Determination of antibiotic resistance profiles of Bacillus cereus from subclinical mastitis of cattle in İzmir province

Çağatay Nuhay; Deha Ali Deniz; Volkan Özavcı

doi:10.31797/vetbio.1620730

Determination of antibiotic resistance profiles of Bacillus cereus from subclinical mastitis of cattle in İzmir province

Abstract

Mastitis causes significant economic losses in the dairy industry. In Türkiye, an estimated annual economic loss of 41.5 million TL is attributed to mastitis. Environmental and milking factors, alongside microbial factors, play a role in the development of mastitis. Various microorganisms, primarily bacteria, cause mastitis. This study aimed to determine the presence of Bacillus cereus and their antimicrobial susceptibilities in milk samples from subclinically infected cows in Izmir province. Milk samples from 400 dairy cows in Izmir province with subclinical mastitis were brought to the Bornova Veterinary Control Institute Bacteriology Laboratory and cultured on Columbia Agar supplemented with 5% sheep blood under cold chain conditions. Gram-positive bacilli colonies were purified and identified using the BCL prepared diagnostic kit on the VITEK 2 (bioMérieux) device. Five B. cereus (1.25 %) were isolated and identified from 400 subclinical mastitis milk samples by VITEK and PCR. The isolates were sensitive to gentamicin, chloramphenicol and tetracycline at the rates of 100 %. All of the isolates were resistant to amoxicillin clavulanic acid. Three of the isolates (60 %) were resistant against trimethoprim-sulfamethoxazole. The results suggest that Bacillus cereus may be responsible for subclinical mastitis cases in cattle in terms of public health, and that specidic treatment should be administered considering the antimicrobial susceptibility data obtained in our study.

Keywords

References

Ağaoğlu S., Alemdar S., Ekici K., & Güdücüoğlu H. (1999). Çiğ süt ve bazı süt ürünlerinde Bacillus cereus’un varlığının araştırılması. Yüzüncü Yıl Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisi, 9(1), 5-7.
Ajose, D. J., Oluwarinde, B. O., Abolarinwa, T. O., Montso, K. P., Fri, J., Aremu, A. O., & Ateba, C. N. (2022). Combating bovine mastitis in the dairy sector in an era of antimicrobial resistance: Ethnoveterinary medicinal option as a viable alternative approach. Frontiers in Veterinary Science, 287. https://doi.org/10.3389/fvets.2022.800322
Arslan, E., Celebi, A., Acik, L., & Uçan, U. S. (2009). Characterisation of coagulase positive staphylococcus species isolated from bovine mastitis using protein and plasmid determination of antibiotic. Turkish Journal of Veterinary and Animal Sciences, 33(6), 493-500. https://doi.org/10.3906/vet-0808-8
Ateş, M., Erganiş, O., Kaya, O., & Çorlu, M. (1990). Koyunlarda mastitis üzerine mikrobiyolojik araştırmalar. Avrasya Veteriner Bilimleri Dergisi, 6(1), 3-6.
Atyabi N., Vodjgani B., Gharagozloo F., & Bahonar A., (2006). Prevalence of bacterial mastitis in cattle from the farms around Tehran. Iran Journal of Veterinary Research University of Shiraz, 3(16), 76-79. https://doi.org/10.22099/ijvr.2006.2654
Brown, R.W. & Scherer, R.K. (1957): A report on two cases of acute mastitis caused by Bacillus cereus. Cornell Veterinarian: 47(2), 226-40.
Clinical and Laboratory Standards Institute. (2012). Performance standards for antimicrobial susceptibility testing (12th ed.). Clinical and Laboratory Standards Institute.
Clinical and Laboratory Standards Institute. (2020). Performance standards for antimicrobial susceptibility testing (30th ed.). CLSI Supplement M100. Clinical and Laboratory Standards Institute.

