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Yavru Gökkuşağı alabalıkları (Oncorhynchus mykiss, W.)’ndan Lactococcus garvieae’nin İzolasyonu ve Plazmit Profilleri Üzerine Bir Çalışma

Yıl 2018, Cilt: 3 Sayı: 1, 11 - 22, 30.04.2018
https://doi.org/10.31797/vetbio.401284

Öz

Bu çalışmada Fethiye bölgesindeki bazı gökkuşağı alabalığı (Oncorhynchus mykiss) işletmelerindeki hasta yavru balıklardan Lactococcus garvieae suşları izole ve identifiye edilmiştir. Hasta balıklarda durgunluk, iştahsızlık, gözlerde ekzoftalmi ve hemoraji, abdominal kısımda şişkinlik gözlenmiştir. İç bulgu olarak karaciğerde hemoraji, dalakta büyüme (splenomegali) ve karın boşluğunda asidik sıvı birikimi tespit edilmiştir. Lactococcus garvieae suşları ampisilin, eritromisin, kloramfenikol, sülfametoksazol, oksitetrasiklin, tetrasiklin ve trimetoprim’e duyarlılık, basitrasin, flumekuin, furazolidon, kanamisin, nalidiksik asit, oksalinik asit ve streptomisin’e direnç göstermiştir. Plazmit analiz sonuçlarına göre, suşların farklı boyutlarda plazmitleri içerdikleri bulunmuştur.

