EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS

Rıdvan Lawal; Gabriel Oludare; Ajibare A.c.; Brown-osawe O.o.; Odoffın M.a.; Onyemaka E.

doi:10.55549/zbs.1166109

EN

EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS

Abstract

Diethylnitrosamine (DEN) is a common rat model used in the study of the mechanism of chemical carcinogenesis and the response of Hepatocellular carcinoma (HCC) to anticancer drug therapy. The present study investigated the effect of the administration of Spondias mombin ethanolic stem-bark extract on tumor marker, cytokine mediators, protein, and albumin levels in DEN-induced liver cancer in male Wistar rats. Thirty male Wistar rats were randomly divided into six groups. Group 1 served as the normal control while Group 2 served as the negative control. Groups 3, 4, and 5 were orally treated with 200mg/kg body weight, 400 mg/kg body weight, and 800 mg/kg body weight of the extract daily respectively after induction with DEN. Group 6 served as the positive control. The level of Serum alpha-fetoprotein (AFP), activities of Interleukin-6 and Interleukin-10, protein, and albumin levels were determined using biochemical assays. From the results obtained, IL-10 activities were reduced for all treated rats when compared with negative control while rats treated with 800mg/kg showed a non-significant reduction in IL-6 activities compared with negative control. Total protein (rats treated with 800mg/kg body weight of extract) and albumin (rats treated with 400 and 800mg/kg body weight of extracts) levels were also increased non-significantly when treated with the extract after DEN administration compared to the negative control. Serum AFP which increased after DEN administration in animals was reduced except in rats treated with 800m/kg body weights of extract which showed a slight increase. The results show the therapeutic effect of Spondias mombin extract against induced hepato-carcinogenesis.

Keywords

Hepatocarcinoma, Spondias mombin, Diethylnitrosamine, serum alpha-fetoprotein, Interleukin-6, Interleukin-10, protein, albumin

References

Abe, R., Okano, J.I., Imamoto, R., Fujise, Y., Murawaki, Y. (2012). Sequential analysis of diethylnitrosamine-induced hepatocarcinogenesis in rats. Experimental and therapeutic medicine, 3(3),371–378. https://doi.org/10.3892/etm.2011.419.
Ademola I.O., Fagbemi, B.O., Idowu, S.O. (2005). Anthelmintic activity of extract of Spondias mombin against gastrointestinal nematodes of sheepstudies in vitro and in vivo. Tropical Animal Health and Production,37 (3),223 – 235.
Aggarwal, V., Tuli, H.S., Varol, A., Thakral, F., Yerer, M.B., Sak, K., Varol, M., Jain, A., Khan, M.A., Sethi, G. (2019). Role of Reactive Oxygen Species in Cancer Progression. Molecular Mechanisms and Recent Advancements. Biomolecules,9(11),735. https://doi.org/10.3390/biom9110735.
Alabi, M.A., Muthusamy, A., Kabekkodu, S., Adebawo, O., Satyamoorthy, K. (2020). Anticancer properties of recipes derived from Nigeria and African medicinal plants on breast cancer cells in vitro. https://doi.org/10.1016/j.sciaf.2020.e00446.
Ali, F.U., Mathias, C.O., Ogbanshi, M.E., Eze, U.S. (2014). Phytochemical Analysis of Spondias mombin. International Journal of Innovative Research and Development,3(9),101-107.
Ayoka, A.O., Akomolafe, R.O., Akinsomisoye, O.S., Ukponmwan, O.E. (2008). Medicinal and economic value of spondias mombin. African Journal of Biomedical Research, 11.
Budhu, A., Wang, X.W. (2006). The role of cytokines in hepatocellular carcinoma. Journal of leukocyte biology, 80(6), 1197–1213. https://doi.org/10.1189/jlb.0506297
Cabral, B., Siqueira, E.M.S., Bitencourt, M.A.O., Lima, Maíra C.J.S., Lima, A.K., Ortmann, C.F., Chaves, V.C., Fernandes-Pedrosa, M.F., Rocha, H.A.O., Scortecci, K.C., Reginatto, F.H., Giordani, R.B., Zucolotto, S.M. (2016). Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves. Rev. Bras. Farmacogn, 26, 304–311. https://doi.org/10.1016/j.bjp.2016.02.002
Féher, J., Lengyel, G. (2012). Silymarin in the prevention and treatment of liver diseases and primary liver cancer. Current pharmaceutical biotechnology, 13(1), 210–217. https://doi.org/10.2174/138920112798868818.
Globocan (2020). International Agency for Research on Cancer. World Health Organization (WHO).

