The effect of lycopene and ascorbic acid on the post thaw Angora buck semen parameters

Year 2024, Volume: 9 Issue: 2, 133 - 142, 22.08.2024

Mustafa Bodu , Ali Erdem Öztürk , Zeliha Kılınç , Ömer Hatipoğlu , Mehmet Bozkurt Ataman , Mustafa Numan Bucak , Mustafa Kul

https://doi.org/10.31797/vetbio.1489224

Abstract

The main goal of this research was to examine the impact of antioxidants, specifically lycopene and ascorbic acid, on the viability of goat sperm following cryopreservation and thawing. Goat rearing plays a vital role worldwide by supplying animal protein, creating employment opportunities, and providing raw materials for various industries. Improving the reproductive efficiency of goats is crucial for genetic conservation, as well as for enhancing profitability and sustainability for breeders. This study illustrates that both lycopene and ascorbic acid, either alone or in combination, have a positive influence on motility, plasma, acrosomal, and mitochondrial membrane integrity after thawing. Semen samples were obtained from four mature goats and segregated into four groups: control (C), lycopene at 2 mM/mL (L2), ascorbic acid at 5 mM/mL (A5), and a combination of lycopene at 2 mM/mL + ascorbic acid at 5 mM/mL (L2A5). Following dilution in Tris/egg yolk diluent, the semen samples were cryopreserved in liquid nitrogen and then thawed for assessment. The L2A5 group displayed the highest values across all evaluated parameters (motility, plasma, mitochondrial and acrosomal membrane integrity) when compared to the control group (p<0.05). These results indicate that the concurrent use of lycopene and ascorbic acid can significantly enhance the quality of cryopreserved goat semen, thereby contributing to improved reproductive outcomes and genetic preservation in goats.

Keywords

Ascorbic acid, buck, cryopreservation, lycopene, semen

Ethical Statement

Selcuk University Faculty of Veterinary Medicine Experimental Animal Production and Research Center Ethics Committee (SÜDAVMEK) Meeting Date: 02.05.2024 Number of Meeting: 2024/04 Number of Decision: 2024/085

