Tannik asitle irrigasyonun kök kanal dentininin mikrosertliğine ve epoksi rezin esaslı patın bağlanma dayanımına etkisi
Öz
Amaç: Tannik asit (TA) içeren final irrigasyon protokollerinin
dentin miksrosertliğine ve epoksi rezin
içerikli patın kök kanal dentinine push-out bağlanma dayanımına etksinin incelenmesi
Gereç ve Yöntemler: 50 adet yarım kök mikrosertlik
ölçümü için akrilik rezine gömüldü. İşlem öncesi miksrosertlik değerleri elde
edildikten sonra örnekler kullanılacak olan final irrigasyon protokolüne göre
rastgele 5 gruba dağıtıldı (n=10): distile su (kontrol),
% 10 TA 2
dk, %10 TA 5dk, % 20 TA 2 dk, ve %20 TA 5 dk.
Dentin yüzeyleri irrigasyon solüsyonları ile muamele edildikten sonra başlangıç
ölçümlerinin yapıldığı noktalara yakın olacak şekilde mikrosertlik değerleri
tekrar ölçülüp kaydedildi.
Push-out bağlanma dayanımı testi için 75 adet tek köklü mandibular
premolar dişin kanalı genişletildi ve final irrigasyon protokolü yukarıda bahsedildiği şekilde
uygulandı (n = 15). Örneklerin kanalları tek kon yöntemi kullanılarak uyumlu
gütaperka konlar ve AH Plus- epoksi rezin içerikli patla doldurulduktan sonra
köklerin orta üçlü bölümünden 1-mm kalınlığında kesitler elde edildi. Push-out
bağlanma testi uygulandı. Veriler tek yönlü varyans analizi (ANOVA) ve Tukey
HSD testleri kullanılarak değerlendirildi (p=0.05).
Bulgular:
TA uygulanmış gruplarda mikrosertlik değerleri kontrol grubuna gore anlamlı
artış gösterirken (p<0.05), TA uygulanmış grupların mikrosertlik değerleri
kendi aralarında anlamlı bir fark göstermedi (p>0.05).
Push-out bağlanma dayanımı testi sonucunda % 10 TA 2 dak. grubu
ile kontrol grubu arasında anlamlı fark bulunamadı (p>0.05). Diğer TA
grupları kontrol grubuna gore daha yüksek bağlanma dayanımı değerleri
gösterirken (p<0.05), aralarında anlamlı fark bulunamadı (p>0.05).
Sonuç:
TA ile final irrigasyon yapılması dentin mikrosertliğini ve epoksi rezin
içerikli kanal dolgu patının kök kanal dentinine bağlanma dayanımını arttırdı.
TA’nın bağlanma dayanımı üzerindeki etkisi zaman ve konsantrasyon bağımlıdır.
Anahtar Kelimeler
dentin epoksi rezin esaslı pat bağlanma dayanımı mikrosertlik
Kaynakça
- 1. Schilder H. Filling root canals in three dimensions. Dent Clin North Am 1967;11:723-44.
- 2. Grossman LI. Physical properties of root canal cements. J Endod 1976;2:166-75.
- 3. Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature -- Part 2. Influence of clinical factors. Endod J 2008;41:6-31.
- 4. Butler WT. Dentin matrix proteins and dentinogenesis. Connect Tissue Res 1995;33: 59-65.
- 5. Al‐Ammar A, Drummond JL, Bedran‐Russo AK. The use of collagen cross‐linking agents to enhance dentin bond strength. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2009;91:419-24.
- 6. Neelakantan P, Subbarao C, Subbarao CV, De-Deus G, Zehnder M. The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. Int Endod J 2011;44: 491-8.
- 7. Kalra M, Iqbal K, Nitisusanta LI, Daood U, Sum CP, Fawzy AS. The effect of proanthocyanidins on the bond strength and durability of resin sealer to root dentine. Int Endod J 2013;46:169-78.
- 8. G Pheenithicharoenkul S, Panichuttra A. Epigallocatechin-3-gallate increased the push out bond strength of an epoxy resin sealer to root dentin. Dent Mater J 2016;35: 888-92
- 9. Bedran-Russo AK, Yoo KJ, Ema KC, Pashley DH. Mechanical properties of tannic-acid-treated dentin matrix. J Dent Res 2009;88:807-11.
- 10. Zehnder M. Root canal irrigants. J Endod 2006; 32:389-98.
- 11. Sayin TC, Serper A, Cehreli ZC, Otlu HG. The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;104:418-24.
- 12. Sim T, Knowles J, Ng YL, Shelton J, Gulabivala K. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Int Endod J 2001; 34:120-32.
- 13. Moreira DM, Almeida JFA, Ferraz CCR, de Almeida Gomes BPF, Line SRP, Zaia AA. Structural analysis of bovine root dentin after use of different endodontics auxiliary chemical substances. J Endod 2009; 35: 1023-7.
