Tuber magnatum Picco: a new record for the Turkish mycobiota

Yıl 2024, Cilt: 25 Sayı: 2, 203 - 210, 15.10.2024

Hasan Hüseyin Doğan , İsmail Şen , Hakan Allı

https://doi.org/10.23902/trkjnat.1475517

Öz

Tuber magnatum Picco, the Italian white truffle mushroom recorded for the first time in Türkiye, is a rare and economically valuable mushroom. Samples were collected from Sakarya province during field studies in 2024. The samples were identified as T. magnatum according to their macro- and microscopic features. To make molecular confirmation of the species, the samples' ITS1 and ITS4 gene regions were analysed and registered in Genbank with the number PP239641. The sample showed 100% compatibility with other T. magnatum accessions in Genbank. The distributions of Tuber species in Türkiye were given, and their taxonomic features were compared.

Anahtar Kelimeler

Ascomycota, Hypogeous fungi, Taxonomy, White truffle, Türkiye

Etik Beyan

The author has no conflicts of interest to declare

Destekleyen Kurum

Selçuk University

Proje Numarası

BAP/20401081

Teşekkür

I would like to thank Ziyafet Arslan for the samples he sent from the Kırklareli region, and Şen Kalyoncu and Onur Özmet for the samples, they sent from the Sakarya region.

