Investigation of the Response of Different Breeds to the Superovulation Treatment

Abstract

This study was carried out to compare three different breeds (Simmental, Holstein and Brown Swiss) for the response to the superovulation program and embryo quality. Cows from each breed which have given at least one birth and which are at the same age and conditions were used. Regardless of the period of the oestrus cycle, intravaginal CIDR (Pfizer Inc., New York, USA) implant including 1.38 g progesteron was applied to each cow and kept 9 days long. A total of 400 mg i.m.  FSH (PLUSET/Laboratorios Calier) was injected twice a day during 4 days from 7th day after implantation of CIDR to cows. All animals were injected 150 µg d-choprostenol (Dalmazin/Vetaş) together with 5th FSH injection, and right after 6th FSH injection CIDR was removed. After the last FSH injection, in the 12th and 24th hours from the beginning of oestrus, cows were inseminated. Seven days after first insemination, embryos were collected via non-surgical uterine flushing. According to the results, superovulation response was better in Simmental cows than the others. The best response for superovulation in FSH hormone was also observed in Simmental breed cows.

Keywords

• Superovulation,Embryo,Simmental,Holstein,Brown Swiss

Süperovulasyon Uygulamasına Farklı Irkların Verdikleri Cevapların Araştırılması

Abstract

Bu çalışma, süperovulasyon programı uygulanan 3 farklı sığır ırkının (Simental, Siyah-Alaca ve Esmer) süperovulasyon uygulamasına verdikleri cevapların ve elde edilen embriyo kalitelerinin karşılaştırılması amacıyla yapıldı. Çalışmada her ırktan en az bir doğum yapmış aynı yaş ve kondisyonlara sahip inekler kullanıldı.      Her ineğe östrus siklusunun herhangi bir döneminde 1.38 g progesteron içeren intravaginal CIDR (Pfizer Inc., New York, USA) 9 gün  süreyle  uygulandı. Her ineğe progesteron uygulamasının 7. gününden başlamak üzere günde 2 defa, 4 gün boyunca toplam 400 mg i.m. FSH (PLUSET/Laboratorios Calier) uygulaması yapıldı. Bütün hayvanlara 5. FSH uygulaması ile birlikte 150 µg. d-choprostenol (Dalmazin/Vetaş) yapıldı ve 6. FSH uygulaması ile birlikte CIDR uzaklaştırıldı. Son FSH uygulamasının ardından östrus belirtilerinin 12. ve 24. saatlerinde suni tohumlama yapıldı. İlk suni tohumlamadan 7 (yedi) gün sonra non-surgical yöntemle uterus yıkaması yapılarak embriyolar toplandı. Elde edilen sonuçlara göre Simental ırkı ineklerin süperovulasyon uygulamasına olan cevabının diğer ırklardan daha iyi olduğu gözlendi

Keywords

• Süperovulasyon,Embriyo,Simental,Siyah Alaca,Esmer

References

1. Hızlı H, Ayaşan T, Kılıçalp N, Kara U, Karakozak E, Özcan BD, Gök K, Çamlıdağ A, Çoban S, Mutlu H, Seğmenoğlu MS (2012): Verici inek ve düvelerde tekrarlanan süperovulasyonların embriyo kalitesi üzerine etkisi. Van Vet J, 23(1): 11-14.
2. Akyol N, Kızıl SH, Satılmış M, Karaşahin T, Erat S (2014): The results of consecutive superovulations in cows by induction with various exogenous progesterone routes. Turk J Vet Anim Sci 38: 157-160.
3. Barati F, Niasari_Naslaji A, Bolourchi M., Sarhaddi F, Razavi K, Naghzali E, Thatcher WW (2006): Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology 66: 1149-1155.
4. Baruselli PS, Sa Filho MF, Martins CM, Nasser LF, Nogueira MF, Barros CM, Bo GA (2006): Superovulation and embryo transfer in Bos indicus cattle. Theriogenology 65: 77-88.
5. Bülbül B, Kırbaş M, Köse M, Dursun Ş (2010): Investigation of superovulation response in brown swiss cows after synchronization using progesterone and oestradiol valerate. Kafkas Univ Vet Fak Derg 16(3): 463-468.
6. Demczuk E, Kozıckı LE, Pontellı ES, Salles JO (1998): Embryo transfer in Simmental cows in northwest Paraná and South of Mato Grosso do Sul. Braz J Vet Res Anim Sci 35(4): 174-177.
7. Detterer J, Schmidt T, Harlizius B (1997): Factors influencing the variability in superovulation results in German Holstein cattle. Theriogenology 47(1): 169, Proceedings Annual Conference of the International Embryo Transfer Society.
8. Hasler JF, Brooke GP, McCauley AD (1981): The relationship between age and response to superovulation in holstein cows and heifers. Theriogenology, 15(1): 109.

9. Hasler JF (1992): Current status and potential of embryo transfer and reproductive technology in dairy cattle. J Dairy Sci 75: 2857-2879.
10. Hasler JF (2010): Bovine embryo transfer: are efficiencies improving? Applied Reproductive Strategies Conference Proceedings August 5th & 6th, Nashville, TN. 265-282.
11. Hussein MM, Abdel Aziz RL, Abdel-Wahab A, El-Said H (2014): Preliminary study of factors affecting the superovulatory response of high producing dairy cows superstimulated regardless of the stage of estrous cycle in Egypt. Beni-Seuf Univ J Appl Sci 3: 286-292.
12. Kafi M, McGowan MR (1997): Factors associated with variation in the superovulatory response of cattle. Anim Reprod Sci 48:137-157.
13. Kelly P, Duffy P, Roche JF, Boland MP (1997): Superovulation in cattle: effect. of FSH type and method of administration on follicular growth, ovulatory response and endocrine patterns. Anim Reprod Sci 46:1-14.
14. Mapletoft RJ, Steward KB, Adams GP (2002): Recent advances in the superovulation in cattle. Reprod Nutr Dev 42: 601-611.
15. Nascımento AB, Seneda MM, da Sılveıra EC, Pansard H (2004): Gonadotrophin association in super-ovulation of embryo donor cows. Cienc anim bras 14(2): 89-94.
16. Nilchuen P, Rattanatabtimtong S, Chomchai S (2011): Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle. Songklanakarin J Sci Technol 33(6): 679-683.
17. Quaresma MA, Costa LL, Silva JR (2003): Superovulation of Mertolenga cows with two FSH preparations (FSH-P and FOLLTROPIN). Rev Port Cien Vet 98(546): 81-84.
18. Silva JCC, Alvarez RH, Zanenga CA, Pereira GT (2009): Factors affecting embryo production in superovulated Nelore cattle. Anim Repro 6:440-445.
19. Stroud B, Hasler JF (2006): Dissecting why superovulation and embryo transfer usually work on some farms but not on others. Theriogenology 65: 65-76.
20. Vieira LM, Rodrigues CA, Mendanha MF, Sá Filho MF, Sales JNS, Souza AH, Santos JEP, Baruselli PS (2014): Donor category and seasonal climate associated with embryo production and survival in multiple ovulation and embryo transfer programs in Holstein cattle. Theriogenology 82: 204-212.
21. Wright JM (1998): Photographic illustrations of embryo developmental stage and quality codes. In, Stringfellow DA, Seidel SM (Eds): Manual of the International Embryo Transfer Society. 3rd ed., pp. 167-170, Savoy, Illinosis,