Yıl 2019, Cilt 11 , Sayı 1, Sayfalar 153 - 162 2019-01-31

Investigation of the optimum conditions of immobilized and free laccase enzyme and its use in decolorization process
İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı

Haydar Altınok [1]


In this study, Laccase enzyme (L) from Tramatesversicolor was entrapped into poly(N-isopropylacryl amide)-calcium alginate (P(NIPA)-CaAlj)  beads The properties of the immobilized enzyme and use of enzymatic decolorization processes were investigated and compared with those of the free enzyme. Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) values were found to be 1.70x10-2 mM and 2.08x10-3 mM.min-1 for free enzyme respectively. Km and Vmax values were found as 4.80x10-2 mM and 8.70 x 10-3 mM.min-1 for entrapped enzymes respectively. Optimum pH was determined as 5.0 and 6.0 and optimum temperature determined as 40oC and 45oC for free laccase and entrapped laccase respectively. After 30 days of storage at 4 oC free laccase retained 60 % of its original activity. Also after 30 days of storage at 4 oC, entrapped enzymes were retained 83 % its original activity. Immobilized enzyme was used repeatedly 10 times, were retained 77 % of its original activities. Percent decolorization of methyl orange by free enyzme and entrapped enyzmes were found to be 73% and 70%, respectively. 

Bu çalışmada Tramates versicolor dan elde edilen Laccase enzimi (L), poli(N-izopropilakrilamit)-kalsiyum aljinat (P(NIPA)-CaAlj) taneciklerine hapsetme yöntemi ile immobilize edildi. İmmobilize enzimin özellikleri ve enzimatik renk giderme işlemlerinde kullanımı araştırıldı ve serbest enzim ile karşılaştırıldı.  Michaelis-Menten sabiti (Km) ve maksimum reaksiyon hızı (Vmax) değerleri sırasıyla serbest enzim için 1,70x10-2 mM ve 2,08x10-3 mM.dak-1 olarak bulundu. İmmobilize enzim için Km ve Vmax değerleri de sırasıyla 4,80x10-2 mM ve 8,70 x 10-3 mM.dak-1 olarak bulundu. Optimum pH değerleri serbest enzim için 5,0 ve immobilize enzim için 6,0 olarak belirlendi. Optimum sıcaklık sırasıyla serbest lakkaz ve immobilize lakkaz için 40oC ve 45oC olarak belirlendi. 4 oC tutulan serbest lakkazın 30 günlük depolama sonrasında orijinal aktivitesinin % 60'ı koruduğu bulunurken aynı koşullarda tutulan immobilize enzimin ise orijinal aktivitesinin% 83’ ünü koruduğu bulundu. İmmobilize enzimin 10 kez tekrar kullanım sonrasında orijinal aktivitesinin % 77'sini koruduğu bulunmuştur. Metil oranjın renginin giderilmesinde, serbest lakkaz ve immobilize lakkaz için renk giderme yüzdeleri sırasıyla %73 ve %70 olarak bulundu

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Birincil Dil tr
Konular Mühendislik, Ortak Disiplinler
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-0043-9385
Yazar: Haydar Altınok (Sorumlu Yazar)
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 31 Ocak 2019

Bibtex @araştırma makalesi { umagd508095, journal = {International Journal of Engineering Research and Development}, issn = {}, eissn = {1308-5514}, address = {Kırıkkale Üniversitesi Mühendislik Fakültesi Dekanlığı Kampüs 71450 Yahşihan/KIRIKKALE}, publisher = {Kırıkkale Üniversitesi}, year = {2019}, volume = {11}, pages = {153 - 162}, doi = {10.29137/umagd.508095}, title = {İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı}, key = {cite}, author = {Altınok, Haydar} }
APA Altınok, H . (2019). İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı. International Journal of Engineering Research and Development , 11 (1) , 153-162 . DOI: 10.29137/umagd.508095
MLA Altınok, H . "İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı". International Journal of Engineering Research and Development 11 (2019 ): 153-162 <https://dergipark.org.tr/tr/pub/umagd/issue/39915/508095>
Chicago Altınok, H . "İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı". International Journal of Engineering Research and Development 11 (2019 ): 153-162
RIS TY - JOUR T1 - İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı AU - Haydar Altınok Y1 - 2019 PY - 2019 N1 - doi: 10.29137/umagd.508095 DO - 10.29137/umagd.508095 T2 - International Journal of Engineering Research and Development JF - Journal JO - JOR SP - 153 EP - 162 VL - 11 IS - 1 SN - -1308-5514 M3 - doi: 10.29137/umagd.508095 UR - https://doi.org/10.29137/umagd.508095 Y2 - 2019 ER -
EndNote %0 Uluslararası Mühendislik Araştırma ve Geliştirme Dergisi İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı %A Haydar Altınok %T İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı %D 2019 %J International Journal of Engineering Research and Development %P -1308-5514 %V 11 %N 1 %R doi: 10.29137/umagd.508095 %U 10.29137/umagd.508095
ISNAD Altınok, Haydar . "İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı". International Journal of Engineering Research and Development 11 / 1 (Ocak 2019): 153-162 . https://doi.org/10.29137/umagd.508095
AMA Altınok H . İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı. IJERAD. 2019; 11(1): 153-162.
Vancouver Altınok H . İmmobilize ve Serbest Lakkaz Enziminin Optimum Koşullarının Araştırılması ve Renk Giderme İşleminde Kullanımı. International Journal of Engineering Research and Development. 2019; 11(1): 162-153.