Bu çalışmada, son yıllarda piyasada
satışa sunulan iki adet çok amaçlı kontakt lens dezenfektan solüsyonunun klinik
örneklerden izole edilen Staphylococcus
aureus Rosenbach ve Pseudomonas
aeruginosa Gessard
bakterilerine karşı etkinliği araştırılmıştır. Çok amaçlı kontakt lens
dezenfektan solüsyonlarının antibakteriyal etkinlikleri ISO 14729’da belirtilen
kültüre dayalı mikrobiyolojik yöntem kullanılarak belirlenmiştir. Üretici tarafından
önerilen minimum dezenfeksiyon sürelerine ek olarak belirlenen örnekleme
saatlerindeki bakteri sayıları tespit edilmiş ve ortalama log düşüşleri
hesaplanmıştır. Çalışmada antibakteriyal etkinlikleri test edilen bu kontakt
lens dezenfektan solüsyonlarının 3 farklı lotu kullanılmıştır. Elde edilen sonuçlar, her iki kontakt lens
dezenfektan solüsyonunun üretici tarafından önerilen minimum dezenfeksiyon
süresi sonunda denenen bakterilerin sayılarında en az 3 log düşüş sağladığını
göstermiştir. Kontakt lens solüsyon endüstrisinde gelişen teknolojiler ve
yapılan bilimsel çalışmalar ile yeni formülasyonlar geliştirilmektedir.
Piyasada satışa sunulan bu yeni ürünlerin antibakteriyal aktivitelerinin tam
olarak anlaşılabilmesi için farklı kaynaklardan izole edilen suşların
kullanılacağı ileriki çalışmalara ihtiyaç duyulmaktadır
1. Abjani, F., Khan, N.A., Jung, S.Y. & Siddiqui, R. 2017. Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens. Experimental Parasitology. doi: 10.1016/j.exppara.2017.09.007.
2. Corbin, G.S., Kading, D.L., Powell, S.M., Rosenblatt, B.D., Secor, G.B., Maissa, C.A. & Garofalo, R.J. 2012. Clinical evaluation of a new multi-purpose disinfecting solution in symptomatic wearers of silicone hydrogel contact lenses. Clinical Optometry, 4: 13-24.
3. Devonshire, P., Munro, F.A., Abernethy, C. & Clark, B.J. 1993. Microbial contamination of contact lens cases in the west of Scotland. British Journal of Ophthalmology, 77: 41-45.
4. Gray, T.B., Cursons, R.T.M, Sherwan, J.F. & Rose, P.R. 1995. Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases. British Journal of Ophthalmology, 79: 601-605.
5. International Organization for Standardization ISO/14729. 2001. Ophthalmic optics-Contact lens care products-Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses.
6. Kern, J.R., Kappell, G., Callan, M., McNamee, L., McAnally, C., Walters, R. & Borazjani, R.N. 2011. Antimicrobial properties of a novel contact lens disinfecting solution, OPTI-FREE EverMoist. Contact Lens and Anterior Eye, 34: S30.
7. Kilvington, S. 2000. Through a glass darkly: contact lenses and personal hygiene. Microbiology Today, 27: 66-69.
8. Kuzman, T., Pokupec, R., Kalauz, M., Juri, J., Bujger, Z. & Presecki, A. 2008. A comparative study of antibacterial and antifungal efficacy of soft contact lens disinfecting solutions. Acta Clinica Croatica, 47: 43-48.
9. Lapple, W.J. 2011. The multi-purpose disinfecting solution (MPDS) with unsurpassed disinfection – an easy recommendation for all soft lenses. http://solution.biotrue.com/Portals/64/PDF/Lapple.pdf (Erişim tarihi: Şubat 2013).
10. Larkin, D.F.P., Kilvington, S. & Easty, D.L. 1990. Contamination of contact lens storage cases by Acanthamoeba and bacteria. British Journal of Ophthalmology, 74: 133-135.
11. Manuj, K., Gunderson, C., Troupe, J. & Huber, M.E. 2006. Efficacy of contact lens disinfecting solutions against Staphylococcus aureus and Pseudomonas aeruginosa. Eye and Contact Lens, 32: 216-218.
