Araştırma Makalesi
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Bazı Yerel Entomopatojen Fungusların Toprak Koşullarındaki Etkinliklerinin Tenebrio molitor L. (Col.: Tenebrionidae) Larvaları Kullanılarak Araştırılması

Yıl 2019, Cilt: 2 Sayı: 1, 26 - 31, 02.05.2019

Öz

Birçok entomopatojen fungus türü ticari
olarak üretilmekte ve zararlı böceklerin mücadelesinde başarıyla
kullanılmaktadır. Yapılan bu çalışmada, Düzce ilinden elde edilen bazı yerel Beauveria bassiana ve Metarhizium anisopliae izolatları
kullanılmış ve bu izolatların toprak koşullarındaki etkinlikleri un kurdu, Tenebrio molitor larvaları kullanılarak araştırılmıştır.
İzolatlar çoğunlukla ormanlık ve tarım alanlarından elde edilmiştir. Toplam 12
izolat toprağa 1x107 konidi ml-1 yoğunlukta
uygulanmıştır. Uygulama sonrası topraklar kavanozlara alınmış ve her bir
kavanoza 10 adet T. molitor larvası
konularak 7 gün süreyle takip edilmiştir. Her bir izolat ve kontrol grubu için
denemler üç tekrar halinde gerçekleştirilmiştir. İzolatların toprak
koşullarında T. molitor larvalarına
karşı oldukça etkili oldukları görülmüştür. B.
bassiana
YK14, M. anisopliae
YK38 ve YK45 izolatları T. molitor
larvalarının tamamını 7 gün içerisinde öldürmüştür. Diğer izolatların etkinlikleri
ise %20 ile %93.33 arasında değişiklik göstermiştir. Etkili yerel B. bassiana ve M. anisopliae
izolatlarının toprak altı zararlılarla mücadelede kullanılabileceğine yönelik
bulgular ortaya çıkmıştır.

Destekleyen Kurum

Düzce Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2017.11.02.651

Teşekkür

Bu çalışma Düzce Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (Proje no: 2017.11.02.651). Yazarlar, böcek kültürü temini için Dr. Öğr. Üyesi Barış Gülcü’ye ayrıca teşekkür etmektedir.

