The effect of resveratrol on live weight, serum biochemistry and tissue antioxidant enzymes in rats

Abstract

The aim of this study is to determine the effects of resveratrol used in adding it to drinking water. The study was carried out with 24 Wistar albino male 20 days rats for 40 days. The study design was determined as one control and two trial groups (each group with 2 parallels and each parallel with 4 rats). The first group was given a basal ration and drinking water. The second group was given the basal ration and drinking water with 10 mg/kg resveratrol; and the third group was given the basal ration and drinking water 20 mg/kg resveratrol. In serum biochemistry parameters, significant decreases were observed in serum Aspartate aminotransferase (AST) enzyme activity, blood urea nitrogen (BUN), albumin, total protein and globulin concentrations and increase in albumin/globulin ratio in groups given resveratrol (p<0.05). Regardless of the dose, an increase in the concentration of glutathione (GSH) from the antioxidant parameters was observed in the liver; In the kidney, an increase was observed in the Res10 group. In addition, a decrease in muscle superoxide dismutase (SOD) activity and an increase in muscle and kidney catalase (CAT) activity were observed. A decrease was observed in malondialdehyde (MDA) concentrations, which is a parameter of lipid peroxidation in the kidney. As a result, it was determined in the present study that resveratrol has antioxidant effects regardless of the way of use, and it was concluded that this additive can be used for various purposes.

Keywords

• Antioxidant
• serum biochemistry
• resveratrol
• rat
• water

References

1. Alema´n, C.L., Ma´s, R.M., Rodeiro, I., Noa, M., Herna´ndez, C., Mene´ndez, R., Ga´mez, R. (1998). Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months. Laboratory Animals, 32(4), 457–466. https://doi.org/10.1258/002367798780599802
2. Badgujar, P.C., Pawar, N.N., Chandratre, G.A., Telang, A.G., Sharma, A.K. (2015). Fipronil induced oxidative stress in kidney and brain of mice: protective effect of vitamin E and vitamin C. Pesticide Biochemistry and Physiology, 118, 10-18. https://doi.org/10.1016/j.pestbp.2014.10.013
3. Baxter, R.A. (2008). Anti-aging properties of resveratrol: Review and report of a potent new antioxidant skin care formulation. Journal of Cosmetic Dermatology, 7, 2–7.
4. Bhatt, J.K., Thomas, S., Nanjan, M.J. (2012). Resveratrol supplementation improves glycemic control in type 2 diabetes mellitus. Nutrition Research, 32(7), 537-541. https://doi.org/10.1016/j.nutres.2012.06.003
5. Carbo´, N., Costelli, P., Baccino, F.M., Lo´pez-Soriano, F.J., Argile´s, J.M. (1999). Resveratrol, a natural product present in wine, decreases tumor growth in a rat tumor model. Biochemical and Biophysical Research Communications, 254(3), 739–743. https://doi.org/10.1006/bbrc.1998.9916
6. Chand, N., Naz, S., Rehman, Z., Khan, R.U. (2018). Blood biochemical profile of four fast-growing broiler strains under high ambient temperature. Applied Biological Chemistry, 61(3), 273-279. https://doi.org/10.1007/s13765-018-0358-4
7. Comba, B., Cinar, A., Comba, A., Gencer, Y.G. (2016). Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 229-233.
8. Dal-Pan, A., Blanc, S., Aujard, F. (2010). Resveratrol suppresses body mass gain in a seasonal non-human primate model of obesity. BMC Physiology, 10(11). https://doi.org/10.1186/1472-6793-10-11

