünlerin anatomik ve morfolojik
karakterlerinin incelenmesi amacıyla yapılmıştır. Bu amaçla, aseptik fidelerden elde edilen aksillar tomurcuklar 1mg/L
Benzilaminopürin (BAP) ve 0,3mg/L Naftalen asetik asit (NAA) içeren Murashige and Skoog (MS) besi yerinde kültüre
alınarak %84,8 oranında rejenerasyon elde edilmiştir. Kültürün 30. gününde eksplant başına sürgün oranı 5,9 iken ortalama
sürgün uzunluğu 3,6cm olmuştur. Bu sürgünler 0,5mg/L NAA içeren MS besi yerine aktarılarak buzdolabında sürekli karanlık
koşullarda ve 4oC sıcaklıkta alt kültüre almaksızın 1-6 ay süresince depolanmıştır. Soğukta depolamanın 6. ayı sonunda
sürgünlerin %58’i hayatta kalmıştır. Deneylerin sonunda hayatta kalan sürgünlerin büyümesinin anlamlı düzeyde yavaşladığı,
anatomik ve morfolojik açıdan herhangi bir deformasyonun bulunmadığı tespit edilmiştir. Soğukta depolamadan normal
koşullara aktarılan sürgünler kontrol grubundan daha iyi gelişmişlerdir. İyice köklenen fidelerin %48’i başarılı biçimde dış
koşullara şaşırtılmıştır. Sonuç olarak bu çalışmada kullanılan soğukta depolama tekniğinin kritik tehlikedeki D. ingoldbyi’nin
in vitro korunmasında etkili bir metot olduğu öne sürülmektedir

Keywords

Soğuk depolama, mikroçoğaltım, anatomi, morfoloji, Balkan endemiği

In vitro CONSERVATION OF CRITICALLY ENDANGERED Dianthus ingoldbyi TURRILL UNDER SLOW GROWTH CONDITIONS

Abstract

This study was performed in order to micropropagate the critically endangered Balkan endemic Dianthus ingoldbyi
and to investigate the anatomical and morphological characteristic of shoots following the slowing down of the growth of in
vitro shoots under cold and dark conditions. For this purpose, axillary buds isolated from aseptic seedlings were cultured on
Murashige and Skoog (MS) medium including 1mg/L Benzylaminopurine (BAP) and 0.3mg/L Naphthaleneacetic acid (NAA)
and a regeneration ratio of 84.8% was obtained. The number of shoots per explant was 5.9 after 30 days from culture initial
and the mean shoot length was 3.6cm. These shoots were transferred to MS medium including 0.5mg/L NAA and stored at 4
oC in a refrigerator in total darkness for 1-6 months without subculturing. 58% of shoots survived after 6 months of cold storage
conditions. Growth of shoots was significantly slowed down and there was no anatomical or morphological deformation at the
end of the experimental period. Shoots transferred to normal conditions from cold storage developed better than the control
group. Well rooted plants were acclimatized to outdoor conditions with a survival rate of 48%. In conclusion, the cold storage
technique used in this study is suggested as an effective method for in vitro conservation of the critically endangered D.
ingoldbyi.

Keywords

Cold storage, micropropagation, anatomy, morphology, Balkan endemic

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