Investigation of Cryptosporidium spp. in water sources shared by humans and animals in the Kars region, Türkiye
Year 2025,
Volume: 10 Issue: 3, 175 - 179, 31.12.2025
Neslihan Ölmez
,
Nilgün Aydın
,
Barış Sarı
,
Mükremin Özkan Arslan
Abstract
Cryptosporidium is protozoan infects a wide range of animals as well as humans and one of waterborne parasites considered a major public health problem in developing and developed countries. In this study, it was aimed to investigate Cryptosporidium spp. oocysts in the water sources commonly used by humans and animals in Kars region, Türkiye. In this study, 38 samples were collected from streams and pond at all district in Kars Region. All samples were first passed through a vacuum filtration device and then the sediments were stained with carbol fuchsin and the modified acid fast (mAF) method. As a result, Cryptosporidium oocysts with zoonotic potential were found in 13.2% (5/38). According to the results obtained, the detection of this parasite, which is commonly seen in animals in the Kars region, in water has revealed that it poses a significant risk to public health. Considering these data, preventing waste from mixing into water in water sources that are used jointly by humans and animals is extremely important in preventing the spread of this protozoan.
Ethical Statement
KAU-HADYEK/2024-005
References
-
Akkaş, Ö., Gürbüz, E., Aydemir, S., Şahin, M., & Ekici, A. (2023). Farklı kaynak sularından alınan örneklerde Giardia spp., Cryptosporidium spp. ve Cyclospora cayetanensis’ in araştırılması Iğdır, Türkiye. Türkiye Parazitoloji Dergisi, 47(2):71-77. https://doi.org/10.4274/tpd.galenos.2023.74936
-
Arslan, M., Sarı, B., Kara, M., Tașçi, G., Ekinci, A., & Gündüz, N. (2012). Research on the prevalence of Eimeria and Cryptosporidium species in cows in periparturient period in Kars region. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(Supplement A), A65–A70. https://doi.org/10.9775/kvfd.2011.5993
-
Arslan, M. Ö., Gıcık, Y., Erdoğan, H. M., & Sarı, B. (2001). Prevalence of Cryptosporidium spp. oocysts in diarrhoeic calves in Kars Province, Turkey. Turkish Journal of Veterinary & Animal Sciences, 25(2), 161-164.
-
Ayan, A., Celik, B. A., Celik, O. Y., Akyildiz, G., Kilinc, O. O., Ayan, O. O., Ertas Oguz, F., Goz, Y., Nazmi Yuksek, N., Yilmaz, A. B., Myrzhiyeva, A., Akhmetzhanova, A., and Uslu, U. (2024). First report of zoonotic Cryptosporidium parvum subtype IIaA15G2R1 in dogs in Türkiye. Pakistan Veterinary Journal, 44(4), 1263-1268. https://doi.org/10.29261/pakvetj/2024.274
-
Aydemir, S., Barlık, F., Ekici, A., Barlık, D. H., Alkan, S., Gürbüz, E., & Yılmaz, H. (2024). Molecular characterization of Giardia intestinalis and Cryptosporidium spp. detected in Humans in Ağrı, Türkiye. Iranian Journal of Parasitology, 19(1), 9. https://doi.org/10.18502/ijpa.v19i1.15188
-
Ayed, L. B., Ahmed, S. A. A., Boughattas, S., & Karanis, P. (2024). Waterborne Cryptosporidium species and Giardia duodenalis in resources of MENA: A systematic review and meta-analysis. Journal of Water and Health, 22(8), 1491-1515. https://doi.org/10.2166/wh.2024.107
-
Baldursson, S., & Karanis, P. (2011). Waterborne transmission of protozoan parasites: Review of worldwide outbreaks–an update 2004–2010. Water Research, 45(20), 6603-6614. https://doi.org/10.1016/j.watres.2011.10.013
-
Bourli, P., Eslahi, A. V., Tzoraki, O., & Karanis, P. (2023). Waterborne transmission of protozoan parasites: a review of worldwide outbreaks–an update 2017–2022. Journal of Water and Health, 21(10), 1421-1447. https://doi.org/10.2166/wh.2023.094
-
Ceber, K., Aslan, G., Otağ, F., Delialioğlu, N., Öztürk, C., Babür, C., & Emekdaş, G. (2005). Investigation of Cryptosporidium spp. oocysts in tap water, well water, sewage water and sea water in Mersin, Turkey. Türkiye Parazitoloji Dergisi, 29(4), 224-228.