9. Frankel, E.N., Waterhouse, A.L., Kinsella, J.E. (1993). Inhibition of human LDL oxidation by resveratrol. Lancet, 341(8852), 1103-1104. https://doi.org/10.1016/0140-6736(93)92472-6
10. Ghanim, H., Sia, C.L., Abuaysheh, S., Korzeniewski, K., Patnaik, P., Marumganti, A., Chaudhuri, A., Dandona, P. (2010). An antiinflammatory and reactive oxygen species suppressive effect of an extract of Polygonum cuspidatum containing resveratrol. Journal of Clinical Endocrinology and Metabolism, 95(9), E1-E8. https://doi.org/10.1210/jc.2010-0482
11. Ghasemi, H.A., Nari, N. (2020). Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels. Journal of Applied Poultry Research, 29(2), 301-313. https://doi.org/10.1016/j.japr.2019.11.004
12. Harada, N., Zhao, J., Kurihara, H., Nakagata, N., Okajima, K. (2011). Resveratrol improves cognitivefunction in mice by increasing production of insulin-like growth factor-I in the hippocampus. Journal of Nutritional Biochemistry, 22(12), 1150-1159. https://doi.org/10.1016/j.jnutbio.2010.09.016 Hausenblas, H.A., Schoulda, J.A., Smoliga, J.M., Schoulda, S. (2014). Resveratrol treatment as an adjunct to pharmacological management in type 2 diabetes mellitus-systematic review and meta-analysis. Molecular Nutrition and Food Research, 59(1), 147-159. https://doi.org/10.1002/mnfr.201400173 He, S., Li, S., Arowolo, M.A., Yu, Q., Chen, F., Hu, R., He, J. (2019). Effect of resveratrol on growth performance; rectal temperature and serum parameters of yellow‐feather broilers under heat stress. Animal Science Journal, 90(3), 401-411. https://doi.org/10.1111/asj.13161
13. Helkin, A., Stein, J.J., Lin, S., Siddiqui, S., Maier, K.G., Gahtan, V. (2016). Dyslipidemia part 1-review of lipid metabolism and vascular cell physiology. Vascular and Endovascular Surgery, 50(2), 107-118. https://doi.org/10.1177/1538574416628654
14. Hong, H.J., Kang, W., Kim, D.G., Lee, D.H., Lee, Y., Han, C. (2014). Effects of resveratrol on the insulin signaling pathway of obese mice. Journal of Veterinary Science, 15(2), 179-185. https://doi.org/10.4142/jvs.2014.15.2.179
15. Hu, H., Bai, X., Xu, K., Zhang, C., Chen, L. (2021). Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers. Poultry Science 100(8), 101217. https://doi.org/10.1016/j.psj.2021.101217
16. Jiang, Z., Wan, Y., Li, P., Xue, Y., Cui, W., Chen, Q., Mao, D. (2019). Effect of curcumin supplement in summer diet on blood metabolites, antioxidant status, immune response, and testicular gene expression in Hu sheep. Animals, 9(10), 720. https://doi.org/10.3390/ani9100720
17. Joe, A.K., Liu, H., Suzui, M., Vural, M.E., Xiao, D., Weinstein, I.B. (2002). Resveratrol induces growth inhibition, S-phase arrest, apoptosis, and changes in biomarker expression in several human cancer cell lines. Clinical Cancer Research, 8(3), 893–903.
18. Juan, M.E., Vinardell, M.P., Planas, J.M. (2002). The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful. Journal of Nutrition, 132, 257-260. https://doi.org/10.1093/jn/132.2.257
19. Khan, M.A., Chen, H.C., Wan, X.X., Tania, M., Xu, A.H., Chen, F.Z., Zhang, D.Z. (2013). Regulatory effects of resveratrol on antioxidant enzymes: A mechanism of growth inhibition and apoptosis induction in cancer cells. Molecular Cell, 35(3), 219-225. https://doi.org/10.1007/s10059-013-2259-z
20. Lee, E.S., Shin, M.O., Yoon, S., Moon, J.O. (2010). Resveratrol inhibits dimethylnitrosamine-induced hepatic fibrosis in rats. Archives of Pharmacal Research, 33, 925-932. https://doi.org/10.1007/s12272-010-0616-4
