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OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS

Yıl 2019, Cilt: 20 Sayı: 2, 129 - 134, 15.10.2019
https://doi.org/10.23902/trkjnat.571255

Öz

Plant mutants are
important bio-resources for crop breeding and functional gene studies. In the
present study, conventional chemical mutagenesis technique was combined with
somatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. For
this purpose, 0-5 mM Sodium Azide (NaN3) was applied for 30 minutes
to embryonic calli under in vitro
conditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99).
Treated and non-treated calli were put in somatic embryo induction media, and 3
and 4 mM NaN3 were determined as optimum mutation doses for somatic
embryo induction.
The obtained somatic embryos from these optimum mutagen doses were then
screened for tolerance in regeneration media containing 125 mM NaCl to be used
to improve tolerance to salt stress. In NaN3 treatment, 14 mutants
with moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination with
chemical mutagenesis may be a useful approach for accelerating breeding
strategies to create enough genetic variation in populations and to get fourth
generation putative salt tolerant wheat mutant lines it less than 1.5 years.

Destekleyen Kurum

The Research Fund of Istanbul University

Proje Numarası

BEK-2017-24939, 24750, 45260 and 49463

Kaynakça

  • 1. Ahmad, I., Nasir, I.A., Saleem, M., Haider, M., Javed, M.A., Javed, M.A., Latif, Z. & Husnain, T. 2010. In vitro induction of mutation in potato cultivars. Pakistan Journal of Phytopathology, 22(1): 51-57.
  • 2. Al-Qurainy, F. & Khan, S. 2009. Mutagenic effects of sodium azide and its application in crop improvement. World Applied Sciences Journal, 6(12): 1589-1601.
  • 3. Bidabadi, S.S., Mahmood, M., Meon, S., Wahab, Z. & Ghobadi, C. 2011. Evaluation of in vitro water stress tolerance among EMS –induced variants of banana (Musa spp., AAA), using morphological, physiological and molecular traits. Journal of Crop Science and Biotechnology, 14: 255-263.
  • 4. El-Sayed, E.H., Mahfouze, S.A., Shaltout, A.D., El-Dougdoug, K.A. & Sayed, R.A. 2012. Chemical mutation induction in vitro cultured shoot tip of banana cv. Grand Nain and for resistance some virus diseases. International Journal of Virology, 8: 178-190.
  • 5. Gamborg, O.L., Miller, R.A. & Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-158.
  • 6. Ganesan, M., Bhanumathi, P. & Jayabalan, N. 2005. Mutagenic effect of sodium azide on somatic embryo regeneration and root growth of cotton (Gossypium hirsutum L. CV. SVPR2). Journal of Agricultural Technology, 365-380.
  • 7. He, J., Hu, Y., Li, W.C. & Fu, F.L. 2009. Drought tolerant mutant induced by gamma-ray and sodium azide from maize calli. Maize Genetics Cooperation Newsletter, 83: 53-55.
  • 8. Hoagland, D.R. & Arnon, D.I. 1950. The Water Culture Method For Growing Plant Without Soil. University of California Berkley Press, CA, 347 pp.
  • 9. Hossain, Z., Mandal, A.K., Datta, S.K. & Biswas, A.K. 2006. Development of NaCl-tolerant strain in Chrysanthemum morifolium Ramat. through in vitro mutagenesis. Plant Biology, 8: 450-461.
  • 10. Hububat Sektör Raporu 2017. http://www.tmo.gov.tr/Upload/Document/hububatsektorraporu2017.pdf (Date accessed: 6.05.2019)
  • 11. ul-Haq, I., Memon, S., Gill, N.P. & Rajput, M.T. 2011. Regeneration of plantlets under NaCl stress from NaN3 treated sugarcane explants. African Journal of Biotechnology, 10: 16152-16156.
  • 12. Jain, M.S. 2010. Mutagenesis in crop improvement under the climate change. Romanian Biotech Letter, 15: 88-106.
  • 13. Kanber, R., Çullu, M.A., Kendirli, B., Antepli, S. & Yılmaz, N. 2005. Sulama, drenaj ve tuzluluk, 213-251. Paper presented at the Türkiye Ziraat Mühendisliği 6. Teknik Kongresi, Cilt I, 3-7 Ocak, Ankara-Turkey.
  • 14. Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • 15. Olsen, O., Wang, X. & Von Wetttesin, D. 1993. Sodium azide mutagenesis: Preferential generation of AT -> GC transitions in the barley Antl8 gene. Proceedings of the National Academy of Sciences of the United States of America, 90: 8043-8047.
  • 16. Ozgen, M., Turet, M., Altinok, S. & Sancak, C. 1998. Effects callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Reporter, 18: 331-335.
  • 17. Serrat, X., Esteban, R., Guibourt, N., Moysset, L., Nogués, S. & Lalanne, E. 2014. EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations. Plant Methods, 10(1): 5.
  • 18. Shrivastava, P. & Kumar, R. 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22: 123-131.
  • 19. Suprasanna, P., Jain, S.M., Ochatt, S.J., Kulkarni, V.M. & Predieri, S. 2012. Application of in vitro techniques in mutation breeding of vegetatively propagated crop. Pp. 371-385. In: Shu, Q.Y., Forster, B.P. & Nakagawa, H. (eds) Plant Mutation Breeding and Biotechnology. Join FAO/IAEA Programme Nuclear Techniques in Food and Agriculture, 595 pp.
  • 20. Wang, W. Vinocur, B. & Altman, A. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218: 1-14.
  • 21. Wannajindaporn, A., Poolsawat, O., Chaowiset, W. & Tantasawat, P.A. 2014. Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants. Genetics and Molecular Research, 13: 5333-5342.
  • 22. Yumurtaci, A. & Uncuoglu, A.A. 2012. Tissue specific responses alter the biomass accumulation in wheat under gradual and sudden salt stress. Journal of Stress Physiology and Biochemistry, 8: 143-156.
  • 23. Zair, I., Chlyah, A., Sabounji, K., Tittahsen, M. & Chlyah, H. 2003. Salt tolerance improvement in some wheat cultivars after application of in vitro selection pressure. Plant Cell Tissue and Organ Culture, 73: 237-244.
  • 24. Zar, J.H. 1984. Biostatistical Analysis. Prentice-Hall Inc., Englewood Cliffs, Jersey, 620 pp.
Yıl 2019, Cilt: 20 Sayı: 2, 129 - 134, 15.10.2019
https://doi.org/10.23902/trkjnat.571255