-
Cheng, J., Karambelkar, B., Xie, Y., Wickham, H., Russell, K., & Johnson, K. (2022). Leaflet: Create interactive web maps with the javascript ‘leaflet’library. 2019. URL https://CRAN. R-project. org/package= leaflet. R package version, 1(0), 102.
-
Chuah, C. J., Mukhaidin, N., Choy, S. H., Smith, G. J., Mendenhall, I. H., Lim, Y. A., & Ziegler, A. D. (2016). Prevalence of Cryptosporidium and Giardia in the water resources of the Kuang River catchment, Northern Thailand. Science of the Total Environment, 562, 701-713. https://doi.org/10.1016/j.scitotenv.2016.03.247
-
Çelik, Ö. Y., Şahin, T., Çelik, B. A., Kılınç, Ö. O., Ayan, A., Akyıldız, G., Ayan Ö. O., Göz, Y., İrak, K., Görmez, G. (2023). Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Türkiye. Acta Veterinaria Brno, 92(3), 243-250. https://doi.org/10.2754/avb202392030243
-
Çiçek, M., Körkoca, H., & Akkaş, Ö. (2011). Van ili içme sularının Cryptosporidium spp. ookistleri yönünden incelenmesi. Türk Hijyen ve Deneysel Biyoloji Dergisi, 68(3), 122-126. https://doi.org/10.5505/TurkHijyen.2011.26214
-
Çuhadar, V., Şengül, M., & Mete, E. (2023). Mardin’deki su kaynaklarında Cryptosporidium parvum’un araştırılması. Türk Mikrobiyoloji Cemiyeti Dergisi, 53(3), 156-162. https://doi.org/10.54453/TMCD.2023.94546
-
Daraei, H., Oliveri Conti, G., Sahlabadi, F., Thai, V. N., Gholipour, S., Turki, H., Fakhri, Y., Ferrante, M., Moradi, A., & Mousavi Khaneghah, A (2021). Prevalence of Cryptosporidium spp. in water: a global systematic review and meta-analysis. Environmental Science and Pollution Research, 28, 9498-9507. https://doi.org/10.1007/s11356-020-11261-6
-
Dixon, B. R. (2016). Parasitic illnesses associated with the consumption of fresh produce—an emerging issue in developed countries. Current Opinion in Food Science, 8, 104-109. https://doi.org/10.1016/j.cofs.2016.04.009
-
Dong, S., Yang, Y., Wang, Y., Yang, D., Yang, Y., Shi, Y., Li, C., Li, L., Chen, Y., Jiang, Q., & Zhou, Y. (2020). Prevalence of Cryptosporidium infection in the global population: a systematic review and meta-analysis. Acta Parasitologica, 65, 882-889. https://doi.org/10.2478/s11686-020-00230-1
-
Efstratiou, A., Ongerth, J. E., & Karanis, P. (2017). Waterborne transmission of protozoan parasites: review of worldwide outbreaks-an update 2011–2016. Water Research, 114, 14-22. https://doi.org/0.1016/j.watres.2017.01.036
-
Esri, M. E. G. (2023). World Imagery Map. https://www.arcgis.com/home/item.html?id=10df2279f9684e4a9f6a7f08febac2a9
-
Fayer, R. (2004). Cryptosporidium: A water-borne zoonotic parasite. Veterinary Parasitology, 126(1-2), 37-56. https://doi.org/10.1016/j.vetpar.2004.09.004
-
Gallas-Lindemann, C., Sotiriadou, I., Plutzer, J., & Karanis, P. (2013). Prevalence and distribution of Cryptosporidium and Giardia in wastewater and the surface, drinking and ground waters in the Lower Rhine, Germany. Epidemiology & Infection, 141(1), 9-21. https://doi.org/10.1017/S0950268812002026
-
Gündüz, N., & Arslan, M. Ö. (2017). Determining the prevalence of Cryptosporidium infections with acid fast staining and ELISA in calves at the Kars province of Turkey. Türkiye Parazitoloji Dergisi, 41(1), 5. https://doi.org/10.5152/tpd.2017.4833
-
Hamilton, K. A., Waso, M., Reyneke, B., Saeidi, N., Levine, A., Lalancette, C., Besner, M.-C., Khan, W., & Ahmed, W. (2018). Cryptosporidium and Giardia in wastewater and surface water environments. Journal of Environmental Quality, 47(5), 1006-1023. https://doi.org/10.2134/jeq2018.04.0132
-
Kaya, Y., Karaman, Ü., & Yolalan, G. (2021). Ordu ilinde kaynak sularının parazit varlığı açısından değerlendirilmesi. ODÜ Tıp Dergisi, 8(1), 14-18.
