Kidney tissue selenium levels of Toxocara canis infected mice given Nigella sativa

Year 2021, , 278 - 283, 31.12.2021

Serkan Sayıner , Mehtap Altaş , Güzin Camkerten , Ahmet Özer Şehirli , Nilgün Paksoy , Burcu Menekşe Balkan , Gülten Şenocak Soran

https://doi.org/10.31797/vetbio.977962

Cited By: 2

Abstract

Nigella sativa (NS) has a protective effect on cellular damage caused by oxidative stress. Selenium has an antioxidative effect. Toxocara canis is one of the nematodes causing visceral larva migrans. Men infected with this parasite ingesting an embryonic egg. It is more common in children between 1-4 years than adults. From the ingested embryonic egg, the larvae released in the small intestine and they migrate to so many organs such as liver, spleen, kidney, lung, brain, retina of the eye, pancreas and causing lesions. In particular, it is known to cause intense damage to kidney tissue. In this study mice with Toxocara canis infection were administered Nigella sativa in prophylactic and treatment doses (100 and 200 mg/kg body weight) and selenium levels were determined in their kidney tissues. In the healthy control group, kidney selenium levels were 980,46±236,68 ng/g and in mice infected with Toxocara canis 1240,15±315,77 ng/g. Kidney tissue Se levels of mice given NS in two different doses for treatment (Treated N100, Treated N200) and prophylaxis (Prophylactic N100 and ProphylacticN200) respectively are 1297,95±354,37; 1361,29±410,46 ng/g; 1148,55±240,28 ng/g and 1465,81±450,25 ng/g. Kidney tissue selenium levels were high in both treatment and prophylaxis dose NS given mice. In conclusion, Nigella sativa can cause increases in kidney tissue selenium levels depends on given doses.

