Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Bushra Parveen; Yasmeen Junejo; Muhammad Safdar; Mehmet Özaslan

EN

Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Abstract

The present study was structured for molecular detection and characterization of Escherichia. coli (E. coli) isolated from cow’s raw milk samples. A total of 100 milk samples were collected in a way that 10 samples each were collected from markets of Jamalpur, Hasilpur, Khairpur, Qaimpur, Lal Sohanra, Lal Sohanra Park, Yazman Mandi, Rajkan, Ahmedpur East and Uch Sharif, district Bahawalpur, Pakistan. Milk samples were then cultured on different culture media for bacterial isolation. Identification of bacteria was done through Gram’s staining, properties of bacterial culture on different selective media and biochemical tests including catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Out of 100 samples, 43 samples were found to be contaminated with E. coli. E. coli isolates were then amplified by 16S rRNA gene-based PCR. Antimicrobial sensitivity test was also performed to confirm the susceptibility of E. coli to different antibiotics. Results of the antimicrobial sensitivity test showed that the E. coli isolates were resistant to amoxicillin and erythromycin but sensitive to azithromycin, ciprofloxacin, gentamicin, norfloxacin and streptomycin.

Keywords

E. coli, PCR, Cow Milk, Antimicrobial activity

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Details

Primary Language

English

Subjects

Food Engineering

Journal Section

Research Article

Authors

Bushra Parveen This is me
Pakistan

Yasmeen Junejo This is me
Pakistan

Muhammad Safdar ^*
Pakistan

Mehmet Özaslan
Türkiye

Publication Date

April 26, 2021

Submission Date

April 11, 2021

Acceptance Date

April 26, 2021

Published in Issue

Year 2021 Volume: 2 Number: 2

IZ

https://izlik.org/JA23JR59DH

APA

Parveen, B., Junejo, Y., Safdar, M., & Özaslan, M. (2021). Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. Zeugma Biological Science, 2(2), 1-13. https://izlik.org/JA23JR59DH

AMA

1.Parveen B, Junejo Y, Safdar M, Özaslan M. Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. zbs. 2021;2(2):1-13. https://izlik.org/JA23JR59DH

Chicago

Parveen, Bushra, Yasmeen Junejo, Muhammad Safdar, and Mehmet Özaslan. 2021. “Molecular Characterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from District Bahawalpur, Pakistan”. Zeugma Biological Science 2 (2): 1-13. https://izlik.org/JA23JR59DH.

EndNote

Parveen B, Junejo Y, Safdar M, Özaslan M (April 1, 2021) Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. Zeugma Biological Science 2 2 1–13.

IEEE

[1]B. Parveen, Y. Junejo, M. Safdar, and M. Özaslan, “Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan”, zbs, vol. 2, no. 2, pp. 1–13, Apr. 2021, [Online]. Available: https://izlik.org/JA23JR59DH

ISNAD

Parveen, Bushra - Junejo, Yasmeen - Safdar, Muhammad - Özaslan, Mehmet. “Molecular Characterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from District Bahawalpur, Pakistan”. Zeugma Biological Science 2/2 (April 1, 2021): 1-13. https://izlik.org/JA23JR59DH.

JAMA

1.Parveen B, Junejo Y, Safdar M, Özaslan M. Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. zbs. 2021;2:1–13.

MLA

Parveen, Bushra, et al. “Molecular Characterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from District Bahawalpur, Pakistan”. Zeugma Biological Science, vol. 2, no. 2, Apr. 2021, pp. 1-13, https://izlik.org/JA23JR59DH.

Vancouver

1.Bushra Parveen, Yasmeen Junejo, Muhammad Safdar, Mehmet Özaslan. Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. zbs [Internet]. 2021 Apr. 1;2(2):1-13. Available from: https://izlik.org/JA23JR59DH

Abstract

Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

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