Effect of additives on short-term storage of ram semen

Abstract

The aim of this study was to investigate the effects of short-term stored ram semen (24-hour intervals) on spermatological parameters up to 72 hours. In the study, % motility, viability and acrosome integrity were evaluated. In thıs study four ejaculates from each ram (four merino rams) were used. The ejaculates were divided into seven equal-sized pieces after pooling. The study groups were formed as resveratrol (2 and 4 mM), trolox (2 and 4 mM), BSA (3 and 6 mg/ml) and control added to tris-based diluent. Diluted samples were stored at 4 ° C until 72 hours. Spermatozoa motility, viability and acrosome integrity were evaluated at 0, 24, 48 and 72 hours. Motility examination was evaluated with phase contrast microscope at 400X magnification, vitality and acrosome integrity were evaluated with fluorescent microscope. At the 72nd hour of the study, the BSA (3 and 6mM) groups were assessed for motility (53.75 ± 2.50%; 55.00 ± 4.08%) and acrosome integrity (53.70 ± 3.39%; 57.10 ± 4.68%) in the control group (43.75 ± 2.50%; 46.53 ± 3.58%) and statistical difference (p <0.05). In the Trolox (4mM) group (67.22 ± 3.71%), the highest viability was achieved at 72 hours compared to all groups, and a statistical difference (p <0.05) was determined with the control group (46.53 ± 3.53). As a result of the study, it was determined that BSA and trolox added to ram semen extender were beneficial in the short-term storage of ram semen and had a protective effect on spermatological parameters.

Keywords

• BSA
• short term storage
• trehalose
• trolox

Koç spermasının kısa süreli saklanmasında katkı maddelerinin etkisi

Abstract

Bu çalışmanın amacı, kısa süreli saklanan koç spermasının (24 saatlik aralıklarla) 72 saate kadar spermatolojik parametreler üzerine etkilerini araştırmaktı. Çalışmada % motilite, canlılık ve akrozom bütünlüğü verileri değerlendirildi. Çalışmada her koçtan (4 baş Merinos koçu) alınan dört ejakülat kullanıldı. Ejakülatlar pooling yapıldıktan sonra eşit hacimli yedi parçaya bölündü. Çalışma grupları, tris bazlı sulandırıcıya eklenen resveratrol (2 ve 4 mM), troloks (2 ve 4 mM), BSA (3 ve 6 mg/ml) ve kontrol olmak üzere oluşturuldu. Sulandırılan örnekler 72. saate kadar 4°C de muhafaza edildi. Spermatozoa motilitesi, canlılık ve akrozom bütünlüğü 0, 24, 48 ve 72. saatte değerlendirildi. Motilite muayenesi faz kontrast ataçmanlı mikroskopta 400X lük büyütmede, canlılık ve akrozom bütünlüğü ise floresan mikroskop ile değerlendirildi. Çalışmanın 72. saatinde, BSA (3 ve 6mM) grupları motilite (53.75±2.50%; 55.00±4.08%) ve akrozom bütünlüğü (53.70±3.39%; 57.10±4.68%) değerlendirmelerinde kontrol grubu (43.75±2.50%; 46.53±3.58) ile istatistiksel farklılık (p<0.05) gösterdi. Troloks (4mM) grubunda (67.22±3.71%), tüm gruplara kıyasla 72. saatte en yüksek canlılık sonucuna ulaşılmış ayrıca kontrol grubu (46.53±3.53) ile istatistiksel farklılık (p<0.05) belirlenmiştir. Çalışmanın sonucunda koç sperma sulandırıcısına eklenen BSA ve troloksun koç spermasının kısa süreli saklamasında faydalı olduğu ve spermatolojik parametreler üzerine koruyucu etki gösterdiği belirlenmiştir.

Keywords

• BSA
• kısa süreli saklama
• trehaloz
• troloks

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