MESNA HAS ANTIOXIDANT POTENTIAL ON INTESTINE, LIVER, KIDNEY AND LUNG INJURY INDUCED BY INTESTINAL ISCHEMIA/REPERFUSION
Abstract
An ischemia/reperfusion (IR) injury of small intestine is a serious and common condition that is a result of the blockage of the superior mesenteric artery (SMA) due to some significant clinical problems. In the present study, we evaluated the effect of Mesna on systemic injury induced by IR in small intestine and liver, kidney and lung of rats. Thirty-two Wistar albino female rats were randomly divided into four groups as control, ischemia, IR (Sham) and IR+Mesna. Ischemia period was executed by clamping SMA for 2 h and after when reperfusion was permitted by removing the clamp from SMA for 2 h. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities were measured spectrophotometrically in the tissues. Microscopic examination was performed with Hematoxylin-Eosin (H&E) staining for light microscopy and double staining (Uranyl acetate and Reynolds’lead citrate) for electron microscopy. Oxidative damage was determined in ischemia (p < 0.05) and IR groups (p < 0.05) by Chiu score in small intestine and also antioxidant enzyme activities in all groups. Significant recovery in SOD, GPx and CAT activities in IR+Mesna group was determined but the effect of oxidative damage was not reduced significantly histopatologically. Mesna treatment caused recovery in SOD, GPx and CAT activities but not achieved an improvement in the histopathologic findings in level of light microscopy in IR damages but contains the signs of improving at electron microscopy level in Mesna dose and application time of IR.
Keywords
mesna ischemia reperfusion SOD Gpx Catalase Electron microscopy
Supporting Institution
Trakya University Research Center
Project Number
TÜBAP 2010-147
Abstract
İnce bağırsağın iskemi/reperfüzyon (İR) hasarı, önemli klinik problemler nedeniyle superior mezenterik arterin (SMA) tıkanmasının bir sonucu olarak ortaya çıkan ciddi ve yaygın bir durumdur. Bu çalışmada, Mesna'nın ince bağırsak, karaciğer, böbrek ve akciğerde İR ile indüklenen sistemik hasar üzerindeki etkisi değerlendirildi. Otuziki Wistar albino dişi sıçan, rastgele dört gruba ayrıldı: Kontrol, iskemi, İR (Sham) ve İR+Mesna. Superior mezenterik arter (SMA) 2 saat klemplenerek iskemi ve ardından klemp SMA'dan çıkarılarak 2 saat reperfüzyon uygulandı. İnce bağırsak, karaciğer, böbrek ve akciğer dokularında, antioksidan enzim aktiviteleri spektrofotometrik olarak ölçüldü. Mikroskobik inceleme için hematoksilen-eozin (H&E), elektron mikroskobik inceleme için çift boyama (uranil asetat ve Reynolds kurşun sitrat) yapıldı. İskemi (p < 0.05) ve IR gruplarında (p < 0.05) ince bağırsakta Chiu skoru ve ayrıca tüm gruplarda antioksidan enzim aktiviteleri ile oksidatif hasar belirlendi. IR+Mesna grubunda SOD, GPx ve CAT aktivitelerinde anlamlı iyileşme saptandı ancak oksidatif hasarın etkisi histopatolojik olarak önemli ölçüde azalmadı. Mesna uygulaması, SOD, GPx ve CAT aktivitelerinde düzelme sağladı ancak histopatolojik bulgularda ışık mikroskobi düzeyinde İR hasarında anlamlı bir iyileşmeye neden olmadı, ancak uygulanan İR süresi ve Mesna dozunda, ultrastrüktürel olarak iyileşme belirtileri içerdi.
Project Number
TÜBAP 2010-147
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Article/Araştırma Makalesi |
| Authors | |
| Project Number | TÜBAP 2010-147 |
| Publication Date | October 15, 2022 |
| Submission Date | June 8, 2022 |
| Acceptance Date | October 3, 2022 |
| Published in Issue | Year 2022 Volume: 23 Issue: 2 |
Cite
You can reach the journal's archive between the years of 2000-2011 via https://dergipark.org.tr/en/pub/trakyafbd/archive (Trakya University Journal of Natural Sciences (=Trakya University Journal of Science)
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