Kaynakça

• 1. Akata, I., Sevindik, M. & Şahin, E. 2020. Tuber fulgens Quél. A new record for Turkish truffles. Turkish Journal of Agriculture - Food Science and Technology, 8(11): 2472-2475. https://doi.org/10.24925/turjaf.v8i11.2472-2475.3884
• 2. Akata, I., Şen, İ., Sevindik, M. & Kabaktepe Ş. 2022. Truffle checklist of Türkiye II with a new record. Turkish Journal of Agriculture - Food Science and Technology, 10(10): 1913-1920. https://doi.org/10.24925/turjaf.v10i10.1913-1920.5482
• 3. Alkan, S., Aktaş, S. & Kaşık G. 2018. Türkiye'deki Tuber türleri ve Tuber aestivum için yeni bir lokalite. Selçuk Üniversitesi Fen Fakültesi Dergisi, 44(1): 25-29.
• 4. Alsheikh, A.M. & Trappe, J.M. 1983. Taxonomy of Phaeangium lefebvrei, a desert truffle eaten by birds. Canadian Journal of Botany, 61: 1919-1925.
• 5. Alvarado, P., Moreno, G. & Manjón, J.L. 2012. Comparison between Tuber gennadii and T. oligospermum lineages reveals the existence of the new species T. cistophilum (Tuberaceae, Pezizales). Mycologia, 104: 894-910. https://doi.org/10.3852/11-254
• 6. Assyov, B. & Slavova, M. 2023. Macrofungi in stands of the endemic pine Pinus peuce as inferred from morphological and molecular data. Proceedings of the Bulgarian Academy of Sciences, 76(5): 707-715. https://doi.org/10.7546/CRABS.2023.05.06
• 7. Bonito, G. M., Gryganskyi, A. P., Trappe, J. M. & Vilgalys, R. 2010. A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal. Molecular Ecology, 19(22): 4994-5008. https://doi.org/10.1111/j.1365-294X.2010.04855.x
• 8. Bonito, G., Smith, M. E., Nowak, M., Healy, R. A., Guevara, G., Cazares, E., Kinoshita, A., Nouhra, E. R., Dominguez, L. S., Tedersoo, L., Murat, C., Wang, Y., Moreno, B. A., Pfister, D. H., Nara, K., Zambonelli, A., Trappe, J. M. & Vilgalys, R. 2013. Historical biogeography and diversification of truffles in the Tuberaceae and their newly identified southern hemisphere sister lineage. PLoS One, 8(1): e52765. https://doi.org/10.1371/journal.pone.0052765
• 9. Bonito, G., Trappe, J.M., Donovan, S. & Vilgalys, R. 2011. Improved resolution of major clade within Tuber and taxonomy of species within the Tuber gibbosum complex. Mycologia, 102: 1042-1057. https://doi.org/10.3852/09-213
• 10. Breitenbach, J. & Kränzlin, F. 1983. Fungi of Switzerland: Vol. 1. Ascomycetes. Verlag Mykologia, Luzern, 310 pp.
• 11. Büntgen, U., Lendorff, H., Lendorff, A., Leuchtmann, A., Peter, M., Bagi, I. & Egli, S. 2019. Truffles on the move. Frontiers in Ecology and the Environment, 17: 200-202. https://doi.org/10.1002/fee.2033
• 12. Castellano, M.A. & Türkoğlu, A. 2012. New records of truffle taxa in Tuber and Terfezia from Türkiye. Turkish Journal of Botany, 36: 295-218. https://doi.org/10.3906/bot-1106-10
• 13. Castellano, M.A., Trappe, J.M. & Luoma, D.L. 2004. Sequestrate fungi. In: G.M. Mueller, G.F. Bills and M.S. Foster (eds.). Biodiversity of Fungi: inventory and monitoring methods, Elsevier, Burlington, 197-213.
• 14. Çelik, A., Uzun, Y. & Kaya, A. 2020. Macrofungal biodiversity of Güneysınır district (Konya-Türkiye). Mantar Dergisi, 11(1): 75-83. https://doi.org/10.30708/mantar.662015
• 15. Çevik, F.T., Uzun, Y. & Kaya A. 2021. Ereğli (Konya) yöresinde belirlenen makromantarlar. Mantar Dergisi, 12(2): 138-178. https://doi.org/10.30708/mantar.960666
• 16. Doğan, H.H. 2021. A new truffle species addition, Tuber macrosporum Vittad., to Turkish mycota. Trakya University Journal of Natural Sciences, 22(2): 139-146. https://doi.org/10.23902/trkjnat.873651
• 17. Elliott T.F., Türkoğlu A., Trappe J.M. & Yaratanakul Güngör M. 2016. Turkish truffles 2: eight new records from Anatolia. Mycotaxon, 131: 439-453. https://doi.org/10.5248/131.439
• 18. Fan, L., & Cao, J.Z. 2013. Two new species of white truffle from China. Mycotaxon, 121(1): 297-304.
• 19. Fan, L., Han, L., Zhang, P.R. & Yan, X.Y. 2016. Molecular analysis of Chinese truffles resembling Tuber californicum in morphology reveals a rich pattern of species diversity with emphasis on four new species. Mycologia, 108(2): 344-353.
• 20. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791. https://doi.org/10.2307/2408678
• 21. Flammer, R., Flammer, T. & Reil P. 2013. Les truffes, IHW Verlag. 80 pp.
• 22. Gezer, K., Kaygusuz, O., Çelik, A. & Işıloğlu M. 2014. Ecological characteristics of truffles growing in Denizli province, Türkiye. Journal of Food Agriculture & Environment, 12(2): 1105-1109.
• 23. Gori, L. 2005. Funghi Ipogei Della Lucchesia di Aitre Province Italiane e dall' Estero. Pacini Fazzi, 316 pp.
• 24. Graziosi, S., Hall, I.R. & Zambonelli, A. 2022. The mysteries of the white truffle: Its biology, ecology and cultivation. Encyclopedia, 2: 1959-1971. https://doi.org/10.3390/encyclopedia2040135
• 25. Halász, K., Bratek, Z., Szegő, D., Rudnóy, S., Rácz, I., Lásztity, D. & Trappe, J.M. 2005. Tests of species concepts of the small, white, European group of Tuber spp. based on morphology and rDNA ITS sequences with special reference to Tuber rapaeodorum. Mycological Progress, 4: 281-290.
• 26. Hall, I., Brown, G. & Zambonelli, A. 2008. Taming The Truffle: The History, Lore and Science of The Ultimate Mushroom. Timber Press, Portland, 304 pp.
• 27. Hilszczanska, D., Rosa-Gruszecka, A., Sikora, K. & Szmidla, H. 2014. Soil factors determining the production of Tuber spp. ascocarps in natural stands. Database: http://www.ncbi.nlm.nih.gov/nuccore/KJ524536
• 28. İleri, R., Uzun, Y. & Kaya, A. 2020. Macromycetes of Karadağ (Karaman) and its environs. Mantar Dergisi, 11(1): 57-63. https://doi.org/10.30708/mantar.654111