Öz

Bitki
mutantları, bitki ıslahı ve fonksiyonel gen çalışmaları için önemli biyo-kaynaklardır.
Mevcut çalışmada, tuza toleranslı aday mutant buğday hatları elde etmek için
somatik embriyogenez ile konvansiyonel kimyasal mutajenez tekniği
birleştirildi. Bu amaçla; ekmeklik buğdayda (Triticum aestivum L. cv. Adana 99) genetik varyasyonlar yaratmak
için embriyonik kalluslara 0-5 mM Sodyum Azid (NaN3), 30 dakika
boyunca in vitro ortamda uygulandı.
İşlem görmüş ve görmemiş kalluslar, somatik embriyo teşvik ortamına konularak
somatik embriyo teşviki için 3 ve 4 mM NaN3 uygulaması optimum
mutasyon dozları olarak tespit edildi. Ardından bu mutasyon dozları ile muamele
edilen kalluslardan elde edilen somatik embriyolar tuz stresine tolerans
geliştirmek için kullanılacak 125 mM NaCl içeren rejenerasyon ortamlarında
tolerans açısından tarandı. NaN3 muamelesinde, orta düzeyde tuza
toleransı olan 14 mutant elde edildi. Elde edilen sonuçlar; kimyasal mutagenez
ile kombine halde in vitro teknik
uygulamasının, popülasyonlarda yeterli genetik varyasyon oluşturmak ve 1.5
yıldan daha az bir sürede dördüncü jenerasyon tuz toleranslı aday buğday mutant
hatlarını ıslah sürecini hızlandırarak elde etmek için kullanışlı bir yöntem
olabileceğini göstermektedir.