Kaynakça

  • Arthur, F.H., 1996. Grain protectants: current status and prospects for the future. Journal of Stored Products Research, 32: 293-302.
  • Ayvaz, A., Albayrak, S., Karaborklu, S., 2008. Gamma radiation sensitivity of the eggs, larvae and pupae of Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Pest Management Science: 64, 505–512.
  • Azizoglu, U., Yilmaz, S., Karaborklu, S., Ayvaz A., 2011. Ovicidal activity of microwave and UV radiations on mediterranean flour moth Ephestia kuehniella Zeller, 1879 ( Lepidoptera: Pyralidae). Turkiye Entomoloji Dergisi-Turkish Journal of Entomology, 35: 437-446.
  • Batta, Y., Murdoch, G., Mansfield, S., 2010. Investigations into the formulation and application of entomopathogenic fungi against larvae of yellow meal worm (Tenebrio molitor L., Coleoptera: Tenebrionidae). General and Applied Entomology, 39: 5–8.
  • Goettel, M.S., Eilenberg, J., Glare, T., 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: L.I. Gilbert, K. Iatrou, S.S. Gill, (Eds), Comprehensive Molecular Insect Science, Elseiver, Amsterdam, pp. 361-405.
  • Jackson, M.A., Jaronski, S.T., 2009.Production of microsclerotia of the fungal entomopathogen Metarhizium anisopliae and their potential for use as a biocontrol agent for soil-inhabiting insects. Mycological Research, 113: 842-850.
  • Karabörklü, S., Azizoglu, U., Azizoglu, Z.B., 2018. Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control. World Journal of Microbiology and Biotechnology, 34, 14.
  • Lacey, L.A., Goettel M.S., 1995. Current developments in microbial control of insect pests and prospects for the early 21st century. Entomophaga, 40: 3-27.
  • Moore, D. Prior C., 1993. The potential of mycoinsecticides. Biocontrol News and Information, 14: 31-40.
  • Quesada-Moraga, E., Navas-Cortes, J.A., Maranhao, E.A.A., Ortiz-Urquiza, A., Santiago-Alvarez, C., 2007. Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research, 111: 947-966.
  • Rath, A.C., 2000. The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10: 563-581.
  • Rice, W.C., Cogburn, R.R., 1999. Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three coleopteran pests of stored grain. Journal of Economic Entomology, 92: 691–694.
  • Rohde, C., Alves, L., Neves, P., Alves, S.B., Da Silva, E., De Almeida J., 2006. Seleção de isolados de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. contra o Cascudinho Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotrophical Entomology, 35(2): 231–240.
  • Sammaritano, J.A.A., Leclerque, A., Deymié, M.C., Torrente, K.A., Vazquez, F., Cuthbertson, A.G., Lastra, C.C.L., Lechner, B., 2017. Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains? Revista de la Sociedad Entomológica Argentina, 76 (3-4): 39-43.
  • Sanchez-Pena, S.R., Lara, J.S.J., Medina, R.F., 2011. Occurrence of entomopathogenic fungi from agricultural and natural ecosystems in Saltillo, Mexico, and their virulence towards thrips and whiteflies. Journal of Insect Science, 11, 10.
  • Schöller, M., Prozell, S., Al-Kirshi, A.G., Reichmuth, C., 1997. Towards biological control as a major component of integrated pest management in stored product protection. Journal of Stored Products Research, 33 (1): 81-97.
  • Sevim, A., Sevim, E., Demirbağ, Z., 2015. Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(1): 115-147.
  • Shah, P.A., Pell, J.K., 2003. Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology, 61: 413–423.
  • Zettler, J.L., Arthur, F.H., 2000. Chemical control of stored product insects with fumigants and residual treatments. Crop Protection, 19: 577–582.

Efficiency of Some Native Entomopathogenic Fungi against Tenebrio molitor L. (Col.: Tenebrionidae) Larvae in Soil Condition

Yıl 2019, Cilt: 2 Sayı: 1, 26 - 31, 02.05.2019

Öz

Many entomopathogenic fungi have been commercially produced and successfully used to control pest insects. In this study, some Beauveria bassiana and Metarhizium anisopliae isolates were obtained from Duzce province and the efficiency of these isolates investigated against the larvae of mealworm beetle, Tenebrio molitor in soil condition. Isolates are mostly obtained from forest and agricultural fields. A total of 12 isolates were applied to the soil at 1x107 conidia ml-1 concentration. After the application, the soil was taken into the jars and 10 T. molitor larvae were added for 7 days. Trials were performed as three replicates for each isolate and control group. It has been found that isolates are highly effective against T. molitor larvae. B. bassiana YK14, M. anisopliae YK38 and YK45 isolates caused 100% mortality against T. molitor larvae after the application of 7 days. The efficiency of other isolates varied between 20% and 93.33%. Results showed that the effective native B. bassiana and M. anisopliae isolates can be used for the control of soil-inhabiting pest insects.

