Evaluation of Unripe Musa paradisiaca Pulp Extract in Streptozotocin-induced Type-2 Diabetic Rats: In-vivo Studies and In-silico Modelling

Year 2025, Volume: 6 Issue: 2, 16 - 41, 02.04.2025

Olasunkanmi Awote , Ayomide Abass

https://doi.org/10.55549/zbs.1638235

Abstract

Diabetes mellitus is still a major public health problem that comes with a lot of complications in both young and old individuals. Musa paradisiaca is abundant in the tropical and sub-tropical regions of Africa and has several medicinal traditional claims. Hence, this study evaluated the effect of Musa paradisiaca aqueous pulp extract (MPAPE) in STZ-induced diabetic rats. Bioactive compounds of MPAPE were identified and quantified using gas chromatography-mass spectroscopy (GCMS) and high-performance liquid chromatography (HPLC) techniques. A total of 24 male Wistar rats were divided into 4 groups (n=6), including control, diabetic-untreated, and diabetic-treated groups. Oxidative stress (hydrogen peroxide [H2O2], nitric oxide [NO], malondialdehyde [MDA], glutathione [GSH]); lipid profile (total cholesterol [TC], triglyceride [TRIG], high-density [HDL-Chol, low-density [LDL-Chol], and very-low-density [VLDL-Chol] lipoprotein cholesterol; liver antioxidant (superoxide dismutase [SOD], catalase [CAT]); and liver function (alanine [ALT], and aspartate [AST] aminotransferase) enzymes were evaluated using standard biochemical kits and procedures. PyRx and Biovia Discovery Studio were used for molecular docking studies while swissADME and ProTox were used to predict the ADME/T properties. The results of the phytochemistry of M. paradisiaca pulp showed the presence of alkaloids, tannins, flavonoids, saponins, and phenols, an abundant polyphenolic content. The administration of MPAPE (300 mg/kg body weight) significantly (p<0.05) decreased the high levels of glucose, total-chol, LDL-chol, TRIG, VLDL-chol, H2O2, NO, MDA, and the activities of SOD and CAT while, decreasing ALT and AST activities, and HDL-Chol, and GSH levels. In conclusion, MPAPE may possess antioxidant and antidiabetic potentials in the management of diabetes.

Keywords

Traditional medicine, Medicinal plants, Nutraceuticals, Musa paradisiaca fruit, Oxidative stress, Diabetes, Liver damage, Antioxidant, Streptozotocin, Phytochemistry.

Ethical Statement

The current national and international criteria were followed in the care and usage of the Wistar rats during the study. With the ethical approval number LASU/23/REC/055, the Lagos State University Research Ethics Committee authorized the study procedure.

Project Number

2

Thanks

The authors appreciate the mercies, grace, favour, and help of God Almighty for the successful completion of this research.

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