21. Liu, G.S., Zhang, Z.S., Yang, B., He, W. (2012). Resveratrol attenuates oxidative damage and ameliorates cognitive impairment in the brain of senescence-accelerated mice. Life Science, 91(17-18), 872–877. https://doi.org/10.1016/j.lfs.2012.08.033
22. Liu, L.L., He, J.H., Xie, H.B., Yang, Y.S., Li, J.C., Zou, Y. (2014). Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens. Poultry Science, 93(1), 54-62. https://doi.org/10.3382/ps.2013-03423
23. Moreno, M., Castro, E., Falqué, E. (2008). Evolution of trans- and cis- resveratrol content in red grapes (Vitis vinifera L. cv Menciá; Albarello and Merenzao) during ripening. European Food Research and Technology, 227(3), 667-674. https://doi.org/10.1007/s00217-007-0770-1
24. Mossa, A.T.H., Swelam, E.S., Mohafrash, S.M. (2015). Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histopathological changes in the liver and kidney of male albino rats. Toxicology Reports, 2, 775-784. https://doi.org/10.1016/j.toxrep.2015.02.009
25. Nigdikar, S.V., Williams, N.R., Griffin, B.A., Howard, A.N. (1998). Consumption of red wine polyphenols reduces the susceptibility of low-density lipoproteins to oxidation in vivo. American Journal of Clinical Nutrition, 68(2), 258-265. https://doi.org/10.1093/ajcn/68.2.258
26. Nosáľ, R., Drábiková, K., Jančinová, V., Perečko, T., Ambrožová, G., Číž, M., Lojek, A., Pekarová, M., Šmidrkal, J., Harmatha, J. (2014). On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes. Oxidative Medicine and Cellular Longevity, 2014, 706269. https://doi.org/10.1155/2014/706269
27. NRC (1994). Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC. USA.
28. OECD (Organization for Economic Cooperation and Development). (1995). Guidelines for testing chemicals. Repeated dose 28-d oral toxicity study in rodents, No. 407. Paris, France.
29. Ölmez, M., Şahin, T., Makav, M., Karadağoğlu, Ö. (2020). Effect of resveratrol supplemented to japanese quail (Coturnix coturnix japonica) rations on performance and some biochemical parameters. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(6), 807-812. https://doi.org/10.9775/kvfd.2020.24542
30. Pallauf, K., Chin, D., Gunther, I., Birringer, M., Luersen, K., Schultheiss, G., Vieten, S., Krauss, J., Bracher, F., Danylec, N., Soukop, S.T., Kulling, S.E., Rimbach, G. (2019). Resveratrol, lunularin and dihydroresveratrol do not act as caloric restriction mimetics when administered intraperitoneally in mice. Scientific Reports, 9(1), 4445. https://doi.org/10.1038/s41598-019-41050-2
31. Poulsen, M.M., Jorgensen, J.O., Jessen, N., Richelsen, B., Pedersen, S.B. (2013). Resveratrol in metabolic health: An overview of the current evidence and perspectives. Annals of the New York Academy of Sciences, 1290(1), 74-82. https://doi.org/10.1111/ nyas.12141
32. Rasković, A., Ćućuz, V., Torović, L., Tomas, A., Gojković-Bukarica, L., Ćebović, T., Milijašević, B., Stilinović, N., Cvejić Hogervorst, J. (2019). Resveratrol supplementation improves metabolic control in rats with induced hyperlipidemia and type 2 diabetes. Saudi Pharmaceutical Journal, 27(7), 1036–1043. https://doi.org/10.1016/j.jsps.2019.08.006
33. Shankar, S., Singh, G., Srivastava, R.K. (2007). Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential. Frontiers in Bioscience – Landmark, 12(12), 4839-4854. https://doi.org/10.2741/2432
34. Singh, C.K., Ndiaye, M.A., Ahmad, N. (2015). Resveratrol and cancer: Challenges for clinical translation. Biochimica et Biophysica Acta (BBA), 1852(16), 1178-1185. https://doi.org/10.1016/j.bbadis.2014.11.004
35. Smoliga, J.M., Sage, C.E., Campen, M.J. (2013). A healthier approach to clinical trials evaluating resveratrol for primary prevention of age-related diseases in healthy populations. Aging, 5(7), 495-506. https://doi.org/10.18632/aging.100579