Kaynakça

  • Anderson, D. G., McKay, L. L. (1983). A simple and rapid method for isolation large plasmid DNA from lactic streptococci. Appl Environ Microbiol., 46(3), 549-552.
  • Aoki, T., Takami, K., Kitao, T. (1990). Drug resistance in a non-hemolytic Streptococcus sp. isolated from cultured yellowtail Seriola quinqueradiata. Dis Aquat Org., 8, 171-7.
  • Arda, M. (1997). Temel Mikrobiyoloji. Ankara: Medisan Yayın No: 25.
  • Avcı, H., Aydoğan, A., Tanrıkul, T. T., Birincioğlu, S. S. (2010). Pathological and microbiological investigations in rainbow trout (Oncorhycnhus mykiss Walbaum, 1792) naturally infected with Lactococcus garvieae. Kafkas Univ Vet Fak Derg., 16, 313-18. DOI: 10.9775/kvfd.2010.2452.
  • Altun, S., Diler, Ö., Adiloğlu, K. (2004). Genotyping of Lactococcus garvieae strains from rainbow trout (Oncorhynchus mykiss) by 16s RDNA sequencing. Bull Eur Ass Fish Pathol., 24, 119-125.
  • Bounaix, S., Benachour, a., Geerges, N. (1996). Presence of lactose genes and insertion sequences in plasmids of minor species of the Genus Lactococcus. Appl Environ Microbiol., 62(3), 1112-1115.
  • Buller, N. B. (2004). Bacteria from fish and other aquatic animals: A practical identification manual. UK: CABI Publishing.
  • CLSI (Clinical and laboratory Standards Institute) (2006). Temel Mikrobiyoloji. Ankara: Medisan yayın No: 25.
  • Çağırgan, H., Tanrıkul, T. T. (1997). A Lactococcus in a trout farm. Mediterranean Fisheries Congress, İzmir, 40.
  • Didinen, B. I., Yardımcı, B., Onuk, E. E., Metin, S., Yıldırım, P. (2014). Naturally Lactococcus garvieae infection in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) new histopathological observations, phenotypic and molecular identification. Revue Méd Vét., 165(1-2), 12-19.
  • Diler, O., Altun, S., Adiloğlu, K. A., Kubilay, A., Işıklı, B. (2002). First occurence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull Eur Ass Fish Pathol., 22, 21-6.
  • Fukushima, H. C. S., Bailone R. L., Ranzani Paiva, M. J. T. (2017). Lactococcosis reared fish in Brazil and control strategies. J Vaccines Vaccin., 8, 1-3. DOI: 10.4172/2157-7560.1000357.
  • Izumi S., Aranishi, F. (2004). Plasmid profilling of japanese Flavobacterium psychrophilum isolates. J Aquat Anim Health, 16, 99-103.
  • Kav, K., Erganiş, O. (2008). Antibiotic susuceptibility of Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss) farms. Bull Vet Inst Pulawy, 52, 223-226.
  • Kitao, T. (1993). Streptococcal infections. In: Inglis V., Roberts RJ, Bromage NR(ed’s). Bacterial Diseases of Fish. Blackwell Sciences Ltd, Cambridge, UK, 196-210.
  • Kubilay, A., Altun, S., Uluköy, G., Diler, Ö. (2005). Lactococcus garvieae suşlarının antimikrobiyal duyarlılıklarının belirlenmesi. SDU-ESUFD, 1(1), 39-48.
  • Kuk, S., Erensoy, A. (2008). Gen klonlama, plazmit seçimi ve Fasciola hepatica Cathepsin L1 uygulamaları. Türkiye Parazitol Derg., 32(1), 16-22.
  • Maki, T., Hirano, I., Aoki, I. (2008). Drug resistance mechanism of the fish pathogenic bacterium Lactococcus garvieae. J Fish Dis., 31, 461-468.
  • Mazzolini, E., Vismara, D., Ceschia, G., Malvisi, J., Fabris, A., Passera, A., Danielis, L., Giorgetti, G. (2003). In vitro activity of several chemiantibiotics against clinical isolates of fresh and marine fish pathogens. Appl Environ Microbiol., 77, 2114-2185.
  • Menéndez, A., Fernandez, L., Reimundo, P., Guijarro, J. A. (2007). Genes required for Lactococcus garvieae survival in a fish host. Microbiology, 153, 3286-3294.
  • Meyburgh, C. M., Bragg, R. R., Boucher, C. E. (2017). Lactococcus garvieae: an emerging bacterial pathogen of fish. Dis Aquat Org., 123 (1), 67-79. DOI: 10.3354/dao03083.
  • NCLS (2003). Disk Difüzyon Ek Tablolar, M100-S13 (M2). İstanbul: Türk Mikrobiyoloji Cemiyeti.
  • Öztürk, T., Didinen, B. I., Doğan, G., Özer, A., Bircan, R. (2009). Gökkuşağı alabalığında, (Oncorhynchus mykiss, W., 1792) lactococcosis hastalığı ve Lactococcus garvieae’nin antibakteriyel duyarlılığı. Ulusal Su Günleri 2009 Sempozyumu, Elazığ.
  • Pridgeon, J. W., Klesius, P. H. (2012). Major bacterial diseases in aquaculture and their vaccine development. CAB Reviews, 7 (48), 1-16. DOI: 10.1079/PAVSNNR20127048.
  • Savvidis, G. K., Anatoliotis, C., Kanaki, Z., Vafeas G. (2007). Epizootic outbreaks of lactococcosis disease in rainbow trout, O. mykiss (Walbaum), culture in Greece. Bull Eur Ass Fish Pathol., 28(5), 223-8.
  • Shin, G. W., Palaksha, H. H., Yang, Y. S., Shin, Y. S., Kim, Y. R., Lee, E. Y., Kim, H. Y., OH, M. J., Yoshida, T., Jung, T. S. (2006). Discrimination of streptococcosis agents in olive flounder (Paralichthys olivaceus). Bull Eur Ass Fish Pathol., 26, 68-79.
  • Soltani, M., Nikbakht, Gh., Mousavi, H. A. E., Ahmadzadeh, N. (2008). Epizootic outbreaks of lactococcosis caused by Lactococcus garvieae in farmed rainbow trout (Oncorhynchus mykiss) in Iran. Bull Eur Ass Fish Pathol., 28(5), 170-175.
  • Søorum, H., Olsuiko, P. (1998). Plasmids in Vibrio salmonicida isolated from salmonids with haemorrhagic syndrome (Hitra disease). J Clin Microbiol., 26(9), 1679-1683.
  • Timur, G., Timur, M. (2003). Balık Hastalıkları. İstanbul: İstanbul Üniversitesi Su Ürünleri Fakültesi Yayın No: 5.
  • Toranzo, A. E., Combarro, P., Lemas, M. L., Barja, J. L. (1994). Plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout. Appl Environ Microbiol., 48(4), 872-877.
  • Türe, M., Alp, H. (2016). Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey. J Vet Res, 60, 141-6. DOI: 10.1515/jvetres-2016-0020.
  • Vendrell, D., Balcazar, J. L., Ruiz-Zarzuela, I., de Blas, I., Girones, O., Muzquiz, J. L. (2006). Lactococcus garvieae in fish: a review. Comp Immun Microbial Infect., 29, 177-198.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology, Manual Techniques and Procedures. USA: A Blackwell Publishing Company.

A Study on Plasmid Profiles and Isolation of Lactococcus garvieae from the Young Rainbow Trout (Oncorhynchus mykiss, W.)