Global Burden of Disease Liver Cancer Collaboration, Akinyemiju, T., Abera, S., Ahmed, M., Alam, N., Alemayohu, M.A., Allen, C., Al-Raddadi, R., Alvis-Guzman, N., Amoako, Y., Artaman, A., Ayele, T.A., Barac, A., Bensenor, I., Berhane, A., Bhutta, Z., Castillo-Rivas, J., Chitheer, A., Choi, J.Y., Cowie, B., … Fitzmaurice, C. (2017). The Burden of Primary Liver Cancer and Underlying Etiologies From 1990 to 2015 at the Global, Regional, and National Level: Results From the Global Burden of Disease Study 2015. JAMA Oncology, 3(12), 1683–1691. https://doi.org/10.1001/jamaoncol.2017.3055
Gonall, A.G., Bardawill, C.J., David, M.M. (1949). Determination of total protein. J. Biol. Chem,177, 751-760.
Hammerich, L., Tacke, F. (2014). Interleukins in chronic liver disease: lessons learned from experimental mouse models. Clinical and experimental gastroenterology, 7, 297–306. https://doi.org/10.2147/CEG.S43737
Hassan, I., Ebaid, H., Alhazza, I.M., Al-Tamimi, J., Aman, S., Abdel-Mageed, A.M. (2019). Copper Mediates Anti-Inflammatory and Antifibrotic Activity of Gleevec in Hepatocellular Carcinoma-Induced Male Rats. Canadian journal of gastroenterology & hepatology, 9897315. https://doi.org/10.1155/2019/9897315
Ibegbu, M.D., Ikekpeazu, J.E. Ezeagu, I.E, Onyekwelu, K.C, Olusanya, T.O.B. (2018). In Vivo Evaluation of Methanol and Ethanol Leaf Extracts of Spondias mombin. International Journal of Science and Research Methodology. 9(1).
Igwe, C.U., Onyeze, G.O.C., Onwuliri, V.A., Osuagwu, C.G., Ojiako, A.O. (2010). Evaluation of the Chemical Compositions of the Leaf of Spondias Mombin Linn from Nigeria. Australian Journal of Basic and Applied Sciences, 4(5), 706-710.
Iweala, E.E.J., Evbakhavbokun, W.O., Maduagwu, E.N. (2019). Antioxidant and Hepatoprotective Effect of Cajanus cajan in N-Nitrosodiethylamine-Induced Liver Damage. Scientia Pharmaceutica, 87(3), 24. https://doi.org/10.3390/scipharm87030024.
Iyer, S.S., Cheng, G. (2012). Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease. Critical reviews in immunology, 32(1), 23–63. https://doi.org/10.1615/critrevimmunol.v32.i1.30
Jahan, M.S., Vani, G., Shyamaladevi, C.S. (2011). Anti-carcinogenic Effect of Solanum trilobatum in Diethylnitrosamine Induced and Phenobarbital Promoted Heaptocarcinogenesis in Rats. Asian Journal of Biochemistry, 6, 74-81. DOI: 10.3923/ajb.2011.74.81.
Metibemu, D.S., Akinloye, O.A., Akamo, A.J., Okoye, J.O., Ojo, D.A., Morifi, E., Omotuyi, I. O. (2020). Carotenoid isolates of Spondias mombin demonstrate anticancer effects in DMBA-induced breast cancer in Wistar rats through X-linked inhibitor of apoptosis protein (XIAP) antagonism and anti-inflammation. Journal of Food Biochemistry,44(12), 13523. DOI: 10.1111/jfbc.13523.
National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. (2011). Guide for the Care and Use of Laboratory Animals. (8th ed.). National Academies Press (US).
Nelson, Z.V. (2020, July 22). Spondias mombin L. bark [Photograph]. Spondias Mombin L. Bark. https://identify.plantnet.org/observation/central-america/1007971222.
Nermin, A.H.S., Shohda, A.El-M., Manal, F.I. (2008). Diethylnitrosamine-induced hepatocarcinogenesis in rats: possible chemoprevention by blueberries. African Journal of Biochemistry Research,2, 81-87.
Nwidu, L.L., Elmorsy, E., Oboma, Y.I., Carter, W.G. (2018). Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity. Journal of Taibah University Medical Sciences, 13(3), 262–271. https://doi.org/10.1016/j.jtumed.2018.03.006.
Omoruyi, S.I., Joan, M.I., Bijou, E.A., Irobodu, M.O. (2014). Uvaria afzelii root extract protects the liver against damage caused by carbontetrachloride ingestion. J Exp Clin Anat, 13, 40-4.
Osuntokun, O., Oluduro, A.O., Omotuyi, A.O., Idowu, T. (2017). Assessment of Nephrotoxicity, Anti-Inflammatory and Antioxidant Properties of Epigallocatechin, Epicatechin and Stigmasterol Phytosterol (Synergy) Derived from Ethyl Acetate Stem Bark Extract of Spondias Mombin on Wistar Rats Using Molecular Method of Analysis. Molecular Microbiology, 1, 1-11.
Ramakrishnan, G., Raghavendran, H.R., Vinodhkumar, R., Devaki, T. (2006). Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem. Biol. Interac, 161, 104–114. doi: 10.1016/j.cbi.2006.03.007.
Rico Montanari, N., Anugwom, C.M., Boonstra, A., Debes, J.D. (2021). The Role of Cytokines in the Different Stages of Hepatocellular Carcinoma. Cancers, 13(19), 4876. https://doi.org/10.3390/cancers13194876 Sameh, S., Al-Sayed, E., Labib, R.M., Singab, A.N. (2018). Genus Spondias: A Phytochemical and Pharmacological Review. Evidence-based complementary and alternative medicine: eCAM,5382904. https://doi.org/10.1155/2018/5382904
Shewamene, Z., Dune, T., Smith, C.A. (2020). Use of traditional and complementary medicine for maternal health and wellbeing by African migrant women in Australia: a mixed method study. BMC Complement Med Ther,20(60). https://doi.org/10.1186/s12906-020-2852-6.
Tanaka, T., Kojima, T., Kawamori, T., Yoshimi, N.,Mori, H. (1993). Chemoprevention of diethylnitrosamine-induced hepatocarcinogenesis by a simple phenolic acid protocatechuic acid in rats. Cancer research, 53(12), 2775–2779.
Tolba, R., Kraus, T., Liedtke, C., Schwarz, M., Weiskirchen, R. (2015). Diethylnitrosamine (DEN)-induced carcinogenic liver injury in mice. Laboratory animals, 49(1), 59–69. https://doi.org/10.1177/0023677215570086.
Zacharakis, G., Aleid, A., Aldossari, K.K. (2018). New and old biomarkers of hepatocellular carcinoma. Hepatoma Res, 4(65). http://dx.doi.org/10.20517/2394-5079.2018.76.

