Yıl 2019, Cilt 20 , Sayı 1, Sayfalar 27 - 34 2019-04-15

BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE

Müslüm Altun [1]


Polyglutamic acid (PGA) is water-soluble and biodegradable polymer with high production cost. For feasible PGA production, feather hydrolysate (FH) was used as fermentation substrate. 30L fermentation of native feather was realized to obtain keratinase enzyme using Streptomyces pactum DSM 40530. Fermentation broth was concentrated by cross-flow filtration where the enzyme activity increased by 8.75-fold and 8×103UL-1d-1 of enzyme activity was the optimum for achieving 75% degradation per gram of feather. 40g/L of FH was used with different media compositions to produce PGA using Bacillus licheniformis 9945a. Among four different cultivation where L-glutamate, tri-sodium citrate and glycerol were used as the constituents of Medium E, highest yields of γ-PGA and cell dry matter (CDM) were obtained from cultivation-1, at 5.4±0.4 and 8.6±0.5g/L, respectively, despite the culture media did not contain glutamic acid. In cultivation-2, which was not only missing glutamate but also citrate, the γ-PGA and CDM yielded 3.2±0.2 and 7.8±0.4g/L, respectively whereas it was only 1.9±0.2 and 4.2+0.4g/L when FH was used as the sole substrate in cultivation-3. When cultivation-4 was adopted where only glycerol was missing, the γ-PGA and CDM yields slightly increased to 2.3±0.2 and 5.5±0.3g/L, respectively. This is the first study that achieved the production of γ-PGA from FH.


Poliglutamik asit (PGA) yüksek üretim maliyeti olan, suda çözünen ve biyobozunabilir bir polimerdir. Bu çalışmada, fizıbıl PGA üretimi için tavuk tüyü hidrolizatları (TH) fermantasyon substratı olarak kullanılmıştır. Keratinaz enzimi eldesi için, Streptomyces pactum DSM 40530 ile doğal tavuk tüyleri fermente (30L) edilmiştir. Fermantasyon çözeltisindeki enzim aktivitesi, çapraz akışlı filtrasyon ünitesi kullanılarak 8,75 kat deriştirilmiştir. Enzim aktivitesi (8×103UL-1gün-1), gram tavuk tüyü başına %75’lik bozunma için optimum değer olarak belirlenmiştir. PGA üretimi için Bacillus licheniformis 9945a kullanılmış ve 40g/L TH kullanılarak farklı kompozisyonlarda besiyeri çözeltileri hazırlanmıştır. Besiyeri-E bileşenleri olan L-glutamat, sodyum sitrat ve gliserol kullanılarak dört farklı kompozisyonda besiyeri hazırlanmıştır. Bunlar arasında en fazla PGA ve kuru hücre maddesi (KHM) üretimi, glutamik asit içermeyen bir numaralı kültürde, sırasıyla 5,4±0,4 and 8,6±0,5g/L olarak gerçekleşmiştir. Glutamik asitin yanı sıra sitrik asitin de olmadığı iki numaralı kültürde PGA ve KHM değerleri sırasıyla 3,2±0,2 ve 7,8±0,4g/L olurken, sadece TH’nin substrat olarak kullanıldığı üç numaralı kültürde yalnızca 1,9±0,2 ve 4,2+0,4g/L değerleri elde edilmiştir. Sadece gliserolün kullanılmadığı dört numaralı kültürde ise PGA ve KHM değerleri bir miktar yükselmiş ve sırasıyla 2,3±0,2 ve 5,5±0,3g/L değerlerini almıştır. Yapılan çalışma PGA üretiminin TH kullanılarak yapıldığı İlk çalışma olmuştur.

γ-Poly(glutamic acid), γ-PGA, feather hydrolysate, keratinolytic activity, feather
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Birincil Dil en
Konular Fen, Çevre Bilimleri
Bölüm Araştırma Makalesi/Research Article
Yazarlar

Orcid: 0000-0003-2691-7370
Yazar: Müslüm Altun (Sorumlu Yazar)
Kurum: ADIYAMAN ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 29 Temmuz 2018
Kabul Tarihi : 4 Mart 2019
Yayımlanma Tarihi : 15 Nisan 2019

Bibtex @araştırma makalesi { trkjnat448851, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, eissn = {2528-9691}, address = {}, publisher = {Trakya Üniversitesi}, year = {2019}, volume = {20}, pages = {27 - 34}, doi = {10.23902/trkjnat.448851}, title = {BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE}, key = {cite}, author = {Altun, Müslüm} }
APA Altun, M . (2019). BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE. Trakya University Journal of Natural Sciences , 20 (1) , 27-34 . DOI: 10.23902/trkjnat.448851
MLA Altun, M . "BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE". Trakya University Journal of Natural Sciences 20 (2019 ): 27-34 <https://dergipark.org.tr/tr/pub/trkjnat/issue/44590/448851>
Chicago Altun, M . "BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE". Trakya University Journal of Natural Sciences 20 (2019 ): 27-34
RIS TY - JOUR T1 - BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE AU - Müslüm Altun Y1 - 2019 PY - 2019 N1 - doi: 10.23902/trkjnat.448851 DO - 10.23902/trkjnat.448851 T2 - Trakya University Journal of Natural Sciences JF - Journal JO - JOR SP - 27 EP - 34 VL - 20 IS - 1 SN - 2147-0294-2528-9691 M3 - doi: 10.23902/trkjnat.448851 UR - https://doi.org/10.23902/trkjnat.448851 Y2 - 2019 ER -
EndNote %0 Trakya University Journal of Natural Sciences BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE %A Müslüm Altun %T BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE %D 2019 %J Trakya University Journal of Natural Sciences %P 2147-0294-2528-9691 %V 20 %N 1 %R doi: 10.23902/trkjnat.448851 %U 10.23902/trkjnat.448851
ISNAD Altun, Müslüm . "BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE". Trakya University Journal of Natural Sciences 20 / 1 (Nisan 2019): 27-34 . https://doi.org/10.23902/trkjnat.448851
AMA Altun M . BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE. Trakya Univ J Nat Sci. 2019; 20(1): 27-34.
Vancouver Altun M . BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE. Trakya University Journal of Natural Sciences. 2019; 20(1): 34-27.