12. Reindel, W., Cairns, G. & Merchea, M. 2010. Assessment of patient and practitioner satisfaction with Biotrue™ multi-purpose solution for contact lenses. Contact Lens and Anterior Eye, 33S:12-17.
13. Üstüntürk, M. 2014. Kontakt Lens Dezenfektan Solüsyonlarının Antimikrobiyal Etkinliklerinin Araştırılması. İstanbul Üniversitesi Doktora Tezi.
14. Üstüntürk, M. & Zeybek, Z. 2012. Microbial contamination of contact lens storage cases and domestic tap water of contact lens wearers. Wiener Klinische Wochenschrift, 124: 17-22.
15. Üstüntürk M. & Zeybek Z. 2014. Çeşitli kontakt lens dezenfektan solüsyonlarının standart ve çevresel bakteriler üzerine etkileri. 22.Ulusal Biyoloji Kongresi, 23-27 Haziran, Eskişehir, 1502.
16. Yung, M.S., Boost, M., Cho, P. & Yap, M. 2007. Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic and Physiological Optics, 27: 11-21.
17. Zhu, H., Ding, A., Bandara, M., Willcox, M.D.P. & Stapleton, F. 2007. Broad spectrum of antibacterial activity of a new multipurpose disinfectant solution. Eye and Contact Lens, 33: 278-283.
Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens
Year 2017,
Volume: 18 Issue: 2, 163 - 166, 27.11.2017
In this
study, the efficacy of two recently marketed multipurpose contact lens
disinfecting solutions against Staphylococcus
aureus Rosenbach and Pseudomonas
aeruginosa Gessard bacteria
isolated from clinical specimens were investigated. The antibacterial activities of multipurpose contact lens
disinfecting solutions were determined by microbiological culture method
described in ISO 14729 guidelines. Bacterial counts were obtained for sampling
intervals determined in addition to the manufacturer’s minimum recommended
disinfection time and mean log reductions were calculated for each interval.
Three different lots of each contact lens disinfecting solutions were used. The resultsshowed that both contact lens disinfecting solutions provided at
least 3 log reductions for all bacteria tested at the end of the manufacturer’s
minimum recommended disinfection time. New formulations are being developed in contact lens
disinfecting solution industry by new technologies and scientific researches.
Further studies on strains isolated from different sources are needed in order
to a complete understanding of these newly marketed contact lens solutions.
1. Abjani, F., Khan, N.A., Jung, S.Y. & Siddiqui, R. 2017. Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens. Experimental Parasitology. doi: 10.1016/j.exppara.2017.09.007.
2. Corbin, G.S., Kading, D.L., Powell, S.M., Rosenblatt, B.D., Secor, G.B., Maissa, C.A. & Garofalo, R.J. 2012. Clinical evaluation of a new multi-purpose disinfecting solution in symptomatic wearers of silicone hydrogel contact lenses. Clinical Optometry, 4: 13-24.
3. Devonshire, P., Munro, F.A., Abernethy, C. & Clark, B.J. 1993. Microbial contamination of contact lens cases in the west of Scotland. British Journal of Ophthalmology, 77: 41-45.
4. Gray, T.B., Cursons, R.T.M, Sherwan, J.F. & Rose, P.R. 1995. Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases. British Journal of Ophthalmology, 79: 601-605.
5. International Organization for Standardization ISO/14729. 2001. Ophthalmic optics-Contact lens care products-Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses.
6. Kern, J.R., Kappell, G., Callan, M., McNamee, L., McAnally, C., Walters, R. & Borazjani, R.N. 2011. Antimicrobial properties of a novel contact lens disinfecting solution, OPTI-FREE EverMoist. Contact Lens and Anterior Eye, 34: S30.
7. Kilvington, S. 2000. Through a glass darkly: contact lenses and personal hygiene. Microbiology Today, 27: 66-69.
8. Kuzman, T., Pokupec, R., Kalauz, M., Juri, J., Bujger, Z. & Presecki, A. 2008. A comparative study of antibacterial and antifungal efficacy of soft contact lens disinfecting solutions. Acta Clinica Croatica, 47: 43-48.
