Oxidative stress in Neospora-positive dairy cows with embryonic losses

Gaye Bulut; Caner Ozturk; Neşe Hayat Aksoy

doi:10.31797/vetbio.1780081

Oxidative stress in Neospora-positive dairy cows with embryonic losses

Abstract

This study was conducted to evaluate the oxidative status of Holstein-Friesian cows that experienced embryonic loss and were either seropositive or seronegative for Neospora caninum. Mean total antioxidant status (TAS) was similar between groups, ranging from 952.6 ± 499.0 to 1110.3 ± 260.0 µmol/L in Neospora- positive (NP) cows and from 1233.1 ± 372.4 to 1739.7 ± 599.1 µmol/L in Neospora- negative (NN) cows (p > 0.05). In contrast, total oxidant status (TOS) and oxidative stress index (OSI) increased significantly with advancing lactation in NP cows. TOS rose from 52.4 ± 10.3 µmol/L in the 2nd lactation to 114.8 ± 51.0 and 143.3 ± 29.5 µmol/L in the 4th and 5th lactations (p <0.05). Similarly, OSI increased from 8.09 ± 7.56 in the 2nd lactation to 12.91 ± 3.83 and 13.78 ± 5.43 in the 4th and 5th lactations (p <0.05). NN cows maintained lower oxidative stress, with TOS between 25.3 ± 5.4 and 51.4 ± 14.1 µmol/L and OSI between 1.57 ± 0.48 and 4.39 ± 1.62. The lowest OSI was observed in NN cows in the 2nd lactation (1.57 ± 0.48; p <0.05). These findings suggest that N. caninum infection exacerbates oxidative imbalance, particularly in later lactations, increasing susceptibility to reproductive failure. Systemic biomarkers may underestimate this effect; therefore, further tissue-level studies are needed to clarify the pathogen’s contribution to embryonic loss.

Keywords

Supporting Institution

The authors declared that this study has received no financial support.

Ethical Statement

This study was approved by the Local Animal Experiment Ethics Committee of Aksaray University (Approval No: 2024/5-30, Date: June 2, 2024). The study was conducted in accordance with national and institutional guidelines for the care and use of animals.

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Details

Primary Language

English

Subjects

Veterinary Obstetrics and Gynecology

Journal Section

Research Article

Authors

Gaye Bulut ^*
0000-0003-4500-1958
Türkiye

Caner Ozturk
0000-0003-0566-0684
Türkiye

Neşe Hayat Aksoy
0000-0001-9039-555X
Türkiye

Publication Date

December 31, 2025

Submission Date

September 8, 2025

Acceptance Date

November 21, 2025

Published in Issue

Year 2025 Volume: 10 Number: 3

DOI

https://doi.org/10.31797/vetbio.1780081

IZ

https://izlik.org/JA44CS28UT

Cite

RIS / Bibtex

APA

Bulut, G., Ozturk, C., & Aksoy, N. H. (2025). Oxidative stress in Neospora-positive dairy cows with embryonic losses. Journal of Advances in VetBio Science and Techniques, 10(3), 188-193. https://doi.org/10.31797/vetbio.1780081

AMA

1.Bulut G, Ozturk C, Aksoy NH. Oxidative stress in Neospora-positive dairy cows with embryonic losses. J Ad VetBio Sci Tech. 2025;10(3):188-193. doi:10.31797/vetbio.1780081

Chicago

Bulut, Gaye, Caner Ozturk, and Neşe Hayat Aksoy. 2025. “Oxidative Stress in Neospora-Positive Dairy Cows With Embryonic Losses”. Journal of Advances in VetBio Science and Techniques 10 (3): 188-93. https://doi.org/10.31797/vetbio.1780081.

EndNote

Bulut G, Ozturk C, Aksoy NH (December 1, 2025) Oxidative stress in Neospora-positive dairy cows with embryonic losses. Journal of Advances in VetBio Science and Techniques 10 3 188–193.

IEEE

[1]G. Bulut, C. Ozturk, and N. H. Aksoy, “Oxidative stress in Neospora-positive dairy cows with embryonic losses”, J Ad VetBio Sci Tech, vol. 10, no. 3, pp. 188–193, Dec. 2025, doi: 10.31797/vetbio.1780081.

ISNAD

Bulut, Gaye - Ozturk, Caner - Aksoy, Neşe Hayat. “Oxidative Stress in Neospora-Positive Dairy Cows With Embryonic Losses”. Journal of Advances in VetBio Science and Techniques 10/3 (December 1, 2025): 188-193. https://doi.org/10.31797/vetbio.1780081.

JAMA

1.Bulut G, Ozturk C, Aksoy NH. Oxidative stress in Neospora-positive dairy cows with embryonic losses. J Ad VetBio Sci Tech. 2025;10:188–193.

MLA

Bulut, Gaye, et al. “Oxidative Stress in Neospora-Positive Dairy Cows With Embryonic Losses”. Journal of Advances in VetBio Science and Techniques, vol. 10, no. 3, Dec. 2025, pp. 188-93, doi:10.31797/vetbio.1780081.

Vancouver

1.Gaye Bulut, Caner Ozturk, Neşe Hayat Aksoy. Oxidative stress in Neospora-positive dairy cows with embryonic losses. J Ad VetBio Sci Tech. 2025 Dec. 1;10(3):188-93. doi:10.31797/vetbio.1780081