Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain

Ali Şenol; Taha Altuğ; Ali Karapinar; Özge Avşar

doi:10.31797/vetbio.1867408

Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain

Abstract

This study aimed to evaluate the effects of high-fructose corn syrup (HFCS) and citric acid (CA) on the oxidative and antioxidant status of brain tissues in broiler chickens. The one-day-old Ross 308 chicks used in this study were randomly allocated into four experimental groups: control, 30 mg/kg CA, 50 mg/kg HFCS, and 30 mg/kg CA + 50 mg/kg HFCS. At the end of the experimental period, nine chicks from each group were slaughtered, and brain tissues were collected. Cerebrum and cerebellum regions were separately analyzed, and oxidative stress markers were quantified. Results demonstrated that total oxidant status (TOS), oxidative stress index (OSI) malondialdehyde (MDA), and nitric oxide (NO) levels were highest in both brain regions of the HFCS group compared to the other groups. In the cerebrum, NO and OSI elevations were statistically significant, whereas in the cerebellum, TOS, OSI, and MDA values were significantly increased in the HFCS group. Total antioxidant status (TAS), indicative of antioxidant defense, was highest in the CA group across both brain regions; cerebrum TAS levels were significantly higher than those in the HFCS group, while in the cerebellum, this increase was statistically significant compared to all groups. In conclusion, HFCS exposure induced oxidative and nitrosative stress in the cerebrum and cerebellum of broiler chickens, whereas citric acid supplementation significantly reduced oxidative damage in both regions.

Keywords

Supporting Institution

No private funding was received for this study

Ethical Statement

This study was conducted following the approval of the Local Animal Experiments Ethics Committee of Kırıkkale University (Date: 24.11.2022, No: 2022-06), Kırıkkale, Türkiye.

Thanks

This study was conducted using biological material (broiler brain tissue) obtained from the study titled "Effect of High Fructose Corn Syrup and Citric Acid in Broiler Diets on Performance, Gut pH, Immunity, Antioxidant Status, and Some Blood Parameters." The material has not been used in any other research or published. We would like to thank Assist. Prof. Dr. Gökhan ŞEN and Prof. Dr. Miyase ÇINAR for their support in providing the material and conducting the study.

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Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Ali Şenol ^*
0000-0003-4080-7776
Türkiye

Taha Altuğ
0000-0002-7181-4074
Türkiye

Ali Karapinar
0000-0002-6589-1807
Türkiye

Özge Avşar
0000-0002-2600-4371
Türkiye

Publication Date

May 6, 2026

Submission Date

January 20, 2026

Acceptance Date

March 23, 2026

Published in Issue

Year 2026 Number: 2

DOI

https://doi.org/10.31797/vetbio.1867408

IZ

https://izlik.org/JA73GS85TH

Cite

RIS / Bibtex

APA

Şenol, A., Altuğ, T., Karapinar, A., & Avşar, Ö. (2026). Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain. Journal of Advances in VetBio Science and Techniques, 2, 85-91. https://doi.org/10.31797/vetbio.1867408

AMA

1.Şenol A, Altuğ T, Karapinar A, Avşar Ö. Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain. J Ad VetBio Sci Tech. 2026;(2):85-91. doi:10.31797/vetbio.1867408

Chicago

Şenol, Ali, Taha Altuğ, Ali Karapinar, and Özge Avşar. 2026. “Protective Effects of Citric Acid Against High-Fructose Corn Syrup-Induced Oxidative Stress in Broiler Brain”. Journal of Advances in VetBio Science and Techniques, nos. 2: 85-91. https://doi.org/10.31797/vetbio.1867408.

EndNote

Şenol A, Altuğ T, Karapinar A, Avşar Ö (May 1, 2026) Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain. Journal of Advances in VetBio Science and Techniques 2 85–91.

IEEE

[1]A. Şenol, T. Altuğ, A. Karapinar, and Ö. Avşar, “Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain”, J Ad VetBio Sci Tech, no. 2, pp. 85–91, May 2026, doi: 10.31797/vetbio.1867408.

ISNAD

Şenol, Ali - Altuğ, Taha - Karapinar, Ali - Avşar, Özge. “Protective Effects of Citric Acid Against High-Fructose Corn Syrup-Induced Oxidative Stress in Broiler Brain”. Journal of Advances in VetBio Science and Techniques. 2 (May 1, 2026): 85-91. https://doi.org/10.31797/vetbio.1867408.

JAMA

1.Şenol A, Altuğ T, Karapinar A, Avşar Ö. Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain. J Ad VetBio Sci Tech. 2026;:85–91.

MLA

Şenol, Ali, et al. “Protective Effects of Citric Acid Against High-Fructose Corn Syrup-Induced Oxidative Stress in Broiler Brain”. Journal of Advances in VetBio Science and Techniques, no. 2, May 2026, pp. 85-91, doi:10.31797/vetbio.1867408.

Vancouver

1.Ali Şenol, Taha Altuğ, Ali Karapinar, Özge Avşar. Protective effects of citric acid against high-fructose corn syrup-induced oxidative stress in broiler brain. J Ad VetBio Sci Tech. 2026 May 1;(2):85-91. doi:10.31797/vetbio.1867408