Yıl 2018, Cilt: 3 Sayı: 1, 11 - 22, 30.04.2018
https://doi.org/10.31797/vetbio.401284

Öz



In this study, Lactococcus garvieae strains were isolated and identified from sick young fish from some rainbow trout (Oncorhynchus mykiss) farms in Fethiye region. Lethargy, anorexia, exophthalmi with hemorrhage and abdominal dropsy in the sick fish were observed. Hemorrhage in the liver, enlargement of the spleen (splenomegaly) and ascites as internal signs were determined. The strains showed sensitivity to ampicillin, erythromycine, chloramphenicol, sulfamethoxazole, oxytetracycline, tetracycline and trimethoprim and resistant to basitrasin, flumequine, furazolidone, kanamycin, nalidixic acid, oxalinic acid and streptomycine. It was found that the strains harboured plasmids with different sizes according to the results of plasmid analysis results.

Kaynakça

  • Anderson, D. G., McKay, L. L. (1983). A simple and rapid method for isolation large plasmid DNA from lactic streptococci. Appl Environ Microbiol., 46(3), 549-552.
  • Aoki, T., Takami, K., Kitao, T. (1990). Drug resistance in a non-hemolytic Streptococcus sp. isolated from cultured yellowtail Seriola quinqueradiata. Dis Aquat Org., 8, 171-7.
  • Arda, M. (1997). Temel Mikrobiyoloji. Ankara: Medisan Yayın No: 25.
  • Avcı, H., Aydoğan, A., Tanrıkul, T. T., Birincioğlu, S. S. (2010). Pathological and microbiological investigations in rainbow trout (Oncorhycnhus mykiss Walbaum, 1792) naturally infected with Lactococcus garvieae. Kafkas Univ Vet Fak Derg., 16, 313-18. DOI: 10.9775/kvfd.2010.2452.
  • Altun, S., Diler, Ö., Adiloğlu, K. (2004). Genotyping of Lactococcus garvieae strains from rainbow trout (Oncorhynchus mykiss) by 16s RDNA sequencing. Bull Eur Ass Fish Pathol., 24, 119-125.
  • Bounaix, S., Benachour, a., Geerges, N. (1996). Presence of lactose genes and insertion sequences in plasmids of minor species of the Genus Lactococcus. Appl Environ Microbiol., 62(3), 1112-1115.
  • Buller, N. B. (2004). Bacteria from fish and other aquatic animals: A practical identification manual. UK: CABI Publishing.
  • CLSI (Clinical and laboratory Standards Institute) (2006). Temel Mikrobiyoloji. Ankara: Medisan yayın No: 25.
  • Çağırgan, H., Tanrıkul, T. T. (1997). A Lactococcus in a trout farm. Mediterranean Fisheries Congress, İzmir, 40.
  • Didinen, B. I., Yardımcı, B., Onuk, E. E., Metin, S., Yıldırım, P. (2014). Naturally Lactococcus garvieae infection in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) new histopathological observations, phenotypic and molecular identification. Revue Méd Vét., 165(1-2), 12-19.
  • Diler, O., Altun, S., Adiloğlu, K. A., Kubilay, A., Işıklı, B. (2002). First occurence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull Eur Ass Fish Pathol., 22, 21-6.
  • Fukushima, H. C. S., Bailone R. L., Ranzani Paiva, M. J. T. (2017). Lactococcosis reared fish in Brazil and control strategies. J Vaccines Vaccin., 8, 1-3. DOI: 10.4172/2157-7560.1000357.
  • Izumi S., Aranishi, F. (2004). Plasmid profilling of japanese Flavobacterium psychrophilum isolates. J Aquat Anim Health, 16, 99-103.
  • Kav, K., Erganiş, O. (2008). Antibiotic susuceptibility of Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss) farms. Bull Vet Inst Pulawy, 52, 223-226.
  • Kitao, T. (1993). Streptococcal infections. In: Inglis V., Roberts RJ, Bromage NR(ed’s). Bacterial Diseases of Fish. Blackwell Sciences Ltd, Cambridge, UK, 196-210.
  • Kubilay, A., Altun, S., Uluköy, G., Diler, Ö. (2005). Lactococcus garvieae suşlarının antimikrobiyal duyarlılıklarının belirlenmesi. SDU-ESUFD, 1(1), 39-48.