-
Liu, J., Platts-Mills, J. A., Juma, J., Kabir, F., Nkeze, J., Okoi, C., Operario, D. J., Uddin, J., Ahmed, S., Alonso, P. L., Antonio, M., Becker, S. M., Blackwelder, W. C., Breiman, R. F., Faruque, A. S. G., Fields, B., Gratz, J., Haque, R., Hossain, A., Hossain, M. J., ... Houpt, E. R. (2016). Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the GEMS case-control study. The Lancet, 388(10051), 1291-1301. https://doi.org/10.1016/S0140-6736(16)31529-X
-
Mahmoudi, M. R., Nazemalhosseini-Mojarad, E., Kazemi, B., Haghighi, A., Mirzaei, A., Mohammadiha, A., Jahantab, S., Xiao, L., & Karanis, P. (2015). Cryptosporidium genotypes and subtypes distribution in river water in Iran. Journal of Water and Health, 13(2), 600-606. https://doi.org/10.2166/wh.2014.234
-
Özçelik, S., Malatyalı, E., Alim, A., & Değerli, S. (2015). The investigation of Cryptosporidium spp. in water samples by PCR. Cumhuriyet Tıp Dergisi, 37(3), 182-187. https://doi.org/10.7197/cmj.v37i3.5000120833
-
Özkan, F., & İça, A. (2024). Molecular diagnosis and typing of Cryptosporidium spp. species in human stools with diarrhea. The Journal of Molecular Diagnostics, 48(3), 184-190. https://doi.org/10.4274/tpd.galenos.2024.60392
-
Sağlam, T., Düşen, S., Karaman, Ü., & Mete, E. (2022). Denizli il merkezindeki içme sularında ve çevresel sularda protozoon parazitlerinin varlığı. Türkiye Parazitoloji Dergisi, 46(3), 134–139. https://doi.org/10.4274/tpd.galenos.2022.47966
-
Sahin, O. F., Erol, U., Urhan, O. F., Sakar, H. F., & Altay, K. (2025). Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in water buffaloes (Bubalis bubalis) from Türkiye. Acta Parasitologica, 70(1), 6. https://doi.org/10.1007/s11686-024-00975-z
-
Sari, B., Arslan, M. Ö., Gicik, Y., Kara, M., & Taşçi, G. T. (2009). The prevalence of Cryptosporidium species in diarrhoeic lambs in Kars province and potential risk factors. Tropical Animal Health and Production, 41(5), 819-826. https://doi.org/10.1007/s11250-008-9260-0
-
Sayın İpek, D. N., & Sarı, B. (2024). Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in lambs and calves in Southeastern Anatolia, Turkey. Egyptian Journal of Veterinary Sciences, 55(2), 599-606. https://doi.org/10.21608/ejvs.2023.239745.1631
-
Shirley, D. A., Moonah, S. N., & Kotloff, K. L. (2012). Burden of disease from cryptosporidiosis. Current Opinion in Infectious Diseases, 25(5), 555-563. https://doi.org/10.1097/QCO.0b013e328357e569
-
Vanathy, K., Parija, S. C., Mandal, J., Hamide, A., & Krishnamurthy, S. (2017). Cryptosporidiosis: A mini review. Tropical Parasitology, 7(2), 72-80. https://doi.org/10.4103/tp.TP_25_17
-
Yalçın, S., & Doğan, N. Y. (2019). Erzincan ili farklı su kaynaklarından Cryptosporidium spp’nin moleküler yöntemlerle tespit edilmesi. Erzincan University Journal of Science and Technology, 12(1), 1-13. https://doi.org/10.18185/erzifbed.342568