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Rıdvan Lawal ^*
Nigeria

Gabriel Oludare This is me
Nigeria

Ajibare A.c. This is me
Nigeria

Brown-osawe O.o. This is me
Nigeria

Odoffın M.a. This is me
Nigeria

Onyemaka E. This is me
Nigeria

Publication Date

August 24, 2022

Submission Date

August 8, 2022

Acceptance Date

August 23, 2022

Published in Issue

Year 2022 Volume: 3 Number: 3

DOI

https://doi.org/10.55549/zbs.1166109

IZ

https://izlik.org/JA68RS94DS

APA

Lawal, R., Oludare, G., A.c., A., O.o., B.- osawe, M.a., O., & E., O. (2022). EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS. Zeugma Biological Science, 3(3), 10-19. https://doi.org/10.55549/zbs.1166109

AMA

1.Lawal R, Oludare G, A.c. A, O.o. B osawe, M.a. O, E. O. EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS. zbs. 2022;3(3):10-19. doi:10.55549/zbs.1166109

Chicago

Lawal, Rıdvan, Gabriel Oludare, Ajibare A.c., Brown-osawe O.o., Odoffın M.a., and Onyemaka E. 2022. “EFFECT OF Spondias Mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS”. Zeugma Biological Science 3 (3): 10-19. https://doi.org/10.55549/zbs.1166109.

EndNote

Lawal R, Oludare G, A.c. A, O.o. B- osawe, M.a. O, E. O (August 1, 2022) EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS. Zeugma Biological Science 3 3 10–19.

IEEE

[1]R. Lawal, G. Oludare, A. A.c., B.- osawe O.o., O. M.a., and O. E., “EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS”, zbs, vol. 3, no. 3, pp. 10–19, Aug. 2022, doi: 10.55549/zbs.1166109.

ISNAD

Lawal, Rıdvan - Oludare, Gabriel - A.c., Ajibare - O.o., Brown-osawe - M.a., Odoffın - E., Onyemaka. “EFFECT OF Spondias Mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS”. Zeugma Biological Science 3/3 (August 1, 2022): 10-19. https://doi.org/10.55549/zbs.1166109.

JAMA

1.Lawal R, Oludare G, A.c. A, O.o. B- osawe, M.a. O, E. O. EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS. zbs. 2022;3:10–19.

MLA

Lawal, Rıdvan, et al. “EFFECT OF Spondias Mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS”. Zeugma Biological Science, vol. 3, no. 3, Aug. 2022, pp. 10-19, doi:10.55549/zbs.1166109.

Vancouver

1.Rıdvan Lawal, Gabriel Oludare, Ajibare A.c., Brown-osawe O.o., Odoffın M.a., Onyemaka E. EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS. zbs. 2022 Aug. 1;3(3):10-9. doi:10.55549/zbs.1166109

Abstract

EFFECT OF Spondias mombin L. ON THE TUMOR MARKER, IMMUNOLOGICAL FUNCTION AND PROTEIN IN DIETHYLNITROSAMINE-INDUCED LIVER CANCER IN MALE WISTAR RATS

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite