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Van Sağlık Bilimleri Dergisi

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Van Yüzüncü Yıl Üniversitesi

Clinical Sciences

Klinik Tıp Bilimleri

GLİOBLASTOMA’da 5-FU ve CELASTROL’ün SİTOTOKSİK ETKİSİ

THE CYTOTOXIC EFFECT of 5-FU AND CELASTRO L on GLIOBLASTO MA

Taşpınar

Mehmet

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI Denizler

Farika Nur

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI Güven

Mustafa

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, TIP FAKÜLTESİ Yüksek

Veysel

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, ÖZALP MESLEK YÜKSEKOKULU Çetin

Sedat

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, TEMEL BİLİMLER BÖLÜMÜ, VETERİNERLİK BİYOKİMYASI ANABİLİM DALI Dede

Semiha

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, TEMEL BİLİMLER BÖLÜMÜ, VETERİNERLİK BİYOKİMYASI ANABİLİM DALI

07 08 2019

11 1 6 10 11 10 2018 12 12 2018

2018

Van Sağlık Bilimleri Dergisi

Glioblastoma (GB), enmalign ve en agresif beyin tümörüdür.Fenotipik ve genotipik açıdan yüksek heterojeniteye sahip Glioblastomanın kesin bir tedavi şekli bulunmamaktadır. Glioblastomadakemoterapi uygulamaları tedavide önemlibir yer tutmaktadır. Ancak kemoterapötik direnç tedavi başarısını düşürmektedir. Kemoterapötik direnci azaltmak ve etkili birkemoterapi iç in bitkiselköken li antikanserojenik mo le küllereliştirilmeye çalışılmaktadır. Son yıllarda, önemlibir tedavi yaklaşımı olara k, bu mo leküllerin tek uygulanmalarının yanında kemoterapötik ajanlarla kombine uygulamaları artmaktadır. Bu çalış mada,kemoterapötik ajan 5-Fluorourac il ile doğal birbileşik olan Ce lastrol kombinasyonunun Glioblastomadaki sitotoksik etkisinin araştırılması amaç lan mıştır.MTT sitotoksisite testi sonucunda 2 µM Celastrolün, 50 µM 5-Fluorouracilin sitotoksik etkisini % 19 oranında arttırdığı, 0,5 ve 2 µM’lık Celastrolün100 µM’lık 5-Fluorouracilin sitotoksik etkisini sırasıyla% 25 ve %37 oranında arttırdığı saptanmıştır. Bu çalış ma, tüm kansertiplerinde 5-Fluorouracilin ile Celastrolün kombine etkisinin gösterildiğ i ilkçalış madır. Celastrolün 5-Fluorouracil ile birlikte kullanımının önemini ilkkez gösteren bu çalışma, yeni bir tedavi protokülünün geliştirilmesi amacıyladaha ileri düzey araştırmalara temel bir dayanak oluşturabilir.

Glioblastoma is the mostmalign and agressive brain tumor. Glioblastomahas heterogeneityin terms of genotypicallyand phenotypically and alsothere is no precise treatment model. Chemotherapy has a bigrole in the treatment of glioblastoma but chemotherapeutic resistancedecrease the effectiveness of treatment.Anticancerogenic molecules whichderived from plants are devoloping withthe purpose of decreasing chemotheraupatic resistance and effective treatment. In recent years, as animportant treatment approach, the combination of these molecules withchemotherapeutic agents as well as single administration increases. In this study, it was aimed to investigate the cytotoxic effect of the combination of chemotherapeuticagent 5- Fluorouracil and a natural compound Celastrol in glioblastoma.As a result of the MTT cytotoxicity test, it wasdetermined that Celastrol (2µM) has increased the cytotoxic effect of 5- Fluorouracil(50 µM) by 19%, the cytotoxic effect of 5-Fluorouracil (100 µM) has increasedby celastrol (0.5 and 2 µM) inratio of 25% and 37% , respectively.This is the first studyto show the effects of 5-Fluorouracil and celastrol combinationin alltypes of cancer. This study, whichdemonstrated the importance of the use ofcelastrol with 5-Fluorouracil fo r the first time, may provide a basis for further research in order to develop a new treatment protocol

5-Florourasil Celastrol Glioblastoma Sitotoksisite

5-Fluorouracil Celastrol Glioblastoma Cytotoxicity

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