Proje Numarası

BEK-2017-24939, 24750, 45260 and 49463

Kaynakça

  • 1. Ahmad, I., Nasir, I.A., Saleem, M., Haider, M., Javed, M.A., Javed, M.A., Latif, Z. & Husnain, T. 2010. In vitro induction of mutation in potato cultivars. Pakistan Journal of Phytopathology, 22(1): 51-57.
  • 2. Al-Qurainy, F. & Khan, S. 2009. Mutagenic effects of sodium azide and its application in crop improvement. World Applied Sciences Journal, 6(12): 1589-1601.
  • 3. Bidabadi, S.S., Mahmood, M., Meon, S., Wahab, Z. & Ghobadi, C. 2011. Evaluation of in vitro water stress tolerance among EMS –induced variants of banana (Musa spp., AAA), using morphological, physiological and molecular traits. Journal of Crop Science and Biotechnology, 14: 255-263.
  • 4. El-Sayed, E.H., Mahfouze, S.A., Shaltout, A.D., El-Dougdoug, K.A. & Sayed, R.A. 2012. Chemical mutation induction in vitro cultured shoot tip of banana cv. Grand Nain and for resistance some virus diseases. International Journal of Virology, 8: 178-190.
  • 5. Gamborg, O.L., Miller, R.A. & Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-158.
  • 6. Ganesan, M., Bhanumathi, P. & Jayabalan, N. 2005. Mutagenic effect of sodium azide on somatic embryo regeneration and root growth of cotton (Gossypium hirsutum L. CV. SVPR2). Journal of Agricultural Technology, 365-380.
  • 7. He, J., Hu, Y., Li, W.C. & Fu, F.L. 2009. Drought tolerant mutant induced by gamma-ray and sodium azide from maize calli. Maize Genetics Cooperation Newsletter, 83: 53-55.
  • 8. Hoagland, D.R. & Arnon, D.I. 1950. The Water Culture Method For Growing Plant Without Soil. University of California Berkley Press, CA, 347 pp.
  • 9. Hossain, Z., Mandal, A.K., Datta, S.K. & Biswas, A.K. 2006. Development of NaCl-tolerant strain in Chrysanthemum morifolium Ramat. through in vitro mutagenesis. Plant Biology, 8: 450-461.
  • 10. Hububat Sektör Raporu 2017. http://www.tmo.gov.tr/Upload/Document/hububatsektorraporu2017.pdf (Date accessed: 6.05.2019)
  • 11. ul-Haq, I., Memon, S., Gill, N.P. & Rajput, M.T. 2011. Regeneration of plantlets under NaCl stress from NaN3 treated sugarcane explants. African Journal of Biotechnology, 10: 16152-16156.
  • 12. Jain, M.S. 2010. Mutagenesis in crop improvement under the climate change. Romanian Biotech Letter, 15: 88-106.
  • 13. Kanber, R., Çullu, M.A., Kendirli, B., Antepli, S. & Yılmaz, N. 2005. Sulama, drenaj ve tuzluluk, 213-251. Paper presented at the Türkiye Ziraat Mühendisliği 6. Teknik Kongresi, Cilt I, 3-7 Ocak, Ankara-Turkey.
  • 14. Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • 15. Olsen, O., Wang, X. & Von Wetttesin, D. 1993. Sodium azide mutagenesis: Preferential generation of AT -> GC transitions in the barley Antl8 gene. Proceedings of the National Academy of Sciences of the United States of America, 90: 8043-8047.
  • 16. Ozgen, M., Turet, M., Altinok, S. & Sancak, C. 1998. Effects callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Reporter, 18: 331-335.
  • 17. Serrat, X., Esteban, R., Guibourt, N., Moysset, L., Nogués, S. & Lalanne, E. 2014. EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations. Plant Methods, 10(1): 5.
  • 18. Shrivastava, P. & Kumar, R. 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22: 123-131.
  • 19. Suprasanna, P., Jain, S.M., Ochatt, S.J., Kulkarni, V.M. & Predieri, S. 2012. Application of in vitro techniques in mutation breeding of vegetatively propagated crop. Pp. 371-385. In: Shu, Q.Y., Forster, B.P. & Nakagawa, H. (eds) Plant Mutation Breeding and Biotechnology. Join FAO/IAEA Programme Nuclear Techniques in Food and Agriculture, 595 pp.
  • 20. Wang, W. Vinocur, B. & Altman, A. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218: 1-14.
  • 21. Wannajindaporn, A., Poolsawat, O., Chaowiset, W. & Tantasawat, P.A. 2014. Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants. Genetics and Molecular Research, 13: 5333-5342.
  • 22. Yumurtaci, A. & Uncuoglu, A.A. 2012. Tissue specific responses alter the biomass accumulation in wheat under gradual and sudden salt stress. Journal of Stress Physiology and Biochemistry, 8: 143-156.
  • 23. Zair, I., Chlyah, A., Sabounji, K., Tittahsen, M. & Chlyah, H. 2003. Salt tolerance improvement in some wheat cultivars after application of in vitro selection pressure. Plant Cell Tissue and Organ Culture, 73: 237-244.
  • 24. Zar, J.H. 1984. Biostatistical Analysis. Prentice-Hall Inc., Englewood Cliffs, Jersey, 620 pp.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Araştırma Makalesi/Research Article
Yazarlar