References

• Agarwal, A., & Majzoub, A. (2017). Laboratory tests for oxidative stress. Indian Journal of Urology, 33(3), 199-206. https://doi.org/10.4103/iju.IJU_9_17
• Aitken, R. J. (2020). Impact of oxidative stress on male and female germ cells: implications for fertility. Reproduction, 159(4), R189-R201. https://doi.org/10.1530/REP-19-0452
• Akalin, P. P., Bucak, M. N., Güngör, Ş., Başpinar, N., Coyan, K., Dursun, Ş., ... & Deniz, Y. E. N. İ. (2016). Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 C. Small Ruminant Research, 137, 117-123. https://doi.org/10.1016/j.smallrumres.2016.03.017 Akhter, S., Zubair, M., Mahmood, M., Andrabi, S. M. H., Hameed, N., Ahmad, E., & Saleemi, M. K. (2023). Effects of vitamins C and E in tris citric acid glucose extender on chilled semen quality of Kail ram during different storage times. Scientific Reports, 13(1), 18123. https://doi.org/10.1038/s41598-023-43831-2
• Al-Mutary, M. G. (2021). Use of antioxidants to augment semen efficiency during liquid storage and cryopreservation in livestock animals: A review. Journal of King Saud University-Science, 33(1), 101226. https://doi.org/10.1016/j.jksus.2020.10.023
• Amini, M. R., Kohram, H., Zare Shahaneh, A., Zhandi, M., Sharideh, H., & Nabi, M. M. (2015). The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post thawed sperm quality. Cell and Tissue Banking, 16, 587-592. https://doi.org/10.1007/s10561-015-9506-9
• Babaei, A., Asadpour, R., Mansouri, K., Sabrivand, A., & Kazemi-Darabadi, S. (2021). Lycopene protects sperm from oxidative stress in the experimental varicocele model. Food Science & Nutrition, 9, 6806–6817. https://doi.org/10.1002/fsn3.2632
• Bechara, N., Flood, V. M., & Gunton, J. E. (2022). A systematic review on the role of vitamin C in tissue healing. Antioxidants, 11(8), 1605. https://doi.org/10.3390/antiox11081605
• Bintara, S., Maharani, D., Tavares, L., & Sitaresmi, P. (2023). Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram. Journal of Animal Science and Technology. https://doi.org/10.5187/jast.2023.e54
• Bucak, M. N., Ataman, M. B., Başpınar, N., Uysal, O., Taşpınar, M., Bilgili, A., ... & Akal, E. (2015). Lycopene and resveratrol improve post‐thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia, 47(5), 545-552. https://doi.org/10.1111/and.12301
• Bucak, M. N., Bodu, M., Başpınar, N., Güngör, Ş., İli, P., Acibaeva, B., ... & Dursun, Ş. (2019). Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Cell Journal (Yakhteh), 21(1), 7. https://doi.org/10.22074/cellj.2019.5593
• Bucak, M. N., Karaşör, Ö. F., Sarı, A., Bodu, M., Ili, P., Narlıçay, S., ... & Sari, F. (2024). Lipid mixtures (from a liposome kit) and melatonin improve post-thawed Angora goat sperm parameters. Cryobiology, 115, 104897. https://doi.org/10.1016/j.cryobiol.2024.104897
• Bucak, M. N., Keskin, N., Ili, P., Bodu, M., Akalın, P. P., Öztürk, A. E., ... & Dursun, Ş. (2020). Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses. Cryobiology, 96, 19-29. https://doi.org/10.1016/j.cryobiol.2020.09.001
• Bucak, M. N., Tuncer, P. B., Sarıözkan, S., & Ulutaş, P. A. (2009). Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities. Small Ruminant Research, 81(1), 13-17. https://doi.org/10.1016/j.smallrumres.2008.10.003
• Bucak, M.N., Ataman, M.B., Başpınar, N., Uysal, O., Taşpınar, M., Bilgili, A., Öztürk, C., Güngör, Ş., İnanç, M.E. and Akal, E. (2015). Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia, 47, 545-552. https://doi.org/10.1111/and.12301
• Buettner, G. R. (1993). The pecking order of free radicals and antioxidants: lipid peroxidation, α-tocopherol, and ascorbate. Archives of Biochemistry and Biophysics, 300(2), 535-543. https://doi.org/10.1006/abbi.1993.1074
• Evans, G., & Maxwell, W. C. (1987). Salamons' artificial insemination of sheep and goats (No. Ed. 2, pp. xi+-194).
• Garner, D. L., & Johnson, L. A. (1995). Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biology of Reproduction, 53(2), 276-284. https://doi.org/10.1095/biolreprod53.2.276
• Garner, D. L., Thomas, C. A., Joerg, H. W., DeJarnette, J. M., & Marshall, C. E. (1997). Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biology of Reproduction, 57(6), 1401-1406. https://doi.org/10.1095/biolreprod57.6.1401
• Hussein, A., Aseffa, S., Kuraz, B., & Bedaso, B. (2023). Breeding and milking managements and Goat production constraints in Siltie Zone SNNPR, Ethiopia. Heliyon, 9(12). https://doi.org/10.1016/j.heliyon.2023.e22573
• Karaşör, Ö. F., Bucak, M. N., Cenariu, M., Bodu, M., Taşpınar, M., & Taşpınar, F. (2022). The effects of different doses of ROCK inhibitor, antifreeze protein III, and boron added to semen extender on semen freezeability of Ankara bucks. Molecules, 27(22), 8070. https://doi.org/10.3390/molecules27228070
• Khalique, M. A., Rehman, H., Andrabi, S. M. H., Majeed, K. A., Ahmad, N., Fayyaz, M. H., ... & Sulaiman, S. (2023). Antioxidant effects of zinc-oxide nanoparticles on post-thaw quality and in vivo fertility of Beetal buck spermatozoa. Small Ruminant Research, 225, 107012. https://doi.org/10.1016/j.smallrumres.2023.107012