Proje Numarası

2017.11.02.651

Kaynakça

  • Arthur, F.H., 1996. Grain protectants: current status and prospects for the future. Journal of Stored Products Research, 32: 293-302.
  • Ayvaz, A., Albayrak, S., Karaborklu, S., 2008. Gamma radiation sensitivity of the eggs, larvae and pupae of Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Pest Management Science: 64, 505–512.
  • Azizoglu, U., Yilmaz, S., Karaborklu, S., Ayvaz A., 2011. Ovicidal activity of microwave and UV radiations on mediterranean flour moth Ephestia kuehniella Zeller, 1879 ( Lepidoptera: Pyralidae). Turkiye Entomoloji Dergisi-Turkish Journal of Entomology, 35: 437-446.
  • Batta, Y., Murdoch, G., Mansfield, S., 2010. Investigations into the formulation and application of entomopathogenic fungi against larvae of yellow meal worm (Tenebrio molitor L., Coleoptera: Tenebrionidae). General and Applied Entomology, 39: 5–8.
  • Goettel, M.S., Eilenberg, J., Glare, T., 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: L.I. Gilbert, K. Iatrou, S.S. Gill, (Eds), Comprehensive Molecular Insect Science, Elseiver, Amsterdam, pp. 361-405.
  • Jackson, M.A., Jaronski, S.T., 2009.Production of microsclerotia of the fungal entomopathogen Metarhizium anisopliae and their potential for use as a biocontrol agent for soil-inhabiting insects. Mycological Research, 113: 842-850.
  • Karabörklü, S., Azizoglu, U., Azizoglu, Z.B., 2018. Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control. World Journal of Microbiology and Biotechnology, 34, 14.
  • Lacey, L.A., Goettel M.S., 1995. Current developments in microbial control of insect pests and prospects for the early 21st century. Entomophaga, 40: 3-27.
  • Moore, D. Prior C., 1993. The potential of mycoinsecticides. Biocontrol News and Information, 14: 31-40.
  • Quesada-Moraga, E., Navas-Cortes, J.A., Maranhao, E.A.A., Ortiz-Urquiza, A., Santiago-Alvarez, C., 2007. Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research, 111: 947-966.
  • Rath, A.C., 2000. The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10: 563-581.
  • Rice, W.C., Cogburn, R.R., 1999. Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three coleopteran pests of stored grain. Journal of Economic Entomology, 92: 691–694.
  • Rohde, C., Alves, L., Neves, P., Alves, S.B., Da Silva, E., De Almeida J., 2006. Seleção de isolados de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. contra o Cascudinho Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotrophical Entomology, 35(2): 231–240.
  • Sammaritano, J.A.A., Leclerque, A., Deymié, M.C., Torrente, K.A., Vazquez, F., Cuthbertson, A.G., Lastra, C.C.L., Lechner, B., 2017. Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains? Revista de la Sociedad Entomológica Argentina, 76 (3-4): 39-43.
  • Sanchez-Pena, S.R., Lara, J.S.J., Medina, R.F., 2011. Occurrence of entomopathogenic fungi from agricultural and natural ecosystems in Saltillo, Mexico, and their virulence towards thrips and whiteflies. Journal of Insect Science, 11, 10.
  • Schöller, M., Prozell, S., Al-Kirshi, A.G., Reichmuth, C., 1997. Towards biological control as a major component of integrated pest management in stored product protection. Journal of Stored Products Research, 33 (1): 81-97.
  • Sevim, A., Sevim, E., Demirbağ, Z., 2015. Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(1): 115-147.
  • Shah, P.A., Pell, J.K., 2003. Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology, 61: 413–423.
  • Zettler, J.L., Arthur, F.H., 2000. Chemical control of stored product insects with fumigants and residual treatments. Crop Protection, 19: 577–582.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Çevre Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Yeşim Kesin Bu kişi benim

Salih Karabörklü

Nedim Altın

Proje Numarası 2017.11.02.651
Yayımlanma Tarihi 2 Mayıs 2019
Gönderilme Tarihi 5 Haziran 2018
Yayımlandığı Sayı Yıl 2019 Cilt: 2 Sayı: 1

Kaynak Göster

Vancouver Kesin Y, Karabörklü S, Altın N. Bazı Yerel Entomopatojen Fungusların Toprak Koşullarındaki Etkinliklerinin Tenebrio molitor L. (Col.: Tenebrionidae) Larvaları Kullanılarak Araştırılması. TUBİD. 2019;2(1):26-31.