BVDV monitoring by pooling and real time RT-PCR as economical monitoring technique with low BVDV prevalence

Yıl 2022, Cilt: 7 Sayı: 1, 72 - 79, 30.04.2022

Hikmet Ün Mustafa Gökçe Oğuz Ayaz Suna Simsek Osman Karabulut

https://doi.org/10.31797/vetbio.1072218

Öz

The aim of this study was to determine whether the use of pooled blood samples and real-time RT-PCR are feasible for detecting BVDV in viremic or PI animals. For this purpose, blood samples obtained from 2701 cattle, brought from 62 different farms, were used to test for the presence of bovine viral diarrhoea virus (BVDV) at the Veterinary Virology Department of Aksaray University Faculty of Veterinary Medicine. The sampling was done from all geographical regions in Turkey. Blood samples were pooled in groups of eight, creating a total of 342 pools. Each pool was checked for BVDV with a real-time RT-PCR test. BVDV nucleic acid was detected in 18 (5.26%) of a total of 342 pools. BVDV was detected in 18 of 62 farms. The most important result obtained from this study is that BVDV monitoring by pooling and real time RT-PCR can be done very economically when the disease prevalence is low (<10%). A risk/benefit estimation can be done for breeders who want to start vaccination programs.

Anahtar Kelimeler

BVDV, Pooling, Real Time RT-PCR

Destekleyen Kurum

MSD Hayvan Sağlığı, Türkiye (https://www.msd-hayvan-sagligi.com/) ve Diagen Biyoteknolojik Sistemler Sağlık Hizmetleri ve Otomasyonu San. Tic. A.Ş., Ankara (www.diagen.com.tr).

Teşekkür

This work was supported by MSD Animal Health, Turkey (https://www.msd-hayvan-sagligi.com/) and Diagen Biyoteknolojik Sistemler Sağlık Hizmetleri ve Otomasyonu San. Tic. A.Ş., Ankara (www.diagen.com.tr). The authors would like to acknowledge Ahmet Enis Yağar for his assistance with laboratory procedures.

Düşük BVDV prevalansı ile seyreden olguların izlenmesinde ekonomik bir izleme tekniği olarak havuzlama ve gerçek zamanlı RT-PZR

Öz

Bu çalışmanın amacı, havuzlanmış örnek ve gerçek zamanlı RT-PCR kullanımının viremik veya PI hayvanlarda BVDV'yi tespit etmek için uygun olup olmadığının belirlenmesidir. Bu amaçla, Aksaray Üniversitesi Veteriner Fakültesi Teşhis ve Analiz Laboratuvarına bovine viral diarrhoea virus (BVDV) enfeksiyonu açısından test edilmek üzere 62 farklı çiftlikten getirilen, 2701 sığırdan sağlanan kan örneği kullanıldı. Türkiye’nin tüm cografi bölgelerinden örnekleme yapıldı. Kan örnekleri sekizli gruplar halinde havuzlandı, toplam 342 adet havuz oluşturuldu. Her bir havuz BVDV yönünden real time RT-PCR testi ile kontrol edildi. Toplam 342 havuzdan 18 adedinde (%5,26) BVDV antijeni saptandı. Kontrol edilen 62 çiftliğin 18 tanesinde virus tespit edildi. Bu araştırmadan elde edilen en önemli sonuç, hastalık prevalansının düşük (<%10) olduğu durumlarda havuzlama ve real time RT-PCR ile BVDV izlemesinin çok ekonomik olarak yapılabileceğinin gösterilmesidir. Aşılama programlarına başlamak isteyen yetiştiriciler için bir risk / fayda tahmini yapılabilir.

Anahtar Kelimeler

BVDV, Havuzlama, Gerçek Zamanlı RT-PZR

Kaynakça

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