9. Lapple, W.J. 2011. The multi-purpose disinfecting solution (MPDS) with unsurpassed disinfection – an easy recommendation for all soft lenses. http://solution.biotrue.com/Portals/64/PDF/Lapple.pdf (Erişim tarihi: Şubat 2013).
10. Larkin, D.F.P., Kilvington, S. & Easty, D.L. 1990. Contamination of contact lens storage cases by Acanthamoeba and bacteria. British Journal of Ophthalmology, 74: 133-135.
11. Manuj, K., Gunderson, C., Troupe, J. & Huber, M.E. 2006. Efficacy of contact lens disinfecting solutions against Staphylococcus aureus and Pseudomonas aeruginosa. Eye and Contact Lens, 32: 216-218.
12. Reindel, W., Cairns, G. & Merchea, M. 2010. Assessment of patient and practitioner satisfaction with Biotrue™ multi-purpose solution for contact lenses. Contact Lens and Anterior Eye, 33S:12-17.
13. Üstüntürk, M. 2014. Kontakt Lens Dezenfektan Solüsyonlarının Antimikrobiyal Etkinliklerinin Araştırılması. İstanbul Üniversitesi Doktora Tezi.
14. Üstüntürk, M. & Zeybek, Z. 2012. Microbial contamination of contact lens storage cases and domestic tap water of contact lens wearers. Wiener Klinische Wochenschrift, 124: 17-22.
15. Üstüntürk M. & Zeybek Z. 2014. Çeşitli kontakt lens dezenfektan solüsyonlarının standart ve çevresel bakteriler üzerine etkileri. 22.Ulusal Biyoloji Kongresi, 23-27 Haziran, Eskişehir, 1502.
16. Yung, M.S., Boost, M., Cho, P. & Yap, M. 2007. Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic and Physiological Optics, 27: 11-21.
17. Zhu, H., Ding, A., Bandara, M., Willcox, M.D.P. & Stapleton, F. 2007. Broad spectrum of antibacterial activity of a new multipurpose disinfectant solution. Eye and Contact Lens, 33: 278-283.
Üstüntürk-onan, M. (2017). Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens. Trakya University Journal of Natural Sciences, 18(2), 163-166. https://doi.org/10.23902/trkjnat.319754
AMA
Üstüntürk-onan M. Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens. Trakya Univ J Nat Sci. December 2017;18(2):163-166. doi:10.23902/trkjnat.319754
Chicago
Üstüntürk-onan, Miray. “Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens”. Trakya University Journal of Natural Sciences 18, no. 2 (December 2017): 163-66. https://doi.org/10.23902/trkjnat.319754.
EndNote
Üstüntürk-onan M (December 1, 2017) Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens. Trakya University Journal of Natural Sciences 18 2 163–166.
IEEE
M. Üstüntürk-onan, “Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens”, Trakya Univ J Nat Sci, vol. 18, no. 2, pp. 163–166, 2017, doi: 10.23902/trkjnat.319754.
ISNAD
Üstüntürk-onan, Miray. “Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens”. Trakya University Journal of Natural Sciences 18/2 (December 2017), 163-166. https://doi.org/10.23902/trkjnat.319754.
JAMA
Üstüntürk-onan M. Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens. Trakya Univ J Nat Sci. 2017;18:163–166.
MLA
Üstüntürk-onan, Miray. “Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens”. Trakya University Journal of Natural Sciences, vol. 18, no. 2, 2017, pp. 163-6, doi:10.23902/trkjnat.319754.
Vancouver
Üstüntürk-onan M. Efficacy of Contact Lens Solutions Against Bacteria Isolated from Clinical Specimens. Trakya Univ J Nat Sci. 2017;18(2):163-6.
You can reach the journal's archive between the years of 2000-2011 via https://dergipark.org.tr/en/pub/trakyafbd/archive (Trakya University Journal of Natural Sciences (=Trakya University Journal of Science)