Eid R.H., Aref N.E., & Ibrahim E.S. (2023) Phenotypic diagnosis and genotypic identification of Bacillus cereus causing subclinical mastitis in cows, Veterinary World, 16(5), 888-894. https:// doi.org/10.14202/vetworld.2023.888-894
Erdoğdu, M. (2019). Mastitisli sütlerden Klebsiella spp. izolasyonu ve antibiyotik dirençliliğinin belirlenmesi. (Tez no: 536520) [Yüksek lisans tezi, Aydın Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü)]. Ulusal Tez Merkezi
Erer, H., Ateş, M., Kaya, O., Kıran, M. M., & Berkin, Ş. (1990). Koyun mastitisleri üzerinde patolojik ve bakteriyolojik incelemeler. Etlik Veteriner Mikrobiyoloji Dergisi, 6(6), 79-97.
Gazali M.F., Sukiman M.Z., Çay M.H., Muhammed N.M., & Ariffin B. Z. (2022). Molecular detection and antibiogram of Bacillus cereus ısolated from dairy goat with mastitis in Malaysia. International Journal of Infectious Diseases, 116, 63-64. https://doi.org/10.1016/j.ijid.2021.12.149
Halasa, T., Huijps, K., Osteras, O., & Hogeveen, H. (2007). Economic effects of bovine mastitis and mastitis management: A review. Veterinary Quarterly, 29(1), 18-31. https://doi.org/10.1080/01652176.2007.9695224
Halket, G., Dinsdale, A. E., & Logan, N. A. (2010). Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers. Letters in Applied Microbiology, 50(1), 120-126. https://doi.org/10.1111/j.1472-765X.2009.02765.x
Jung J., Jin H., & Seo S. (2022). Enterotoxin genes and antibiotic susceptibility of Bacillus cereus isolated from Garlic chives and agricultural environment. International Journal Environmental Research Public Health, 19(19), 12159. https://doi.org/10.3390/ijerph191912159
Kalaylı, E., & Beyatlı, Y. (2003). Bacillus cinsi bakterilerin antimikrobiyal aktiviteleri, PHB üretimleri ve plazmid DNA’ları. Orlab On-Line Mikrobiyoloji Dergisi, 1(12), 24-35.
Kırkan, Ş., Göksoy, E. Ö., & Kaya, O. (2005). Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative Staphylococci from bovine mastitis in the Aydın region of Türkiye. Turkish Journal of Veterinary and Animal Sciences, 29(3), 791-796.
Nuhay, Ç. (2025). Investigation of the Presence and Antimicrobial Susceptibilities of Corynebacterium pseudotuberculosis in Subclinical Mastitis in Cattle Raised in the Aegean Region. Journal of Anatolian Environmental and Animal Sciences, 10(2), 202-207. https://doi.org/10.35229/jaes.1629149
Okulmuş, Ç., Akçay, H., İçil, N. İ., Türkyılmaz, Ö. (2023). The effect of different particle sized rations and live yeast supplementation on energy profile in dairy cows in heat stress. Etlik Veteriner Mikrobiyoloji Dergisi, 34(1), 30-35. https://doi.org/10.35864/evmd.1257761
Oliwa‐Stasiak, K., Molnar, C. I., Arshak, K., Bartoszcze, M., & Adley, C. C. (2010). Development of a PCR assay for identification of the Bacillus cereus group species. Journal of Applied Microbiology, 108(1), 266-273. https://doi.org/10.1111/j.1365-2672.2009.04419.x
Pichhardt, K. (2004). Gıda mikrobiyolojisi. Gıda endüstrisi için temel esaslar ve uygulamalar 4th ed.) Çeviri. İstanbul Literatür Yayıncılık.
Sabuncuoğlu, N., & Çoban, Ö. (2010). Mastitis ekonomisi. Atatürk Üniversitesi Veteriner Bilimleri. Dergisi, 1 (1-2), 1-5
Savaşan, S., Nuhay, Ç., Ergüden, V. E., & Savaşan, S. (2023). Determination of biofilm formation, antibacterial resistance and genotypes of Bacillus cereus isolates from raw milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 29(3), 265-271. https://doi.org/10.9775/kvfd.2023.29162
Schwarz, S., Loeffler, A., & Kadlec, K. (2017). Antimicrobial resistance in livestock. Animal Health Research Reviews, 18(1), 19-29. https://doi.org/10.1111/vde.12362
Tel, O. Y., Keskin, O., Zonturlu, A. K., & Kaya, N. B. A. (2009). Şanlıurfa yöresinde subklinik mastitislerin görülme oranı, aerobik bakteri izolasyonu ve duyarlı antibiyotiklerin belirlenmesi. Fırat üniversitesi Sağlık Bilimleri Veteriner Dergisi, 23(2), 101-106.
Uçan, U. S., Açik, L., Çelebi, A., Erganiş, o., & Arslan, E. (2005). Plasmids and protein patterns of Escherichia coli isolated from bovine mastitis in Konya, Türkiye. Turkish Journal of Veterinary and Animal Sciences, 29(2), 475-480.
Yalçın, S., Çöl, B. G., & Aksu, H. (2023). Bitkisel kaynaklı gıdalardan izole edilen B. cereus suşlarının bazı antibiyotiklere karşı duyarlılık profillerinin belirlenmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 16(2), 75-79. https://doi.org/10.47027/duvetfd.1298336
Yumuşak, M., & Küçükayan, U. (1995). Ankara bölgesindeki mastitisli inek sütlerinde Bacillus cereus aranması. Etlik Veteriner Mikrobiyoloji Dergisi, 8(1), 198-217.

Details

Primary Language

English

Subjects

Veterinary Bacteriology

Journal Section

Research Article

Authors

Çağatay Nuhay
0000-0002-1475-3041
Türkiye

Deha Ali Deniz ^*
0000-0002-7885-9523
Türkiye

Volkan Özavcı
0000-0003-3511-3008
Türkiye

Publication Date

August 31, 2025

Submission Date

January 15, 2025

Acceptance Date

April 24, 2025

Published in Issue

Year 2025 Volume: 10 Number: 2

DOI

https://doi.org/10.31797/vetbio.1620730

IZ

https://izlik.org/JA93HD22SS

Cite

RIS / Bibtex

APA

Nuhay, Ç., Deniz, D. A., & Özavcı, V. (2025). Determination of antibiotic resistance profiles of Bacillus cereus from subclinical mastitis of cattle in İzmir province. Journal of Advances in VetBio Science and Techniques, 10(2), 88-91. https://doi.org/10.31797/vetbio.1620730

Cited By

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Harran Üniversitesi Veteriner Fakültesi Dergisi

https://doi.org/10.31196/huvfd.1791793