Tescilli Fasulye Çeşitlerinin Pas (Uromyces appendiculatus) Etmenine Karşı Dayanıklılık Durumlarının SCAR Markörleri ile Belirlenmesi

Yıl 2019, Cilt: 6 Sayı: 3, 410 - 416, 23.07.2019

Mehmet Zahit Yeken Göksel Özer Ali Çelik Vahdettin Çiftçi

https://doi.org/10.30910/turkjans.595202

Cited By: 1

Öz

Fasulye pas
hastalığı tüm dünyada fasulye üretiminde sorun oluşturan önemli fungal
hastalıklardan birisidir.  Fasulye pası
ile en etkili ve çevre açısından en zararsız mücadele şekli dayanıklı çeşit
ıslahı ve kullanımıdır. Son yıllarda geliştirilen moleküler markörler sayesinde
hastalıklara karşı dayanıklılık önceden belirlenebilmekte ve ıslah programları
bu veriler ışığında planlanabilmektedir. Bu çalışmada, Türkiye’de yaygın olarak
yetiştiriciliği yapılan 43 tescilli fasulye çeşidinin pas hastalığına
dayanıklılık durumları SCAR markörleri ile incelenmiştir. Dayanıklılıkla ilgili
4 farklı markör (SA14, SI19, SBC6, AE19) yardımıyla yapılan PCR çalışmaları
bazı çeşitlerin pas hastalığına karşı dayanıklılık sağlayan genlere sahip olduklarını
ortaya çıkarmıştır. Elde edilen tarama sonuçlarının mevcut çeşitlerin
dayanıklılık durumlarını ortaya koymasının yanı sıra, pas ile ilgili yapılacak
moleküler destekli dayanıklılık ıslahı çalışmalarının temelini oluşturacağı ön
görülmektedir.

Anahtar Kelimeler

Pas, Uromyces appendiculatus

Teşekkür

Bu çalışma Bolu Abant İzzet Baysal Üniversitesi Bilimsel Araştırma Projeleri birimi tarafından BAP – 2016.10.07.1091 no’lu proje kapsamında desteklenmiştir.

Determination of the Resistance to the Bean Rust (Uromyces appendiculatus) in Registered Bean Varieties by Using SCAR Markers

Öz

Bean rust disease is one of the most important fungal diseases of
limiting the yields of common bean all over the world.  The most effective and the most harmless
methods against the bean rust pathogen are breeding and use of the resistant
varieties. Resistance to diseases can be previously determined in breeding
programs with the critical data obtained from molecular markers developed in
recent years. In this study, resistance against bean rust was investigated by
SCAR markers in 43 registered bean varieties, which is widely cultivated in
Turkey. As a result, PCR studies performed with 4 different markers (SA14,
SI19, SBC6, AE19) which characterize the genes related to resistance revealed
that some varieties have the genes linked to the resistance. In addition to
revealing the current status of the registered varieties, the results of the
study are expected to support new breeding programs. 

Anahtar Kelimeler

Bean rust, Uromyces appendiculatus

Kaynakça

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