Keywords

Nigella sativa, Selenium, Oxidative stress, Kidney, Toxocara canis

References

• Bowman, D.D., Mika-Grieve, M., Grieve, R.B. (1987). Circulating excretory-secretory antigen levels and specific antibody responses in mice infected with Toxocara canis. American Journal of Tropical Medicine and Hygiene, 36,75–82. https://doi.org/10.4269/ajtmh.1987.36.75
• Cheikh-Rouhou, S., Besbes, S., Hentati, B., Blekcer, C., Deroanne, C., Attia, H. (2007). Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction. Food Chemistry, 101, 673–681. https://doi.org/10.1016/j.foodchem.2006.02.022
• Combs, Jr G.F. (2015). Biomarkers of selenium status. Nutrients, 7, 2209–2236. https://doi.org/10.3390/nu7042209
• Darakhshan, S., Bidmeshki Pour, A., Hosseinzadeh Colagar, A., Sisakhtnezhad, S. (2015). Thymoquinone and its therapeutic potentials. Pharmacological Research, 95–96, 138–158. https://doi.org/10.1016/j.phrs.2015.03.011
• Ekor, M. (2014). The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology,4 JAN,1–10. https://doi.org/10.3389/fphar.2013.00177
• El-Refai, S.A., El-Aswad, B.E.D.W., Mahmoud, S.F. (2017). Parasitological, pathological and immunological effects of Nigella sativa oil in experimentally Toxocara canis infected mice. Tropical Biomedicine, 34, 781–794.
• Geerts, S., Gryseels, B. (2001). Anthelmintic resistance in human helminths: a review. Tropical Medicine & International Health, 6, 915–921. https://doi.org/10.1046/j.1365-3156.2001.00774.x
• Huang, Z., Rose, A.H., Hoffmann, P.R. (2012). The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities. Antioxidants & Redox Signaling, 16, 705–743. https://doi.org/10.1089/ars.2011.4145
• Jakaria, M., Cho, D.Y., Haque, M.E., Karthivashan, G., Kim, I.S., Ganesan, P., Choi, D.K. (2018). Neuropharmacological potential and delivery prospects of thymoquinone for neurological disorders. Oxidative Medicine and Cellular Longevity, 4, 1-17. https://doi.org/10.1155/2018/1209801
• Kappagoda, S., Singh, U., Blackburn, B.G. (2011). Antiparasitic therapy. Mayo Clinic Proceedings, 86 (6), 561–583. https://doi.org/10.4065/mcp.2011.0203
• Khaldi, T., Chekchaki, N., Boumendjel, M., Taibi, F., Abdellaoui, M., Boumendjel, A. (2018). Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxidative stress and cytotoxicity induced by smokeless tobacco extract in an allergic asthma model in Wistar rats. Allergologia et Immunopathologia, 46, 472–481. https://doi.org/10.1016/j.aller.2018.02.005
• Kleine, A., Springer, A., Strube, C. (2017). Seasonal variation in the prevalence of Toxocara eggs on children’s playgrounds in the city of Hanover, Germany. Parasites and Vectors, 10, 6–13. https://doi.org/10.1186/s13071-017-2193-6
• Koh, T.S., Benson, T.H. (1983). Critical re-appraisal of fluorometric method for determination of selenium in biological materials. Journal - Association of Official Analytical Chemists, 66, 918–926.
• Lobo, V., Patil, A., Phatak, A., Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4, 118–126. https://doi.org/10.4103/0973-7847.70902
• Lukác, N., Massányi, P. (2007). Effects of trace elements on the immune system. Epidemiologie, mikrobiologie, imunologie, 56, 3–9.
• Mahadappa, P., Dey, S. (2018). Effects of Toxocara canis Infection and Albendazole Treatment on Oxidative/Nitrosative Stress and Trace Element Status in Dogs. International Journal of Livestock Research, 8, 144–153. https://doi.org/10.5455/ijlr.20170720111145
• Mahmoud, M.R., El-Abhar, H.S., Saleh, S. (2002). The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. Journal of Ethnopharmacology, 79, 1–11. https://doi.org/10.1016/s0378-8741(01)00310-5
• Majdalawieh, A.F., Fayyad, M.W. (2015). Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review. International Immunopharmacology, 28, 295–304. https://doi.org/10.1016/j.intimp.2015.06.023
• Meral, I., Yener, Z., Kahraman, T., Mert, N. (2001). Effect of Nigella sativa on Glucose Concentration, Lipid Peroxidation, Anti-Oxidant Defence System and Liver Damage in Experimentally-Induced Diabetic Rabbits. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 48, 593–599. https://doi.org/10.1046/j.1439-0442.2001.00393.x
• Musa, D., Senocak, G., Borazan, G., Altaş, M., Ozgonul, A., Sogut, O., Güldür, M.E. (2011). Effects of Nigella sativa and albendazole alone and in combination in Toxocara canis infected mice. Journal of Pakistan Medical Association, 61, 866–870.
• Naz, H. (2011). Nigella sativa: the miraculous herb. Journal of Biochemistry and Molecular Biology, 44, 44–48. Pilarczyk, B., Jankowiak, D., Tomza-Marciniak, A., Pilarczyk, R., Sablik, P., Drozd, R., Tylkowska, A., Skólmowska, M. (2012). Selenium concentration and glutathione peroxidase (GSH-Px) activity in serum of cows at different stages of lactation. Biological Trace Element Research, 147, 91–96. https://doi.org/10.1007/s12011-011-9271-y
• Shalaby, H.A., El-Moghazy, F.M. (2013). In vitro effect of Nigella sativa oil on adult Toxocara vitulorum. Pakistan Journal of Biological Sciences, 16, 1557–1562. https://doi.org/10.3923/pjbs.2013.1557.1562
• Shamberger, R.J. (1983). Biochemistry of Selenium. New York: Plenum Publishing.
• Sun, Z., Xu, Z., Wang, D., Yao, H., Li, S. (2018). Selenium deficiency inhibits differentiation and immune function and imbalances the Th1/Th2 of dendritic cells. Metallomics, 10, 759–767. https://doi.org/10.1039/c8mt00039e
• Tapiero, H., Townsend, D.M., Tew, K.D. (2003). The antioxidant role of selenium and seleno-compounds. Biomedicine & Pharmacotherapy, 57, 134–144. https://doi.org/10.1016/s0753-3322(03)00035-0
• Tavakkoli, A., Ahmadi, A., Razavi, B.M., Hosseinzadeh H. (2017). Black Seed (Nigella Sativa) and its Constituent Thymoquinone as an Antidote or a Protective Agent Against Natural or Chemical Toxicities. Iranian Journal of Pharmaceutical Research, 16, 2–23.
• Tinggi, U. (2008). Selenium: its role as antioxidant in human health. Environmental Health and Preventive Medicine, 13, 102–108. https://doi.org/10.1007/s12199-007-0019-4
• Tülüce, Y., Özkol, H., Söǧüt, B., Çelik, I. (2009). Effects of Nigella sativa L. on lipid peroxidation and reduced glutathione levels in erythrocytes of broiler chickens. Cell Membranes and Free Radical Research, 1, 95–99.
• Yarsan, E., Altinsaat, Ç., Ayçiçek, H., Şahindokuyucu, F., Kalkan, F. (2003). Effects of Albendazole Treatment on Haematological and Biochemical Parameters in Healthy and Toxocara canis Infected Mice.Turkısh Journal Of Veterınary and Anımal Scıences, 27,1057–1063.