36. Sridhar, M., Suganthi, R.U., Thammaiah, V. (2015). Effect of dietary resveratrol in ameliorating aflatoxin B1-induced changes in broiler birds. Journal of Animal Physiology and Animal Nutrition, 99(6), 1094-1104. https://doi.org/10.1111/jpn.12260
37. Stivala, L., Savio, M., Carafoli, F., Perucca, P. (2001). Specific structural determinants are responsible for the antioxidant activity and the cell cycle effects of resveratrol. Journal of Biological Chemistry, 276(25), 22586-22594. https://doi.org/10.1074/jbc.M101846200
38. Szkudelska, K., Nogowski, L., Szkudelski, T. (2009). Resveratrol, a naturally occurring diphenolic compound, affects lipogenesis, lipolysis and the antilipolytic action of insulin in isolated rat adipocytes. Journal of Steroid Biochemistry and Molecular Biology, 113(1-2), 17-24. https://doi.org/10.1016/j.jsbmb.2008.11.001
39. Szkudelski, T. and Szkudelska, K. (2015). Resveratrol and diabetes: From animal to human studies. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1852(6), 1145-1154. https://doi.org/10.1016/j.bbadis.2014.10.013
40. Şahin, K., Akdemir, F., Orhan, C., Tuzcu, M., Hayirli, A., Şahin, N. (2010). Effects of dietary resveratrol supplementation on egg production and antioxidant status. Poultry Science, 89(6), 1190-1198. https://doi.org/10.3382/ps.2010-00635
41. Şahin, K., Orhan, C., Akdemir, F., Tuzcu, M., Iben, C., Şahin, N. (2012). Resveratrol protects quail hepatocytes against heat stress: Modulation of the Nrf2 transcription factor and heat shock proteins. Journal of Animal Physiology and Animal Nutrition, 96(1), 66-74. https://doi.org/10.1111/j.1439-0396.2010.01123.x
42. Turner, R.T., Evans, G.L., Zhang, M., Maran, A., Sibonga, J.D. (1999). Is resveratrol an estrogen agonist in growing rats? Endocrinology, 140(1), 50–54. https://doi.org/10.1210/endo.140.1.6460
43. Upadhyay, G., Singh, A.K., Kumar, A., Prakash, O., Singh, M.P. (2008). Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. European Journal of Pharmacology, 596(1-3), 146-152. https://doi.org/10.1016/j.ejphar.2008.08.019
44. Wilson, T., Knight, T.J., Beitz, D.C., Lewis, D.S., Engen, R.L. (1996). Resveratrol promotes atherosclerosis in hypercholesterolemic rabbits. Life Science, 59(1), 15–21. https://doi.org/10.1016/0024-3205(96)00260-3
45. Xia, N., Daiber, A., Förstermann, U., Li, H. (2017). Antioxidant effects of resveratrol in the cardiovascular system. British Journal of Pharmacology, 174(12), 1633-1646. https://doi.org/10.1111/bph.13492
46. Xie, H.C., Han, H.P., Chen, Z., He, J.P. (2013). A study on the effect of resveratrol on lipid metabolism in hyperlipidemic mice. African Journal of Traditional, 11(1), 209-212.
47. Zhang, Z., Gao, L., Cheng, Y., Jiang, J., Chen, Y., Jiang, H., Yu, H., Shan, A., Cheng, B. (2014). Resveratrol; a natural antioxidant; has a protective effect on liver injury induced by inorganic arsenic exposure. BioMed Research International, 2014, 1-7. https://doi.org/10.1155/2014/617202
48. Zhang, C., Zhao, X., Wang, L., Yang, L., Chen, X., Geng, Z. (2017). Resveratrol beneficially affects meat quality of heat-stressed broilers which is associated with changes in muscle antioxidant status. Animal Science Journal, 88(10), 1569-1574. https://doi.org/10.1111/asj.12812
49. Zhang, C., Yang, L., Zhao, X., Chen, X., Wang, L., Geng, Z. (2018). Effect of dietary resveratrol supplementation on meat quality, muscle antioxidative capacity and mitochondrial biogenesis of broilers. Journal of the Science of Food and Agriculture, 98(3), 1216-1221. https://doi.org/10.1002/jsfa.8576