Yavru Gökkuşağı alabalıkları (Oncorhynchus mykiss, W.)’ndan Lactococcus garvieae’nin İzolasyonu ve Plazmit Profilleri Üzerine Bir Çalışma

Öz

Bu çalışmada Fethiye bölgesindeki bazı gökkuşağı alabalığı (Oncorhynchus mykiss) işletmelerindeki hasta yavru balıklardan Lactococcus garvieae suşları izole ve identifiye edilmiştir. Hasta balıklarda durgunluk, iştahsızlık, gözlerde ekzoftalmi ve hemoraji, abdominal kısımda şişkinlik gözlenmiştir. İç bulgu olarak karaciğerde hemoraji, dalakta büyüme (splenomegali) ve karın boşluğunda asidik sıvı birikimi tespit edilmiştir. Lactococcus garvieae suşları ampisilin, eritromisin, kloramfenikol, sülfametoksazol, oksitetrasiklin, tetrasiklin ve trimetoprim’e duyarlılık, basitrasin, flumekuin, furazolidon, kanamisin, nalidiksik asit, oksalinik asit ve streptomisin’e direnç göstermiştir. Plazmit analiz sonuçlarına göre, suşların farklı boyutlarda plazmitleri içerdikleri bulunmuştur.

Anahtar Kelimeler

• Gökkuşağı alabalığı
• Lactococcus garvieae
• antimikrobiyal
• plazmit

A Study on Plasmid Profiles and Isolation of Lactococcus garvieae from the Young Rainbow Trout (Oncorhynchus mykiss, W.)

Abstract

In this study, Lactococcus garvieae strains were isolated and identified from sick young fish from some rainbow trout (Oncorhynchus mykiss) farms in Fethiye region. Lethargy, anorexia, exophthalmi with hemorrhage and abdominal dropsy in the sick fish were observed. Hemorrhage in the liver, enlargement of the spleen (splenomegaly) and ascites as internal signs were determined. The strains showed sensitivity to ampicillin, erythromycine, chloramphenicol, sulfamethoxazole, oxytetracycline, tetracycline and trimethoprim and resistant to basitrasin, flumequine, furazolidone, kanamycin, nalidixic acid, oxalinic acid and streptomycine. It was found that the strains harboured plasmids with different sizes according to the results of plasmid analysis results.

Keywords

• Rainbow trout
• Lactococcus garvieae
• antimicrobial
• plasmid

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