• Memon, A., Wahid, H., Rosnina, Y., Goh, Y., Ebrahimi, M. and Nadia, F. (2013). Effect of Ascorbic Acid Concentrations, Methods of Cooling and Freezing on Boer Goat Semen Cryopreservation. Reproduction in Domestic Animals, 48, 325-330. https://doi.org/10.1111/j.1439-0531.2012.02155.x
• Mishra, B., Alam, M. G. S., Khandokar, M. A. M. Y., Mazumder, S., & Munsi, M. N. (2010). Qualities of goat semen in Tris-Citrate-Glucose extender containing glutathione. The Bangladesh Veterinarian, 27(2), 46 – 55. https://doi.org/10.3329/bvet.v27i2.7554
• Nagy, S., Jansen, J., Topper, E. K., & Gadella, B. M. (2003). A triple-stain flow cytometric method to assess plasma-and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biology of Reproduction, 68(5), 1828-1835. https://doi.org/10.1095/biolreprod.102.011445
• Narliçay, S., & Uslu, B. A. (2022). Kangal Akkaraman koçu spermalarının seminal plazmasına farklı isı uygulamalarının kısa süreli saklanmasında etkilerinin araştırılması. Cumhuriyet Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 7(2), 112-117. https://doi.org/10.51754/cusbed.1115552
• Paudel, K., Kumar, S., Meur, S. and Kumaresan, A. (2010). Ascorbic Acid, Catalase and Chlorpromazine Reduce Cryopreservation-induced Damages to Crossbred Bull Spermatozoa†. Reproduction in Domestic Animals, 45, 256-262. https://doi.org/10.1111/j.1439-0531.2008.01278.x
• Pellicer-Rubio, M. T., Magallon, T., & Combarnous, Y. (1997). Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity. Biology of Reproduction, 57(5), 1023-1031. https://doi.org/10.1095/biolreprod57.5.1023
• Purdy, P. H. (2006). A review on goat sperm cryopreservation. Small ruminant research, 63(3), 215 225. https://doi.org/10.1016/j.smallrumres.2005.02.015
• Raheem, K. A., Basiru, A., Raji, L. O., & Odetokun, I. A. (2024). Productive performance of goat. Trends in Clinical Diseases, Production and Management of Goats, 163-177. https://doi.org/10.1016/B978-0-443-23696-9.00001-8
• Ren, F., Feng, T., Dai, G., Wang, Y., Zhu, H., & Hu, J. (2018). Lycopene and alpha-lipoic acid improve semen antioxidant enzymes activity and cashmere goat sperm function after cryopreservation. Cryobiology, 84, 27-32. https://doi.org/10.1016/j.cryobiol.2018.08.006
• Rosato, M. P., Di Iorio, M., Manchisi, A., Gambacorta, M., Petrosino, G., Centoducati, G., ... & Iaffaldano, N. (2012). In vitro survival and lipid peroxidation status of rabbit spermatozoa after both chilled and frozen storage in lycopene enriched extenders. Livestock Science, 146(2-3), 199-202. https://doi.org/10.1016/j.livsci.2012.03.006
• Sen, S. (2019). The chemistry and biology of lycopene: Antioxidant for human health. International Journal of Advancement in Life Sciences Research, 2(4), 08-14. https://doi.org/10.31632/ijalsr.2019v02i04.002
• Shah, S. A. H., Andrabi, S. M. H., & Qureshi, I. Z. (2016). Effect of equilibration times, freezing, and thawing rates on post‐thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology, 4(5), 972 976. https://doi.org/10.1111/andr.12214
• Sheikholeslami, S. A., Soleimanzadeh, A., Rakhshanpour, A., & Shirani, D. (2020). The evaluation of lycopene and cysteamine supplementation effects on sperm and oxidative stress parameters during chilled storage of canine semen. Reproduction in Domestic Animals, 55(9), 1229-1239. https://doi.org/10.1111/rda.13770
• Sias, B., Ferrato, F., Pellicer-Rubio, M. T., Forgerit, Y., Guillouet, P., Leboeuf, B., & Carrière, F. (2005). Cloning and seasonal secretion of the pancreatic lipase-related protein 2 present in goat seminal plasma. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1686(3), 169-180. https://doi.org/10.1016/j.bbalip.2004.09.008
• Sies, H., Stahl, W., & Sundquist, A. R. (1992). Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Annals of the New York Academy of Sciences, 669, 7-20. https://doi.org/10.1111/j.1749-6632.1992.tb17085.x
• Simon, L., Zini, A., Dyachenko, A., Ciampi, A., & Carrell, D. T. (2017). A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian Journal of Andrology, 19(1), 80-90. https://doi.org/10.4103/1008-682X.182822
• Stahl, W., & Sies, H. (1996). Perspectives in biochemistry and biophysics. Archives of Biochemistry and Biophysics, 336(1), 1-9.
• Tuncer, P. B., Büyükleblebici, S., Eken, A., Taşdemir, U., Durmaz, E., Büyükleblebici, O., & Çoşkun, E. (2014). Comparison of cryoprotective effects of lycopene and cysteamine in different cryoprotectants on bull semen and fertility results. Reproduction in Domestic Animals, 49(5), 746-752. https://doi.org/10.1111/rda.12359
• Uysal, O., & Bucak, M. N. (2007). Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Veterinaria Brno, 76(3), 383-390. https://doi.org/10.2754/avb200776030383
• Velmurugan, B., Bhuvaneswari, V., Abraham, S. K., & Nagini, S. (2004). Protective effect of tomato against N-methyl-N′-nitro-N-nitrosoguanidine–induced in vivo clastogenicity and oxidative stress. Nutrition, 20(9), 812-816. https://doi.org/10.1016/j.nut.2004.05.003
• Zini, A., San Gabriel, M., & Libman, J. (2010). Lycopene supplementation in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Fertility and Sterility, 94(3), 1033-1036. https://doi.org/10.1016/j.fertnstert.2009.04.004