  • Kuk, S., Erensoy, A. (2008). Gen klonlama, plazmit seçimi ve Fasciola hepatica Cathepsin L1 uygulamaları. Türkiye Parazitol Derg., 32(1), 16-22.
  • Maki, T., Hirano, I., Aoki, I. (2008). Drug resistance mechanism of the fish pathogenic bacterium Lactococcus garvieae. J Fish Dis., 31, 461-468.
  • Mazzolini, E., Vismara, D., Ceschia, G., Malvisi, J., Fabris, A., Passera, A., Danielis, L., Giorgetti, G. (2003). In vitro activity of several chemiantibiotics against clinical isolates of fresh and marine fish pathogens. Appl Environ Microbiol., 77, 2114-2185.
  • Menéndez, A., Fernandez, L., Reimundo, P., Guijarro, J. A. (2007). Genes required for Lactococcus garvieae survival in a fish host. Microbiology, 153, 3286-3294.
  • Meyburgh, C. M., Bragg, R. R., Boucher, C. E. (2017). Lactococcus garvieae: an emerging bacterial pathogen of fish. Dis Aquat Org., 123 (1), 67-79. DOI: 10.3354/dao03083.
  • NCLS (2003). Disk Difüzyon Ek Tablolar, M100-S13 (M2). İstanbul: Türk Mikrobiyoloji Cemiyeti.
  • Öztürk, T., Didinen, B. I., Doğan, G., Özer, A., Bircan, R. (2009). Gökkuşağı alabalığında, (Oncorhynchus mykiss, W., 1792) lactococcosis hastalığı ve Lactococcus garvieae’nin antibakteriyel duyarlılığı. Ulusal Su Günleri 2009 Sempozyumu, Elazığ.
  • Pridgeon, J. W., Klesius, P. H. (2012). Major bacterial diseases in aquaculture and their vaccine development. CAB Reviews, 7 (48), 1-16. DOI: 10.1079/PAVSNNR20127048.
  • Savvidis, G. K., Anatoliotis, C., Kanaki, Z., Vafeas G. (2007). Epizootic outbreaks of lactococcosis disease in rainbow trout, O. mykiss (Walbaum), culture in Greece. Bull Eur Ass Fish Pathol., 28(5), 223-8.
  • Shin, G. W., Palaksha, H. H., Yang, Y. S., Shin, Y. S., Kim, Y. R., Lee, E. Y., Kim, H. Y., OH, M. J., Yoshida, T., Jung, T. S. (2006). Discrimination of streptococcosis agents in olive flounder (Paralichthys olivaceus). Bull Eur Ass Fish Pathol., 26, 68-79.
  • Soltani, M., Nikbakht, Gh., Mousavi, H. A. E., Ahmadzadeh, N. (2008). Epizootic outbreaks of lactococcosis caused by Lactococcus garvieae in farmed rainbow trout (Oncorhynchus mykiss) in Iran. Bull Eur Ass Fish Pathol., 28(5), 170-175.
  • Søorum, H., Olsuiko, P. (1998). Plasmids in Vibrio salmonicida isolated from salmonids with haemorrhagic syndrome (Hitra disease). J Clin Microbiol., 26(9), 1679-1683.
  • Timur, G., Timur, M. (2003). Balık Hastalıkları. İstanbul: İstanbul Üniversitesi Su Ürünleri Fakültesi Yayın No: 5.
  • Toranzo, A. E., Combarro, P., Lemas, M. L., Barja, J. L. (1994). Plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout. Appl Environ Microbiol., 48(4), 872-877.
  • Türe, M., Alp, H. (2016). Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey. J Vet Res, 60, 141-6. DOI: 10.1515/jvetres-2016-0020.
  • Vendrell, D., Balcazar, J. L., Ruiz-Zarzuela, I., de Blas, I., Girones, O., Muzquiz, J. L. (2006). Lactococcus garvieae in fish: a review. Comp Immun Microbial Infect., 29, 177-198.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology, Manual Techniques and Procedures. USA: A Blackwell Publishing Company.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Hidrobiyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Jale Korun

Mehtap Kurtoğlu Bu kişi benim

Yayımlanma Tarihi 30 Nisan 2018
Gönderilme Tarihi 4 Mart 2018
Kabul Tarihi 26 Nisan 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 3 Sayı: 1

Kaynak Göster

APA Korun, J., & Kurtoğlu, M. (2018). Yavru Gökkuşağı alabalıkları (Oncorhynchus mykiss, W.)’ndan Lactococcus garvieae’nin İzolasyonu ve Plazmit Profilleri Üzerine Bir Çalışma. Journal of Advances in VetBio Science and Techniques, 3(1), 11-22. https://doi.org/10.31797/vetbio.401284

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