• 29. Index Fungorum, (2024). https://www.indexfungorum.org/names/Names.asp
• 30. Jeandroz, S., Murat, C., Wang, Y., Bonfante, P & Le Tacon, F. 2008. Molecular phylogeny and historical biogeography of the genus Tuber, the ‘True Truffles’. Journal of Biogeopraphy, 35(5): 815-829. https://doi.org/10.1111/j.1365-2699.2007.01851.x
• 31. Kaya, A., Uzun, Y., Karacan, H.İ. & Yakar, S. 2019. Contributions to the macromycota of Gaziantep province. Kastamonu Universitesi Orman Fakültesi Dergisi, 19(3): 329-341. https://doi.org/10.30616/ajb.749820
• 32. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2): 111-1120. https://doi.org/10.1007/BF01731581
• 33. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096
• 34. Leonardi, M., Iotti, M., Mello, A., Vizzini, A., Paz-Conde, A., Trappe, J. & Pacioni, G. 2021. Typification of the four most investigated and valuable truffles: Tuber aestivum Vittad., T. borchii Vittad., T. magnatum Picco and T. melanosporum Vittad. Cryptogamie Mycologie, 42(9): 149-170. https://doi.org/10.5252/cryptogamie-mycologie2021v42a9
• 35. Lovrić, M., Da Re, D., Vidale, E., Prokofieva, I., Wong, J., Pettenella, D., Verkerk, P.J. & Mavsar, R. 2020. Non-wood forest products in Europe – a quantitative overview. Forest Policy and Economics, 116: 102175. https://doi.org/10.1016/j.forpol.2020.102175
• 36. Marjanović, Ž., Grebenc T., Marković, M., Glišić, A. & Milenković, M. 2010. Ecological specificity and molecular diversity of truffles (Genus Tuber) originating from mid-west of the Balkan Peninsula. Sydowia, 62: 67-87.
• 37. Mello, A., Garnero, L., Meotto, F. & Bonfante, P. 1997. Specific primers for rapid typing of Tuber borchii mycorrhizal roots. Symposium on Plant Biotechnology as a tool for the Exploitation of Mountain Lands, ISHS Acta Horticulturae 457. https://doi.org/10.17660/ActaHortic.1998.457.28
• 38. Mello, A., Vizzini, A., Rollo, F., Bonfante, P. & Trappe, J.M. 2000. Tuber borchii versus Tuber maculatum: neotype studies and DNA analysis. Mycologia, 92: 326-331. https://doi.org/10.2307/3761569
• 39. Montecchi, A. & Sarasini, M. 2000. Funghi Ipogei d'Europa. Associazione Micologica Bresadola, Trento, 714 pp.
• 40. Özderin, S., Yılmaz, F. & Allı, H. 2018. Determining mycorrhiza rate in some oak species inoculated with Tuber aestivum Vittad. (Summer Truffle), Turkish Journal of Forestry, 19(3): 226-232. http://dx.doi.org/10.18182/tjf.435372
• 41. Öztürk, C., Kaşık., G. & Toprak, E. 1997. Ascomycetes makrofunguslarından Türkiye için iki yeni kayıt. Ot Sistematik Botanik Dergisi, 4(1): 53-56.
• 42. Pegler, D.N., Spooner, B.M. & Young, T.W.K. 1993. British Truffles: A revision of British Hypogeous fungi. The Royal Botanic Gardens, Kew, 216 pp.
• 43. Riccioni, C., Rubini, A., Belfiori, B., Gregori, G. & Paolocci, F. 2016. Tuber magnatum: the special one. What makes it so different from other Tuber spp. In: Zambonelli, A., Iotti, M., & Murat, C. (eds.). True truffle (Tuber spp.) in the world. Soil ecology, systematics and biochemistry. Springer, Switzerland. 87-103.
• 44. Riousset, G., Riousset, L., Chevalier, G. & Bardet, M.C. 2012. Truffe d’Europe et de Chine. INRA, Paris.
• 45. Şen, İ., Allı, H. & Civelek, H.S. 2016. Checklist of Turkish truffles. Türk Yaşam Bilimleri Dergisi, 1(2): 103-109.
• 46. Stielow, B., Bratek, Z., Orczán, A.K.I, Rudnóy, S., Hensel, G., Hoffmann, P., Klenk, H.P. & Göker, M. 2011. Species delimitation in taxonomically difficult fungi: the case of Hymenogaster. Plos One, 6(1): e15614. https://doi.org/10.1371/journal.pone.0015614
• 47. Suwannarach, N., Kumla, J., Meerak, J. & Lumyong, S. 2017. Tuber magnatum in Thailand, a first report from Asia. Mycotaxon, 132: 635-642. https://doi.org/10.5248/132.635
• 48. Tamura, K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9: 678-687.
• 49. Tamura, K., Stecher, G. & Kumar, S. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38(7): 3022-3027. https://doi.org/10.1093/molbev/msab120
• 50. Trappe, J.M. & Castellano M.A. 1991. Keys to the genera of truffles (Ascomycetes). McIlvanea, 10: 47-65.
• 51. Trappe, M., Evans, F. & Trappe, J. 2007. Field Guide to North American Truffles: Hunting, Identifying, and Enjoying the World's Most Prized Fungi. Ten Speed Press, Berkeley, Toronto, 136 pp.
• 52. Türkoğlu, A. & Castellano, M.A. 2014. New records of some Ascomycete truffle fungi from Türkiye. Turkish Journal of Botany, 38: 406-416. https://doi.org/10.3906/bot-1303-24
• 53. Türkoğlu, A., Castellano, A.M., Trappe, M.J. & Güngör-Yaratanakul, M. 2015. Turkish truffles I: 18 new records for Türkiye. Turkish Journal of Botany, 39: 359-376. https://doi.org/10.3906/bot-1406-42
• 54. Uzun, Y. & Kaya, A. 2020. The Checklist of the macromycetes determined in Gaziantep province. Anatolian Journal of Botany, 4(2): 106-115. https://doi.org/10.18016/ksutarimdoga.vi.856014
• 55. Uzun, Y. & Yakar, S. 2018. New locality record for two Tuber species in Türkiye. Anatolian Journal of Botany, 2(2): 88-92. https://doi.org/10.18016/ksutarimdoga.vi.857201
• 56. Wedén, C., Pettersson, L. & Danell, E. 2009. Truffle cultivation in Sweden: Results from Quercus robur and Corylus avellana field trials on the island of Gotland. Scandinavian Journal of Forest Research, 24(1): 37-53. https://doi.org/10.1080/02827580802562056
• 57. White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322. In: Innis M.A., Gefland D.H., Sninsky J.J. & White T.J. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., New York, 482 pp.