- 14. Mai S, Kim YK, Arola DD, Gu LS, Kim JR, Pashley DH, et al. Differential aggressiveness of ethylenediamine tetraacetic acid in causing canal wall erosion in the presence of sodium hypochlorite. J Dent 2010;38:201-16.
- 15. Santos JN, de Oliveira Carrilho MR, De Goes MF, Zaia AA, de Almeida Gomes BPF, de Souza-Filho FJ, et al. Effect of chemical irrigants on the bond strength of a self-etching adhesive to pulp chamber dentin. J Endod 2006;32:1088-90.
- 16. Vilanova WV, Carvalho-Junior JR, Alfredo E, Sousa-Neto MD, Silva-Sousa YT. Effect of intracanal irrigants on the bond strength of epoxy resin-based and methacrylate resin-based sealers to root canal walls. Int Endod J 2012;45:42-8.
- 17. Jastrzebska M, Zalewska-Rejdak J, Wrzalik R, Kocot A, Mroz I, Barwinski B, et al. Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. J Biomed Mater Res A 2006;78:148-56.
- 18. Serrano J, Puupponen-Pimia R, Dauer A, Aura AM, Saura-Calixto F. Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Mol Nutr Food Res 2009;53:310-29.
- 19. Bedran‐Russo AKB, Pereira PN, Duarte WR, Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007;80:268-72.
- 20. Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of Irrigation Sequence on the Adhesion of Root Canal Sealers to Dentin: A Fourier Transform Infrared Spectroscopy and Push-out Bond Strength Analysis. J Endod 2015;41: 1108-11.
- 21. Xie Q, Bedran-Russo AK, Wu CD. In vitro remineralization effects of grape seed extract on artificial root caries. J Dent 2008;36:900-6.
- 22. Tang CF, Fang M, Liu RR, Dou Q, Chai ZG, Xiao YH, et al. The role of grape seed extract in the remineralization of demineralized dentine: micromorphological and physical analyses. Arch Oral Biol 2013;58:1769-1776.
- 23. Christopher SR, Mathai V, Nair RS, Angelo JM. The effect of three different antioxidants on the dentinal tubular penetration of Resilon and Real Seal SE on sodium hypochlorite-treated root canal dentin: An in vitro study. J Conserv Dent 2016;19:161-5.
- 24. Ferk Luketic S, Malcic A, Jukic S, Anic I, Segovic S, Kalenic S. Coronal microleakage of two root-end filling materials using a polymicrobial marker. J Endod 2008; 34: 201-3.
- 25. Balguerie E, van der Sluis L, Vallaeys K, Gurgel-Georgelin M, Diemer F. Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study. J Endod 2011;37:1576-9.
- 26. Sirisha K, Rambabu T, Shankar YR, Ravikumar P. Validity of bond strength tests: A critical review: Part I. J Conserv Dent 2014;17:305-11.
- 27. Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod 2013;39:669-73.
Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer
Öz
Background:To investigate the effects
of final irrigation protocols with tannic acid (TA) on dentin microhardness and
push-out bond strength of an epoxy resin based sealer to root canal dentin.
Methods: The 50 root halves were embedded in an acrylic resin for the
microhardness measurement. After
the initial baseline microhardness measurement, the root halves were randomly
divided into 5 groups according to the final irrigation protocol used (n=10): distilled water (control), 10% TA for 2 min, 10% TA for 5 min, 20% TA for
2 min, and 20% TA for 5 min.
After surface treatment, dentin microhardness values were recorded at close
proximity to the initial indentation areas.
For push-out bond strength test the root canals of 75
single-rooted mandibular premolars were instrumented and the final irrigation protocols
were applied as described above (n = 15). Following
root canal obturation with single cone technique using matched-taper gutta percha cones and
an
AH Plus- epoxy resin based root canal sealer, 1 mm-thick slices were obtained from the
middle third of the root canals. Push-out
bond strength test was applied. The data were analyzed using One way ANOVA and
Tukey HSD tests (p=0.05).
Results:In TA-treated groups, there was a
significant increase in the microhardness values compared with the control
group (p<0.05). Regarding the push-out bond strength test, there was no significant
difference between the bond strength values of 10% TA for 2 min and the control
group (p>0.05). The remaining groups presented higher bond strength values
than the control group (p<0.05).
Conclusions: TA increased dentin microhardness and the
bond strength of the epoxy resin based sealer to root canal dentin. The effect
on the bond strength was time and concentration dependent.
Keywords: bond strength, dentin, microhardness, epoxy resin based sealer, tannic
acid
Anahtar Kelimeler
Kaynakça
- 1. Schilder H. Filling root canals in three dimensions. Dent Clin North Am 1967;11:723-44.
- 2. Grossman LI. Physical properties of root canal cements. J Endod 1976;2:166-75.
