Diagnostic efficiency of miR-21 and miR-34a serum levels in malign and benign prostate diseases

Abstract

Objective: In this study aimed to determine the diagnostic efficiency of miR-21 and miR-34a serum levels in the discrimination of benign prostatic hyperplasia, chronic prostatitis, and prostate cancer.
Materials and Methods: Blood samples were taken from 70 patients (25 benign prostatic hyperplasias, 10 chronic prostatitides, and 35 prostate cancer) who underwent prostate needle biopsy. After obtaining serum under suitable conditions, RNA isolation, cDNA synthesis, and qRT-PCR analysis were performed using Qiagen brand kits on Rotor-Gene® Q (Qiagen, Germany) device. -∆Ct values ​​were calculated using RNU6 as a reference gene for normalization. -∆Ct values ​​were used in all statistical calculations.
Results: It was observed that miR-21 serum levels were upregulated in chronic prostatitis and cancer groups compared to benign prostatic hyperplasia and the difference between the groups was statistically significant (p = 0.021 and p = 0.001, respectively). The specificity and sensitivity of miR-21 and miR-21/miR-34a combination was calculated as 56% and 86%; 84% and 71% in discriminating benign prostatic hyperplasia and prostate cancer groups, respectively.
Conclusion: In this study, it has been shown that miR-21 and miR-21/miR-34a combination has diagnostic performance that can be a biomarker candidate in diagnosing prostate cancer. In addition, the presence of a gradual increase in chronic prostatitis and prostate cancer at miR-21 levels compared to benign prostatic hyperplasia suggests that inflammation and cancer transformation processes taking place at the molecular level are also reflected in the circulating microRNA profile.

Keywords

• Prostate cancer
• Prostatitis
• Benign prostatic hyperplasia
• MicroRNA
• Diagnostic efficiency

Malign ve benign prostat hastalıklarında miR-21 ve miR-34a serum düzeylerinin tanısal etkinliği

Abstract

Amaç: Bu çalışmada, benign prostat hiperplazisi, kronik prostatit ve prostat kanseri ayrımında miR-21 ve miR-34a serum seviyelerinin tanısal etkinliğinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntemler: Prostat iğne biyopsisi yapılan toplamda 70 hastadan (25 benign prostat hiperplazisi, 10 kronik prostatit ve 35 prostat kanseri) kan örnekleri alındı. Uygun koşullarda serum eldesinden sonra RNA izolasyonu, cDNA sentezi ve qRT-PCR analizi Rotor-Gene® Q (Qiagen, Germany) cihazında Qiagen marka kitler kullanılarak gerçekleştirildi. Normalizasyon için referans gen olarak RNU6 kullanılarak -∆Ct değerleri hesaplandı. Tüm istatistiksel hesaplamalarda -∆Ct değerleri kullanıldı.
Bulgular: Benign prostat hiperplazisine kıyasla kronik prostatit ve kanser gruplarında miR-21 serum seviyelerinin upregüle olduğu ve gruplar arasındaki farklılığın istatistiksel olarak anlamlı olduğu görülmüştür (sırasıyla p=0.021 ve p=0.001). miR-21’in tek başına spesifisite ve sensitivite değerleri benign prostat hiperplazisi ile prostat kanseri ayrımında %56 ve %86 olarak tespit edilmiştir. miR-21’in miR-34a ile kombinasyonunun spesifisite ve sensitivite değerleri benign prostat hiperplazisi ile prostat kanseri ayrımında %84 ve %71 olarak hesaplanmıştır.
Sonuç: Bu çalışma ile miR-21 ve miR-21/miR-34a kombinasyonunun prostat kanseri tanısında biyobelirteç adayı olabilecek tanısal perfomansa sahip olduğu gösterilmiştir. Ayrıca, miR-21 seviyelerinde benign prostat hiperplazisine kıyasla kronik prostatit ve prostat kanserinde kademeli bir yüksekliğin olması, moleküler düzeyde gerçekleşen inflamasyon ve kanser dönüşümü süreçlerinin dolaşımdaki mikroRNA profiline de yansıdığını düşündürmektedir.

Keywords

• Prostat kanseri
• Prostatit
• Benign prostat hiperplazisi
• mikroRNA
• Tanısal etkinlik

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