Ayşe Şen 0000-0002-1690-4536

Fatma Sarsu Bu kişi benim 0000-0002-1690-4536

Proje Numarası BEK-2017-24939, 24750, 45260 and 49463
Yayımlanma Tarihi 15 Ekim 2019
Gönderilme Tarihi 28 Mayıs 2019
Kabul Tarihi 7 Ekim 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 20 Sayı: 2

Kaynak Göster

APA Şen, A., & Sarsu, F. (2019). OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS. Trakya University Journal of Natural Sciences, 20(2), 129-134. https://doi.org/10.23902/trkjnat.571255
AMA Şen A, Sarsu F. OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS. Trakya Univ J Nat Sci. Ekim 2019;20(2):129-134. doi:10.23902/trkjnat.571255
Chicago Şen, Ayşe, ve Fatma Sarsu. “OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS”. Trakya University Journal of Natural Sciences 20, sy. 2 (Ekim 2019): 129-34. https://doi.org/10.23902/trkjnat.571255.
EndNote Şen A, Sarsu F (01 Ekim 2019) OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS. Trakya University Journal of Natural Sciences 20 2 129–134.
IEEE A. Şen ve F. Sarsu, “OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS”, Trakya Univ J Nat Sci, c. 20, sy. 2, ss. 129–134, 2019, doi: 10.23902/trkjnat.571255.
ISNAD Şen, Ayşe - Sarsu, Fatma. “OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS”. Trakya University Journal of Natural Sciences 20/2 (Ekim 2019), 129-134. https://doi.org/10.23902/trkjnat.571255.
JAMA Şen A, Sarsu F. OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS. Trakya Univ J Nat Sci. 2019;20:129–134.
MLA Şen, Ayşe ve Fatma Sarsu. “OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS”. Trakya University Journal of Natural Sciences, c. 20, sy. 2, 2019, ss. 129-34, doi:10.23902/trkjnat.571255.
Vancouver Şen A, Sarsu F. OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS. Trakya Univ J Nat Sci. 2019;20(2):129-34.

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