Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling

Olasunkanmi Awote; Ayomide Abass

doi:10.55549/zbs.1638235

Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling

Abstract

Diabetes mellitus is still a major public health problem that comes with a lot of complications in both young and old individuals. Musa paradisiaca is abundant in the tropical and sub-tropical regions of Africa and has several medicinal traditional claims. Hence, this study evaluated the effect of Musa paradisiaca aqueous pulp extract (MPAPE) in STZ-induced diabetic rats. Bioactive compounds of MPAPE were identified and quantified using gas chromatography-mass spectroscopy (GCMS) and high-performance liquid chromatography (HPLC) techniques. A total of 24 male Wistar rats were divided into 4 groups (n=6), including control, diabetic-untreated, and diabetic-treated groups. Oxidative stress (hydrogen peroxide [H2O2], nitric oxide [NO], malondialdehyde [MDA], glutathione [GSH]); lipid profile (total cholesterol [TC], triglyceride [TRIG], high-density [HDL-Chol, low-density [LDL-Chol], and very-low-density [VLDL-Chol] lipoprotein cholesterol; liver antioxidant (superoxide dismutase [SOD], catalase [CAT]); and liver function (alanine [ALT], and aspartate [AST] aminotransferase) enzymes were evaluated using standard biochemical kits and procedures. PyRx and Biovia Discovery Studio were used for molecular docking studies while swissADME and ProTox were used to predict the ADME/T properties. The results of the phytochemistry of M. paradisiaca pulp showed the presence of alkaloids, tannins, flavonoids, saponins, and phenols, an abundant polyphenolic content. The administration of MPAPE (300 mg/kg body weight) significantly (p<0.05) decreased the high levels of glucose, total-chol, LDL-chol, TRIG, VLDL-chol, H2O2, NO, MDA, and the activities of SOD and CAT while, decreasing ALT and AST activities, and HDL-Chol, and GSH levels. In conclusion, MPAPE may possess antioxidant and antidiabetic potentials in the management of diabetes.

Keywords

Traditional medicine, Medicinal plants, Nutraceuticals, Musa paradisiaca fruit, Oxidative stress, Diabetes, Liver damage, Antioxidant, Streptozotocin, Phytochemistry.

Project Number

2

Ethical Statement

The current national and international criteria were followed in the care and usage of the Wistar rats during the study. With the ethical approval number LASU/23/REC/055, the Lagos State University Research Ethics Committee authorized the study procedure.

Thanks

The authors appreciate the mercies, grace, favour, and help of God Almighty for the successful completion of this research.

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Review Article

Authors

Olasunkanmi Awote ^*
0000-0002-0727-3998
Nigeria

Ayomide Abass
0009-0006-1120-5257
Nigeria

Early Pub Date

March 29, 2025

Publication Date

April 2, 2025

Submission Date

February 12, 2025

Acceptance Date

February 26, 2025

Published in Issue

Year 2025 Volume: 6 Number: 2

DOI

https://doi.org/10.55549/zbs.1638235

IZ

https://izlik.org/JA55PN52SH

APA

Awote, O., & Abass, A. (2025). Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling. Zeugma Biological Science, 6(2), 16-41. https://doi.org/10.55549/zbs.1638235

AMA

1.Awote O, Abass A. Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling. zbs. 2025;6(2):16-41. doi:10.55549/zbs.1638235

Chicago

Awote, Olasunkanmi, and Ayomide Abass. 2025. “Evaluation of Unripe Musa Paradisiaca Pulp Extract in Streptozotocin-Induced Type-2 Diabetic Rats: In-Vivo Studies and In-Silico Modelling”. Zeugma Biological Science 6 (2): 16-41. https://doi.org/10.55549/zbs.1638235.

EndNote

Awote O, Abass A (April 1, 2025) Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling. Zeugma Biological Science 6 2 16–41.

IEEE

[1]O. Awote and A. Abass, “Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling”, zbs, vol. 6, no. 2, pp. 16–41, Apr. 2025, doi: 10.55549/zbs.1638235.

ISNAD

Awote, Olasunkanmi - Abass, Ayomide. “Evaluation of Unripe Musa Paradisiaca Pulp Extract in Streptozotocin-Induced Type-2 Diabetic Rats: In-Vivo Studies and In-Silico Modelling”. Zeugma Biological Science 6/2 (April 1, 2025): 16-41. https://doi.org/10.55549/zbs.1638235.

JAMA

1.Awote O, Abass A. Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling. zbs. 2025;6:16–41.

MLA

Awote, Olasunkanmi, and Ayomide Abass. “Evaluation of Unripe Musa Paradisiaca Pulp Extract in Streptozotocin-Induced Type-2 Diabetic Rats: In-Vivo Studies and In-Silico Modelling”. Zeugma Biological Science, vol. 6, no. 2, Apr. 2025, pp. 16-41, doi:10.55549/zbs.1638235.

Vancouver

1.Olasunkanmi Awote, Ayomide Abass. Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling. zbs. 2025 Apr. 1;6(2):16-41. doi:10.55549/zbs.1638235

Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling

Abstract

Keywords

Project Number

Ethical Statement

Thanks

References

Details

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Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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