Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes.

Ebru Afşar; Işıl Eranıl; Erkan Özkan; Erdem Aslan; Kadirhan Doğan; Mehmet Öz

doi:10.31797/vetbio.1655445

Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes.

Abstract

Background: The present study aimed to assess the effect of Porphyromonas gingivalis-Lipopolysaccharide (P.gingivalis-LPS) on hepatic disorder mediated by 5-hydroxytryptamine receptor 2A (HT2AR) and whether there is any impact of Thymoquinone (TQ) administration. Methods: 10 µl P. gingivalis-LPS (1mg/ml) injected 6 times at 48-hour intervals into the palatal gingiva of rats. TQ was given by oral gavage (10 mg/kg per day) for two weeks. HT2AR and pro-caspase-3 levels were measured by the enzyme-linked immunosorbent assay (ELISA). Morphological changes in the liver were identified by hematoxylin and eosin staining. Results: In the P.gingivalis-LPS injected group, the hepatic HT2AR levels increased while pro-caspase-3 levels decreased, disturbing the liver morphological structure. TQ administration increased pro-caspase-3 levels and improved morphological structure despite increased HT2AR levels. Conclusion: Our results suggest that oral application of P. gingivalis-LPS decreased pro-caspase 3, an inactive form of caspase-3 by HT2AR, probably due to increased cleaved caspase-3 levels, and TQ administration increased pro-caspase-3 levels, leading to improved liver morphological structure. Therephore, our study indicated that oral pathogen P. gingivalis might impact the liver HT2AR-mediated, and TQ may have a beneficial effect.

Keywords

Ethical Statement

30.01.2025-2025/2-9

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other), Veterinary Biochemistry

Journal Section

Research Article

Authors

Ebru Afşar ^*
0000-0002-7817-855X
Türkiye

Işıl Eranıl
0000-0001-9557-8523
Türkiye

Erkan Özkan
0000-0002-5251-2302
Türkiye

Erdem Aslan
0000-0002-4992-5915
Türkiye

Kadirhan Doğan
0000-0002-0249-1435
Türkiye

Mehmet Öz
0000-0003-4167-2623
Türkiye

Publication Date

August 31, 2025

Submission Date

March 11, 2025

Acceptance Date

June 30, 2025

Published in Issue

Year 2025 Volume: 10 Number: 2

DOI

https://doi.org/10.31797/vetbio.1655445

IZ

https://izlik.org/JA52HW43NE

Cite

RIS / Bibtex

APA

Afşar, E., Eranıl, I., Özkan, E., Aslan, E., Doğan, K., & Öz, M. (2025). Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes. Journal of Advances in VetBio Science and Techniques, 10(2), 108-114. https://doi.org/10.31797/vetbio.1655445

AMA

1.Afşar E, Eranıl I, Özkan E, Aslan E, Doğan K, Öz M. Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes. J Ad VetBio Sci Tech. 2025;10(2):108-114. doi:10.31797/vetbio.1655445

Chicago

Afşar, Ebru, Işıl Eranıl, Erkan Özkan, Erdem Aslan, Kadirhan Doğan, and Mehmet Öz. 2025. “Investigation of the Changes in Hepatic 5-Hydroxytryptamine 2A Receptor Levels Caused by Lipopolysaccharide of Porphyromonas Gingivalis and the Effect of Thymoquinone on These Changes”. Journal of Advances in VetBio Science and Techniques 10 (2): 108-14. https://doi.org/10.31797/vetbio.1655445.

EndNote

Afşar E, Eranıl I, Özkan E, Aslan E, Doğan K, Öz M (August 1, 2025) Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes. Journal of Advances in VetBio Science and Techniques 10 2 108–114.

IEEE

[1]E. Afşar, I. Eranıl, E. Özkan, E. Aslan, K. Doğan, and M. Öz, “Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes”., J Ad VetBio Sci Tech, vol. 10, no. 2, pp. 108–114, Aug. 2025, doi: 10.31797/vetbio.1655445.

ISNAD

Afşar, Ebru - Eranıl, Işıl - Özkan, Erkan - Aslan, Erdem - Doğan, Kadirhan - Öz, Mehmet. “Investigation of the Changes in Hepatic 5-Hydroxytryptamine 2A Receptor Levels Caused by Lipopolysaccharide of Porphyromonas Gingivalis and the Effect of Thymoquinone on These Changes”. Journal of Advances in VetBio Science and Techniques 10/2 (August 1, 2025): 108-114. https://doi.org/10.31797/vetbio.1655445.

JAMA

1.Afşar E, Eranıl I, Özkan E, Aslan E, Doğan K, Öz M. Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes. J Ad VetBio Sci Tech. 2025;10:108–114.

MLA

Afşar, Ebru, et al. “Investigation of the Changes in Hepatic 5-Hydroxytryptamine 2A Receptor Levels Caused by Lipopolysaccharide of Porphyromonas Gingivalis and the Effect of Thymoquinone on These Changes”. Journal of Advances in VetBio Science and Techniques, vol. 10, no. 2, Aug. 2025, pp. 108-14, doi:10.31797/vetbio.1655445.

Vancouver

1.Ebru Afşar, Işıl Eranıl, Erkan Özkan, Erdem Aslan, Kadirhan Doğan, Mehmet Öz. Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes. J Ad VetBio Sci Tech. 2025 Aug. 1;10(2):108-14. doi:10.31797/vetbio.1655445