- 3. Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature -- Part 2. Influence of clinical factors. Endod J 2008;41:6-31.
- 4. Butler WT. Dentin matrix proteins and dentinogenesis. Connect Tissue Res 1995;33: 59-65.
- 5. Al‐Ammar A, Drummond JL, Bedran‐Russo AK. The use of collagen cross‐linking agents to enhance dentin bond strength. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2009;91:419-24.
- 6. Neelakantan P, Subbarao C, Subbarao CV, De-Deus G, Zehnder M. The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. Int Endod J 2011;44: 491-8.
- 7. Kalra M, Iqbal K, Nitisusanta LI, Daood U, Sum CP, Fawzy AS. The effect of proanthocyanidins on the bond strength and durability of resin sealer to root dentine. Int Endod J 2013;46:169-78.
- 8. G Pheenithicharoenkul S, Panichuttra A. Epigallocatechin-3-gallate increased the push out bond strength of an epoxy resin sealer to root dentin. Dent Mater J 2016;35: 888-92
- 9. Bedran-Russo AK, Yoo KJ, Ema KC, Pashley DH. Mechanical properties of tannic-acid-treated dentin matrix. J Dent Res 2009;88:807-11.
- 10. Zehnder M. Root canal irrigants. J Endod 2006; 32:389-98.
- 11. Sayin TC, Serper A, Cehreli ZC, Otlu HG. The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;104:418-24.
- 12. Sim T, Knowles J, Ng YL, Shelton J, Gulabivala K. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Int Endod J 2001; 34:120-32.
- 13. Moreira DM, Almeida JFA, Ferraz CCR, de Almeida Gomes BPF, Line SRP, Zaia AA. Structural analysis of bovine root dentin after use of different endodontics auxiliary chemical substances. J Endod 2009; 35: 1023-7.
- 14. Mai S, Kim YK, Arola DD, Gu LS, Kim JR, Pashley DH, et al. Differential aggressiveness of ethylenediamine tetraacetic acid in causing canal wall erosion in the presence of sodium hypochlorite. J Dent 2010;38:201-16.
- 15. Santos JN, de Oliveira Carrilho MR, De Goes MF, Zaia AA, de Almeida Gomes BPF, de Souza-Filho FJ, et al. Effect of chemical irrigants on the bond strength of a self-etching adhesive to pulp chamber dentin. J Endod 2006;32:1088-90.
- 16. Vilanova WV, Carvalho-Junior JR, Alfredo E, Sousa-Neto MD, Silva-Sousa YT. Effect of intracanal irrigants on the bond strength of epoxy resin-based and methacrylate resin-based sealers to root canal walls. Int Endod J 2012;45:42-8.
- 17. Jastrzebska M, Zalewska-Rejdak J, Wrzalik R, Kocot A, Mroz I, Barwinski B, et al. Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. J Biomed Mater Res A 2006;78:148-56.
- 18. Serrano J, Puupponen-Pimia R, Dauer A, Aura AM, Saura-Calixto F. Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Mol Nutr Food Res 2009;53:310-29.
- 19. Bedran‐Russo AKB, Pereira PN, Duarte WR, Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007;80:268-72.
- 20. Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of Irrigation Sequence on the Adhesion of Root Canal Sealers to Dentin: A Fourier Transform Infrared Spectroscopy and Push-out Bond Strength Analysis. J Endod 2015;41: 1108-11.
- 21. Xie Q, Bedran-Russo AK, Wu CD. In vitro remineralization effects of grape seed extract on artificial root caries. J Dent 2008;36:900-6.
- 22. Tang CF, Fang M, Liu RR, Dou Q, Chai ZG, Xiao YH, et al. The role of grape seed extract in the remineralization of demineralized dentine: micromorphological and physical analyses. Arch Oral Biol 2013;58:1769-1776.
- 23. Christopher SR, Mathai V, Nair RS, Angelo JM. The effect of three different antioxidants on the dentinal tubular penetration of Resilon and Real Seal SE on sodium hypochlorite-treated root canal dentin: An in vitro study. J Conserv Dent 2016;19:161-5.
- 24. Ferk Luketic S, Malcic A, Jukic S, Anic I, Segovic S, Kalenic S. Coronal microleakage of two root-end filling materials using a polymicrobial marker. J Endod 2008; 34: 201-3.
- 25. Balguerie E, van der Sluis L, Vallaeys K, Gurgel-Georgelin M, Diemer F. Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study. J Endod 2011;37:1576-9.
- 26. Sirisha K, Rambabu T, Shankar YR, Ravikumar P. Validity of bond strength tests: A critical review: Part I. J Conserv Dent 2014;17:305-11.
- 27. Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod 2013;39:669-73.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Diş Hekimliği |
| Bölüm | Araştırma |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ağustos 2019 |
| Gönderilme Tarihi | 11 Nisan 2018 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 6 Sayı: 2 |
Selcuk Dental Journal Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.